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Article: Direct detection of Mycobacterium tuberculosis in respiratory specimens using an automated DNA amplification assay and a single tube nested polymerase chain reaction (PCR)

TitleDirect detection of Mycobacterium tuberculosis in respiratory specimens using an automated DNA amplification assay and a single tube nested polymerase chain reaction (PCR)
Authors
Issue Date1998
PublisherWalter de Gruyter GmbH & Co KG. The Journal's web site is located at http://www.degruyter.de/journals/cclm
Citation
Clinical Chemistry And Laboratory Medicine, 1998, v. 36 n. 8, p. 597-599 How to Cite?
AbstractThe performance of an automated DNA amplification assay (Roche Cobas® Amplicor(TM) Mycobacterium Tuberculosis Test (aPCR) was compared with an in-house single tube nested polymerase chain reaction (nPCR) for the direct detection of Mycobacterium tuberculosis in respiratory specimens. Among 385 specimens, 56 were culture positive for mycobacteria (44 positive for Mycobacterium tuberculosis and 12 positive for non-tuberculosis mycobacteria). The diagnostic sensitivities of aPCR and nPCR were 86% and 91% whereas a 100% diagnostic specificity of both assays was attained. By aPCR, inhibitors were detected in 6% of the clinical samples. For nPCR, the usage of a new thermostable DNA polymerase facilitated pre-PCR decontamination using uracil-N-glycosylase and 'hot start' in single step procedure. The results of the study indicated that DNA amplification assays, either manual or automated, were rapid and specific tools for diagnosing pulmonary tuberculosis.
Persistent Identifierhttp://hdl.handle.net/10722/79129
ISSN
2015 Impact Factor: 3.017
2015 SCImago Journal Rankings: 0.873
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYam, WCen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorSeto, WHen_HK
dc.date.accessioned2010-09-06T07:50:56Z-
dc.date.available2010-09-06T07:50:56Z-
dc.date.issued1998en_HK
dc.identifier.citationClinical Chemistry And Laboratory Medicine, 1998, v. 36 n. 8, p. 597-599en_HK
dc.identifier.issn1434-6621en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79129-
dc.description.abstractThe performance of an automated DNA amplification assay (Roche Cobas® Amplicor(TM) Mycobacterium Tuberculosis Test (aPCR) was compared with an in-house single tube nested polymerase chain reaction (nPCR) for the direct detection of Mycobacterium tuberculosis in respiratory specimens. Among 385 specimens, 56 were culture positive for mycobacteria (44 positive for Mycobacterium tuberculosis and 12 positive for non-tuberculosis mycobacteria). The diagnostic sensitivities of aPCR and nPCR were 86% and 91% whereas a 100% diagnostic specificity of both assays was attained. By aPCR, inhibitors were detected in 6% of the clinical samples. For nPCR, the usage of a new thermostable DNA polymerase facilitated pre-PCR decontamination using uracil-N-glycosylase and 'hot start' in single step procedure. The results of the study indicated that DNA amplification assays, either manual or automated, were rapid and specific tools for diagnosing pulmonary tuberculosis.en_HK
dc.languageengen_HK
dc.publisherWalter de Gruyter GmbH & Co KG. The Journal's web site is located at http://www.degruyter.de/journals/cclmen_HK
dc.relation.ispartofClinical Chemistry and Laboratory Medicineen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshDNA Primersen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMycobacterium tuberculosis - genetics - isolation & purificationen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshTuberculosis - diagnosis - microbiologyen_HK
dc.titleDirect detection of Mycobacterium tuberculosis in respiratory specimens using an automated DNA amplification assay and a single tube nested polymerase chain reaction (PCR)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1434-6621&volume=36&issue=8&spage=597&epage=599&date=1998&atitle=Direct+Detection+of+Mycobacterium+tuberculosis+in+Respiratory+Specimens+Using+an+Automated+DNA+Amplification+Assay+and+a+Single+Tube+Nested+Polymerase+Chain+Reaction+(PCR)en_HK
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1515/CCLM.1998.104en_HK
dc.identifier.pmid9806468-
dc.identifier.scopuseid_2-s2.0-0031718768en_HK
dc.identifier.hkuros44866en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031718768&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue8en_HK
dc.identifier.spage597en_HK
dc.identifier.epage599en_HK
dc.identifier.isiWOS:000076742100021-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridSeto, WH=7005799377en_HK

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