File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Differential fadE28 expression associated with phenotypic virulence of Mycobacterium tuberculosis

TitleDifferential fadE28 expression associated with phenotypic virulence of Mycobacterium tuberculosis
Authors
KeywordsDifferential gene expression
fadE28
Hypervirulent strain
Mycobacterium tuberculosis
Issue Date2008
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/micpath
Citation
Microbial Pathogenesis, 2008, v. 45 n. 1, p. 12-17 How to Cite?
AbstractAbility to persist in human macrophages is central to the virulence of Mycobacterium tuberculosis and is not invariable among various strains. Differential gene expression that is associated with phenotypic virulence may provide additional information of virulent genes involved in the pathogenesis of M. tuberculosis, which is not fully elucidated. Three hypervirulent strains of M. tuberculosis isolated from patients suffering with tuberculous meningitis were shown to grow more rapidly inside human macrophages in a previous study. In the current investigation, expression of 7 mycobacterial genes (fadE28, mce1A, mymA, acr, sigA, sugC, and Rv3723) of these strains during ex vivo macrophage challenge and in vitro acid shock was quantified by real-time PCR. Using rrs gene as a normalisation gene, fadE28 gene exhibited differential gene expression that is associated with phenotypic virulence, whereas the other 6 genes showed indistinguishable expression patterns. Up-regulation of fadE28 gene in the hypervirulent strains may account for virulence by increasing the efficiency of beta-oxidation, which is important for the persistence in macrophages as M. tuberculosis uses fatty acids preferably inside phagosome of macrophages. The fadE28 gene, together with its adjacent genes may also be critical in the process of lipid modification that could facilitate parasitism in human macrophages. © 2008 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79092
ISSN
2015 Impact Factor: 1.888
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLam, THJen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorWong, KCen_HK
dc.contributor.authorLeong, WMen_HK
dc.contributor.authorLaw, HKWen_HK
dc.contributor.authorWeng, XHen_HK
dc.contributor.authorZhang, WHen_HK
dc.contributor.authorChen, Sen_HK
dc.contributor.authorYam, WCen_HK
dc.date.accessioned2010-09-06T07:50:30Z-
dc.date.available2010-09-06T07:50:30Z-
dc.date.issued2008en_HK
dc.identifier.citationMicrobial Pathogenesis, 2008, v. 45 n. 1, p. 12-17en_HK
dc.identifier.issn0882-4010en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79092-
dc.description.abstractAbility to persist in human macrophages is central to the virulence of Mycobacterium tuberculosis and is not invariable among various strains. Differential gene expression that is associated with phenotypic virulence may provide additional information of virulent genes involved in the pathogenesis of M. tuberculosis, which is not fully elucidated. Three hypervirulent strains of M. tuberculosis isolated from patients suffering with tuberculous meningitis were shown to grow more rapidly inside human macrophages in a previous study. In the current investigation, expression of 7 mycobacterial genes (fadE28, mce1A, mymA, acr, sigA, sugC, and Rv3723) of these strains during ex vivo macrophage challenge and in vitro acid shock was quantified by real-time PCR. Using rrs gene as a normalisation gene, fadE28 gene exhibited differential gene expression that is associated with phenotypic virulence, whereas the other 6 genes showed indistinguishable expression patterns. Up-regulation of fadE28 gene in the hypervirulent strains may account for virulence by increasing the efficiency of beta-oxidation, which is important for the persistence in macrophages as M. tuberculosis uses fatty acids preferably inside phagosome of macrophages. The fadE28 gene, together with its adjacent genes may also be critical in the process of lipid modification that could facilitate parasitism in human macrophages. © 2008 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/micpathen_HK
dc.relation.ispartofMicrobial Pathogenesisen_HK
dc.subjectDifferential gene expressionen_HK
dc.subjectfadE28en_HK
dc.subjectHypervirulent strainen_HK
dc.subjectMycobacterium tuberculosisen_HK
dc.subject.meshAcyl-CoA Dehydrogenase - genetics - metabolism-
dc.subject.meshGene Expression Regulation, Bacterial-
dc.subject.meshMycobacterium tuberculosis - classification - genetics - metabolism - pathogenicity-
dc.subject.meshTuberculosis, Meningeal - microbiology-
dc.subject.meshUp-Regulation-
dc.titleDifferential fadE28 expression associated with phenotypic virulence of Mycobacterium tuberculosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0882-4010&volume=45&issue=1&spage=12&epage=17&date=2008&atitle=Differential+fadE28+expression+associated+with+phenotypic+virulence+of+Mycobacterium+tuberculosisen_HK
dc.identifier.emailLam, THJ:hrmrlth@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_HK
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_HK
dc.identifier.authorityLam, THJ=rp00326en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.micpath.2008.01.006en_HK
dc.identifier.pmid18486437-
dc.identifier.scopuseid_2-s2.0-44649197096en_HK
dc.identifier.hkuros142743en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-44649197096&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue1en_HK
dc.identifier.spage12en_HK
dc.identifier.epage17en_HK
dc.identifier.isiWOS:000262591400003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLam, THJ=7202522876en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridWong, KC=7404759813en_HK
dc.identifier.scopusauthoridLeong, WM=22941186900en_HK
dc.identifier.scopusauthoridLaw, HKW=7101939394en_HK
dc.identifier.scopusauthoridWeng, XH=7102593970en_HK
dc.identifier.scopusauthoridZhang, WH=15046133300en_HK
dc.identifier.scopusauthoridChen, S=15831031400en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats