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Article: Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds

TitleIdentifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds
Authors
KeywordsAvian
DNA barcoding
H5N1
Human
Influenza
Surveillance
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal Of Clinical Virology, 2009, v. 46 n. 1, p. 90-93 How to Cite?
AbstractBackground: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. Objectives: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. Study design: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. Results: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8 μg of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. Conclusion: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds. © 2009 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79121
ISSN
2015 Impact Factor: 2.647
2015 SCImago Journal Rankings: 1.503
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, PPen_HK
dc.contributor.authorLeung, YHCen_HK
dc.contributor.authorChow, CKen_HK
dc.contributor.authorNg, CFen_HK
dc.contributor.authorTsang, CLen_HK
dc.contributor.authorWu, YOen_HK
dc.contributor.authorMa, SKen_HK
dc.contributor.authorSia, SFen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2010-09-06T07:50:51Z-
dc.date.available2010-09-06T07:50:51Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Clinical Virology, 2009, v. 46 n. 1, p. 90-93en_HK
dc.identifier.issn1386-6532en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79121-
dc.description.abstractBackground: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. Objectives: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. Study design: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. Results: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8 μg of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. Conclusion: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds. © 2009 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_HK
dc.relation.ispartofJournal of Clinical Virologyen_HK
dc.rightsJournal of Clinical Virology. Copyright © Elsevier BV.en_HK
dc.subjectAvianen_HK
dc.subjectDNA barcodingen_HK
dc.subjectH5N1en_HK
dc.subjectHumanen_HK
dc.subjectInfluenzaen_HK
dc.subjectSurveillanceen_HK
dc.titleIdentifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birdsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1386-6532&volume=46&issue=1&spage=90&epage=93&date=2009&atitle=Identifying+The+Species-origin+Of+Faecal+Droppings+Used+For+Avian+Influenza+Virus+Surveillance+In+Wild-birds.en_HK
dc.identifier.emailLeung, YHC: cyhleung@hkucc.hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityLeung, YHC=rp00307en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.jcv.2009.06.016en_HK
dc.identifier.pmid19604718-
dc.identifier.pmcidPMC2765912-
dc.identifier.scopuseid_2-s2.0-67949107937en_HK
dc.identifier.hkuros162832en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67949107937&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue1en_HK
dc.identifier.spage90en_HK
dc.identifier.epage93en_HK
dc.identifier.isiWOS:000269426100020-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridCheung, PP=35214597500en_HK
dc.identifier.scopusauthoridLeung, YHC=26531438300en_HK
dc.identifier.scopusauthoridChow, CK=36904165800en_HK
dc.identifier.scopusauthoridNg, CF=22135736800en_HK
dc.identifier.scopusauthoridTsang, CL=22136930900en_HK
dc.identifier.scopusauthoridWu, YO=35216685600en_HK
dc.identifier.scopusauthoridMa, SK=35215973500en_HK
dc.identifier.scopusauthoridSia, SF=8574447900en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.citeulike5165008-

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