Article: Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds

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TitleIdentifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds
AuthorsCheung, PP1
Leung, YHC1
Chow, CK1
Ng, CF1
Tsang, CL1
Wu, YO1
Ma, SK1
Sia, SF1
Guan, Y1
Peiris, JSM1 2
KeywordsAvian
DNA barcoding
H5N1
Human
Influenza
Surveillance
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
CitationJournal Of Clinical Virology, 2009, v. 46 n. 1, p. 90-93 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jcv.2009.06.016
AbstractBackground: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. Objectives: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. Study design: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. Results: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8 μg of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. Conclusion: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds. © 2009 Elsevier B.V. All rights reserved.
ISSN1386-6532
2011 Impact Factor: 3.969
2011 SCImago Journal Rankings: 0.364
DOIhttp://dx.doi.org/10.1016/j.jcv.2009.06.016
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorCheung, PP
dc.contributor.authorLeung, YHC
dc.contributor.authorChow, CK
dc.contributor.authorNg, CF
dc.contributor.authorTsang, CL
dc.contributor.authorWu, YO
dc.contributor.authorMa, SK
dc.contributor.authorSia, SF
dc.contributor.authorGuan, Y
dc.contributor.authorPeiris, JSM
dc.date.accessioned2010-09-06T07:50:51Z
dc.date.available2010-09-06T07:50:51Z
dc.date.issued2009
dc.description.abstractBackground: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. Objectives: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. Study design: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. Results: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8 μg of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. Conclusion: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds. © 2009 Elsevier B.V. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Clinical Virology, 2009, v. 46 n. 1, p. 90-93 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jcv.2009.06.016
dc.identifier.citeulike5165008
dc.identifier.doihttp://dx.doi.org/10.1016/j.jcv.2009.06.016
dc.identifier.epage93
dc.identifier.hkuros162832
dc.identifier.isiWOS:000269426100020
dc.identifier.issn1386-6532
2011 Impact Factor: 3.969
2011 SCImago Journal Rankings: 0.364
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.pmid19604718
dc.identifier.scopuseid_2-s2.0-67949107937
dc.identifier.spage90
dc.identifier.urihttp://hdl.handle.net/10722/79121
dc.identifier.volume46
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
dc.publisher.placeNetherlands
dc.relation.ispartofJournal of Clinical Virology
dc.relation.referencesReferences in Scopus
dc.rightsJournal of Clinical Virology. Copyright © Elsevier BV.
dc.subjectAvian
dc.subjectDNA barcoding
dc.subjectH5N1
dc.subjectHuman
dc.subjectInfluenza
dc.subjectSurveillance
dc.titleIdentifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong