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Article: Hemagglutinin pseudotyped lentiviral particles: Characterization of a new method for avian H5N1 influenza sero-diagnosis

TitleHemagglutinin pseudotyped lentiviral particles: Characterization of a new method for avian H5N1 influenza sero-diagnosis
Authors
KeywordsH5N1
Influenza
Pseudotyped lentiviral particles
Serodiagnostic
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal Of Clinical Virology, 2007, v. 39 n. 1, p. 27-33 How to Cite?
AbstractBackground: Highly pathogenic avian influenza (HPAI) H5N1 has spread globally in birds and infected over 270 humans with an apparently high mortality rate. Serologic studies to determine the extent of asymptomatic H5N1 infection in humans and other mammals and to investigate the immunogenicity of current H5N1 vaccine candidates have been hampered by the biosafety requirements needed for H5N1 micro-neutralization tests. Objective: Development of a serodiagnostic tool for highly pathogenic influenza that reproduces H5N1 biology but can be used with less biohazard. Study Design: We have generated and evaluated H5 hemagglutinin pseudotyped lentiviral particles encoding the luciferase reporter (H5pp). Results: H5pp entry into target cells depends on α2-3 cell surface sialic acids and requires low pH for membrane fusion. H5pp infectivity is specifically neutralized by sera from patients and animals infected with H5N1 and correlates well with conventional microneutralization test. Conclusions: H5pp reproduce H5N1 influenza virus entry into target cells and potentially provides a high-throughput and safe method for sero-epidemiology. © 2007 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79096
ISSN
2015 Impact Factor: 2.647
2015 SCImago Journal Rankings: 1.503
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNefkens, Ien_HK
dc.contributor.authorGarcia, JMen_HK
dc.contributor.authorLing, CSen_HK
dc.contributor.authorLagarde, Nen_HK
dc.contributor.authorNicholls, Jen_HK
dc.contributor.authorTang, DJen_HK
dc.contributor.authorPeiris, Men_HK
dc.contributor.authorBuchy, Pen_HK
dc.contributor.authorAltmeyer, Ren_HK
dc.date.accessioned2010-09-06T07:50:32Z-
dc.date.available2010-09-06T07:50:32Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Clinical Virology, 2007, v. 39 n. 1, p. 27-33en_HK
dc.identifier.issn1386-6532en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79096-
dc.description.abstractBackground: Highly pathogenic avian influenza (HPAI) H5N1 has spread globally in birds and infected over 270 humans with an apparently high mortality rate. Serologic studies to determine the extent of asymptomatic H5N1 infection in humans and other mammals and to investigate the immunogenicity of current H5N1 vaccine candidates have been hampered by the biosafety requirements needed for H5N1 micro-neutralization tests. Objective: Development of a serodiagnostic tool for highly pathogenic influenza that reproduces H5N1 biology but can be used with less biohazard. Study Design: We have generated and evaluated H5 hemagglutinin pseudotyped lentiviral particles encoding the luciferase reporter (H5pp). Results: H5pp entry into target cells depends on α2-3 cell surface sialic acids and requires low pH for membrane fusion. H5pp infectivity is specifically neutralized by sera from patients and animals infected with H5N1 and correlates well with conventional microneutralization test. Conclusions: H5pp reproduce H5N1 influenza virus entry into target cells and potentially provides a high-throughput and safe method for sero-epidemiology. © 2007 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_HK
dc.relation.ispartofJournal of Clinical Virologyen_HK
dc.rightsJournal of Clinical Virology. Copyright © Elsevier BV.en_HK
dc.subjectH5N1en_HK
dc.subjectInfluenzaen_HK
dc.subjectPseudotyped lentiviral particlesen_HK
dc.subjectSerodiagnosticen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBirdsen_HK
dc.subject.meshDogsen_HK
dc.subject.meshHemagglutinin Glycoproteins, Influenza Virus - biosynthesis - genetics - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInfluenza A Virus, H5N1 Subtype - genetics - pathogenicity - physiologyen_HK
dc.subject.meshInfluenza in Birds - blood - diagnosis - virologyen_HK
dc.subject.meshInfluenza, Human - blood - diagnosis - virologyen_HK
dc.subject.meshLentivirus - genetics - pathogenicity - physiologyen_HK
dc.subject.meshLuciferases - geneticsen_HK
dc.subject.meshNeutralization Tests - methodsen_HK
dc.subject.meshSerologic Tests - methodsen_HK
dc.subject.meshVirion - genetics - pathogenicity - physiologyen_HK
dc.titleHemagglutinin pseudotyped lentiviral particles: Characterization of a new method for avian H5N1 influenza sero-diagnosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1386-6532&volume=39&issue=1&spage=27&epage=33&date=2007&atitle=Hemagglutinin+pseudotyped+lentiviral+particles:+Characterization+of+a+new+method+for+avian+H5N1+influenza+sero-diagnosisen_HK
dc.identifier.emailNicholls, J: jmnichol@hkucc.hku.hken_HK
dc.identifier.emailPeiris, M: malik@hkucc.hku.hken_HK
dc.identifier.authorityNicholls, J=rp00364en_HK
dc.identifier.authorityPeiris, M=rp00410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jcv.2007.02.005en_HK
dc.identifier.pmid17409017-
dc.identifier.scopuseid_2-s2.0-34147150185en_HK
dc.identifier.hkuros147215en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34147150185&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue1en_HK
dc.identifier.spage27en_HK
dc.identifier.epage33en_HK
dc.identifier.isiWOS:000248631300005-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridNefkens, I=6508225525en_HK
dc.identifier.scopusauthoridGarcia, JM=16202601400en_HK
dc.identifier.scopusauthoridLing, CS=16203123400en_HK
dc.identifier.scopusauthoridLagarde, N=16203012000en_HK
dc.identifier.scopusauthoridNicholls, J=7201463077en_HK
dc.identifier.scopusauthoridTang, DJ=55167386100en_HK
dc.identifier.scopusauthoridPeiris, M=7005486823en_HK
dc.identifier.scopusauthoridBuchy, P=15019240000en_HK
dc.identifier.scopusauthoridAltmeyer, R=7003677186en_HK

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