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Article: A crucial role of macrophages in the immune responses to oral DNA vaccination against hepatitis B virus in a murine model

TitleA crucial role of macrophages in the immune responses to oral DNA vaccination against hepatitis B virus in a murine model
Authors
Issue Date2001
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine
Citation
Vaccine, 2001, v. 20 n. 1-2, p. 140-147 How to Cite?
AbstractIn the previous study, we had shown that live oral vaccination with Salmonella typhimurium delivering plasmid DNA-HBsAg (oral DNA vaccine) evoked a vigorous T cell response and a weak antibody response with predominant subclass IgG2a in mice, suggesting a significant involvement by professional antigen presenting cells (APC). In the present study, this possibility was further studied by infecting peritoneal macrophages (MΦ) with the oral DNA vaccine. Although, the infected cells could only express low level of the viral antigen, they nevertheless stimulated a vigorous lymphocyte proliferation of splenocytes from immune mice, induced these cells to elaborate interferon-γ and stimulated development of HBV-specific cytotoxicity against target cells expressing the viral antigen. Infusion of the infected MΦ evoked a vigorous Th 1 and cytotoxic T lymphocyte (CTL) response and a weak IgG2a antibody response in mice, which was essentially the same as response to the oral DNA vaccine. In contrast, recombinant protein vaccine evoked a vigorous IgG1 antibody response and a weak T cell response. While, given intramuscularly, the same plasmid DNA vaccine as that contained in the oral DNA vaccine evoked a vigorous IgG1 antibody response and a moderate T cell response in these animals. It was concluded that professional APC may orchestrate the immune response to live oral DNA vaccine and it was of interest to note that different vaccine formulation and routes of administration evoke distinct immune response to HBV. © 2001 Elsevier Science Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79130
ISSN
2015 Impact Factor: 3.413
2015 SCImago Journal Rankings: 2.044
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorNg, MHen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorWong, LPen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:50:57Z-
dc.date.available2010-09-06T07:50:57Z-
dc.date.issued2001en_HK
dc.identifier.citationVaccine, 2001, v. 20 n. 1-2, p. 140-147en_HK
dc.identifier.issn0264-410Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/79130-
dc.description.abstractIn the previous study, we had shown that live oral vaccination with Salmonella typhimurium delivering plasmid DNA-HBsAg (oral DNA vaccine) evoked a vigorous T cell response and a weak antibody response with predominant subclass IgG2a in mice, suggesting a significant involvement by professional antigen presenting cells (APC). In the present study, this possibility was further studied by infecting peritoneal macrophages (MΦ) with the oral DNA vaccine. Although, the infected cells could only express low level of the viral antigen, they nevertheless stimulated a vigorous lymphocyte proliferation of splenocytes from immune mice, induced these cells to elaborate interferon-γ and stimulated development of HBV-specific cytotoxicity against target cells expressing the viral antigen. Infusion of the infected MΦ evoked a vigorous Th 1 and cytotoxic T lymphocyte (CTL) response and a weak IgG2a antibody response in mice, which was essentially the same as response to the oral DNA vaccine. In contrast, recombinant protein vaccine evoked a vigorous IgG1 antibody response and a weak T cell response. While, given intramuscularly, the same plasmid DNA vaccine as that contained in the oral DNA vaccine evoked a vigorous IgG1 antibody response and a moderate T cell response in these animals. It was concluded that professional APC may orchestrate the immune response to live oral DNA vaccine and it was of interest to note that different vaccine formulation and routes of administration evoke distinct immune response to HBV. © 2001 Elsevier Science Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccineen_HK
dc.relation.ispartofVaccineen_HK
dc.rightsVaccine. Copyright © Elsevier Ltd.en_HK
dc.subject.meshAdministration, Oralen_HK
dc.subject.meshAdoptive Transferen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCytotoxicity, Immunologicen_HK
dc.subject.meshDNA, Recombinant - administration & dosageen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHepatitis B Antibodies - biosynthesis - blood - immunologyen_HK
dc.subject.meshHepatitis B Surface Antigens - biosynthesisen_HK
dc.subject.meshHepatitis B Vaccines - administration & dosage - immunologyen_HK
dc.subject.meshImmune Toleranceen_HK
dc.subject.meshImmunization Scheduleen_HK
dc.subject.meshImmunoglobulin G - biosynthesis - blood - immunologyen_HK
dc.subject.meshInjections, Intraperitonealen_HK
dc.subject.meshInjections, Intravenousen_HK
dc.subject.meshInterferon-gamma - secretionen_HK
dc.subject.meshInterleukin-4 - secretionen_HK
dc.subject.meshLymphocyte Activationen_HK
dc.subject.meshMacrophages, Peritoneal - immunology - transplantation - virologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshPlasmids - geneticsen_HK
dc.subject.meshSalmonella typhimurium - geneticsen_HK
dc.subject.meshSpecific Pathogen-Free Organismsen_HK
dc.subject.meshT-Lymphocytes, Cytotoxic - immunologyen_HK
dc.subject.meshTh1 Cells - immunology - secretionen_HK
dc.subject.meshVaccinationen_HK
dc.subject.meshVaccines, DNA - administration & dosage - immunologyen_HK
dc.titleA crucial role of macrophages in the immune responses to oral DNA vaccination against hepatitis B virus in a murine modelen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0264-410X&volume=20&spage=140&epage=147&date=2002&atitle=A+crucial+role+of+macrophages+in+the+immune+responses+to+oral+DNA+vaccination+against+hepatitis+B+virus+in+a+murine+modelen_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0264-410X(01)00272-9en_HK
dc.identifier.pmid11567758en_HK
dc.identifier.scopuseid_2-s2.0-0035850994en_HK
dc.identifier.hkuros74412en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035850994&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume20en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage140en_HK
dc.identifier.epage147en_HK
dc.identifier.isiWOS:000171512000023-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridNg, MH=7202076421en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridWong, LP=7402092221en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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