File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds
  • Basic View
  • Metadata View
  • XML View
TitleIdentifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds
 
AuthorsCheung, PP1
Leung, YHC1
Chow, CK1
Ng, CF1
Tsang, CL1
Wu, YO1
Ma, SK1
Sia, SF1
Guan, Y1
Peiris, JSM1 2
 
KeywordsAvian
DNA barcoding
H5N1
Human
Influenza
Surveillance
 
Issue Date2009
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
 
CitationJournal Of Clinical Virology, 2009, v. 46 n. 1, p. 90-93 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jcv.2009.06.016
 
AbstractBackground: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. Objectives: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. Study design: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. Results: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8 μg of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. Conclusion: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds. © 2009 Elsevier B.V. All rights reserved.
 
ISSN1386-6532
2013 Impact Factor: 3.466
2013 SCImago Journal Rankings: 1.812
 
DOIhttp://dx.doi.org/10.1016/j.jcv.2009.06.016
 
PubMed Central IDPMC2765912
 
ISI Accession Number IDWOS:000269426100020
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorCheung, PP
 
dc.contributor.authorLeung, YHC
 
dc.contributor.authorChow, CK
 
dc.contributor.authorNg, CF
 
dc.contributor.authorTsang, CL
 
dc.contributor.authorWu, YO
 
dc.contributor.authorMa, SK
 
dc.contributor.authorSia, SF
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorPeiris, JSM
 
dc.date.accessioned2010-09-06T07:50:51Z
 
dc.date.available2010-09-06T07:50:51Z
 
dc.date.issued2009
 
dc.description.abstractBackground: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. Objectives: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. Study design: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. Results: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8 μg of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. Conclusion: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds. © 2009 Elsevier B.V. All rights reserved.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal Of Clinical Virology, 2009, v. 46 n. 1, p. 90-93 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jcv.2009.06.016
 
dc.identifier.citeulike5165008
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jcv.2009.06.016
 
dc.identifier.epage93
 
dc.identifier.hkuros162832
 
dc.identifier.isiWOS:000269426100020
 
dc.identifier.issn1386-6532
2013 Impact Factor: 3.466
2013 SCImago Journal Rankings: 1.812
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC2765912
 
dc.identifier.pmid19604718
 
dc.identifier.scopuseid_2-s2.0-67949107937
 
dc.identifier.spage90
 
dc.identifier.urihttp://hdl.handle.net/10722/79121
 
dc.identifier.volume46
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofJournal of Clinical Virology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Clinical Virology. Copyright © Elsevier BV.
 
dc.subjectAvian
 
dc.subjectDNA barcoding
 
dc.subjectH5N1
 
dc.subjectHuman
 
dc.subjectInfluenza
 
dc.subjectSurveillance
 
dc.titleIdentifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Cheung, PP</contributor.author>
<contributor.author>Leung, YHC</contributor.author>
<contributor.author>Chow, CK</contributor.author>
<contributor.author>Ng, CF</contributor.author>
<contributor.author>Tsang, CL</contributor.author>
<contributor.author>Wu, YO</contributor.author>
<contributor.author>Ma, SK</contributor.author>
<contributor.author>Sia, SF</contributor.author>
<contributor.author>Guan, Y</contributor.author>
<contributor.author>Peiris, JSM</contributor.author>
<date.accessioned>2010-09-06T07:50:51Z</date.accessioned>
<date.available>2010-09-06T07:50:51Z</date.available>
<date.issued>2009</date.issued>
<identifier.citation>Journal Of Clinical Virology, 2009, v. 46 n. 1, p. 90-93</identifier.citation>
<identifier.issn>1386-6532</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/79121</identifier.uri>
<description.abstract>Background: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. Objectives: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. Study design: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. Results: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8 &#956;g of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. Conclusion: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds. &#169; 2009 Elsevier B.V. All rights reserved.</description.abstract>
<language>eng</language>
<publisher>Elsevier BV. The Journal&apos;s web site is located at http://www.elsevier.com/locate/jcv</publisher>
<relation.ispartof>Journal of Clinical Virology</relation.ispartof>
<rights>Journal of Clinical Virology. Copyright &#169; Elsevier BV.</rights>
<subject>Avian</subject>
<subject>DNA barcoding</subject>
<subject>H5N1</subject>
<subject>Human</subject>
<subject>Influenza</subject>
<subject>Surveillance</subject>
<title>Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds</title>
<type>Article</type>
<identifier.openurl>http://library.hku.hk:4550/resserv?sid=HKU:IR&amp;issn=1386-6532&amp;volume=46&amp;issue=1&amp;spage=90&amp;epage=93&amp;date=2009&amp;atitle=Identifying+The+Species-origin+Of+Faecal+Droppings+Used+For+Avian+Influenza+Virus+Surveillance+In+Wild-birds.</identifier.openurl>
<description.nature>link_to_OA_fulltext</description.nature>
<identifier.doi>10.1016/j.jcv.2009.06.016</identifier.doi>
<identifier.pmid>19604718</identifier.pmid>
<identifier.pmcid>PMC2765912</identifier.pmcid>
<identifier.scopus>eid_2-s2.0-67949107937</identifier.scopus>
<identifier.hkuros>162832</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-67949107937&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>46</identifier.volume>
<identifier.issue>1</identifier.issue>
<identifier.spage>90</identifier.spage>
<identifier.epage>93</identifier.epage>
<identifier.isi>WOS:000269426100020</identifier.isi>
<publisher.place>Netherlands</publisher.place>
<identifier.citeulike>5165008</identifier.citeulike>
<bitstream.url>http://hub.hku.hk/bitstream/10722/79121/1/re01.htm</bitstream.url>
</item>
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong