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Article: Newly discovered coronavirus as the primary cause of severe acute respiratory syndrome

TitleNewly discovered coronavirus as the primary cause of severe acute respiratory syndrome
Authors
Issue Date2003
PublisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lancet
Citation
Lancet, 2003, v. 362 n. 9380, p. 263-270 How to Cite?
AbstractBackground: The worldwide outbreak of severe acute respiratory syndrome (SARS) is associated with a newly discovered coronavirus, SARS-associated coronavirus (SARS-CoV). We did clinical and experimental studies to assess the role of this virus in the cause of SARS. Methods: We tested clinical and postmortem samples from 436 SARS patients in six countries for infection with SARS-CoV, human metapneumovirus, and other respiratory pathogens. We infected four cynomolgus macaques (Macaca fascicularis) with SARS-CoV in an attempt to replicate SARS and did necropsies on day 6 after infection. Findings: SARS-CoV infection was diagnosed in 329 (75%) of 436 patients fitting the case definition of SARS; human metapneumovirus was diagnosed in 41 (12%) of 335, and other respiratory pathogens were diagnosed only sporadically. SARS-CoV was, therefore, the most likely causal agent of SARS. The four SARS-CoV-infected macaques excreted SARS-CoV from nose, mouth, and pharynx from 2 days after infection. Three of four macaques developed diffuse alveolar damage, similar to that in SARS patients, and characterised by epithelial necrosis, serosanguineous exudate, formation of hyaline membranes, type 2 pneumocyte hyperplasia, and the presence of syncytia. SARS-CoV was detected in pneumonic areas by virus isolation and RT-PCR, and was localised to alveolar epithelial cells and syncytia by immunohistochemistry and transmission electron microscopy. Interpretation: Replication in SARS-CoV-infected macaques of pneumonia similar to that in human beings with SARS, combined with the high prevalence of SARS-CoV infection in SARS patients, fulfill the criteria required to prove that SARS-CoV is the primary cause of SARS.
Persistent Identifierhttp://hdl.handle.net/10722/79062
ISSN
2015 Impact Factor: 44.002
2015 SCImago Journal Rankings: 14.638
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKuiken, Ten_HK
dc.contributor.authorFouchier, RAMen_HK
dc.contributor.authorSchutten, Men_HK
dc.contributor.authorRimmelzwaan, GFen_HK
dc.contributor.authorVan Amerongen, Gen_HK
dc.contributor.authorVan Riel, Den_HK
dc.contributor.authorLaman, JDen_HK
dc.contributor.authorDe Jong, Ten_HK
dc.contributor.authorVan Doornum, Gen_HK
dc.contributor.authorLim, Wen_HK
dc.contributor.authorLing, AEen_HK
dc.contributor.authorChan, PKSen_HK
dc.contributor.authorTam, JSen_HK
dc.contributor.authorZambon, MCen_HK
dc.contributor.authorGopal, Ren_HK
dc.contributor.authorDrosten, Cen_HK
dc.contributor.authorVan Der Werf, Sen_HK
dc.contributor.authorEscriou, Nen_HK
dc.contributor.authorManuguerra, JCen_HK
dc.contributor.authorStöhr, Ken_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorOsterhaus, ADMEen_HK
dc.date.accessioned2010-09-06T07:50:08Z-
dc.date.available2010-09-06T07:50:08Z-
dc.date.issued2003en_HK
dc.identifier.citationLancet, 2003, v. 362 n. 9380, p. 263-270en_HK
dc.identifier.issn0140-6736en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79062-
dc.description.abstractBackground: The worldwide outbreak of severe acute respiratory syndrome (SARS) is associated with a newly discovered coronavirus, SARS-associated coronavirus (SARS-CoV). We did clinical and experimental studies to assess the role of this virus in the cause of SARS. Methods: We tested clinical and postmortem samples from 436 SARS patients in six countries for infection with SARS-CoV, human metapneumovirus, and other respiratory pathogens. We infected four cynomolgus macaques (Macaca fascicularis) with SARS-CoV in an attempt to replicate SARS and did necropsies on day 6 after infection. Findings: SARS-CoV infection was diagnosed in 329 (75%) of 436 patients fitting the case definition of SARS; human metapneumovirus was diagnosed in 41 (12%) of 335, and other respiratory pathogens were diagnosed only sporadically. SARS-CoV was, therefore, the most likely causal agent of SARS. The four SARS-CoV-infected macaques excreted SARS-CoV from nose, mouth, and pharynx from 2 days after infection. Three of four macaques developed diffuse alveolar damage, similar to that in SARS patients, and characterised by epithelial necrosis, serosanguineous exudate, formation of hyaline membranes, type 2 pneumocyte hyperplasia, and the presence of syncytia. SARS-CoV was detected in pneumonic areas by virus isolation and RT-PCR, and was localised to alveolar epithelial cells and syncytia by immunohistochemistry and transmission electron microscopy. Interpretation: Replication in SARS-CoV-infected macaques of pneumonia similar to that in human beings with SARS, combined with the high prevalence of SARS-CoV infection in SARS patients, fulfill the criteria required to prove that SARS-CoV is the primary cause of SARS.en_HK
dc.languageengen_HK
dc.publisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lanceten_HK
dc.relation.ispartofLanceten_HK
dc.titleNewly discovered coronavirus as the primary cause of severe acute respiratory syndromeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0140-6736&volume=362 &issue=9380&spage=263&epage=70&date=2003&atitle=Newly+discovered+coronavirus+as+the+primary+cause+of+severe+acute+respiratory+syndrome.en_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0140-6736(03)13967-0en_HK
dc.identifier.pmid12892955-
dc.identifier.scopuseid_2-s2.0-0042198682en_HK
dc.identifier.hkuros83116en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0042198682&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume362en_HK
dc.identifier.issue9380en_HK
dc.identifier.spage263en_HK
dc.identifier.epage270en_HK
dc.identifier.isiWOS:000184413400007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridKuiken, T=26643529400en_HK
dc.identifier.scopusauthoridFouchier, RAM=7006060466en_HK
dc.identifier.scopusauthoridSchutten, M=7004005251en_HK
dc.identifier.scopusauthoridRimmelzwaan, GF=7005416180en_HK
dc.identifier.scopusauthoridVan Amerongen, G=7004695265en_HK
dc.identifier.scopusauthoridVan Riel, D=6602932722en_HK
dc.identifier.scopusauthoridLaman, JD=21635097100en_HK
dc.identifier.scopusauthoridDe Jong, T=7201837394en_HK
dc.identifier.scopusauthoridVan Doornum, G=7005445921en_HK
dc.identifier.scopusauthoridLim, W=7202378277en_HK
dc.identifier.scopusauthoridLing, AE=7102194546en_HK
dc.identifier.scopusauthoridChan, PKS=7403497792en_HK
dc.identifier.scopusauthoridTam, JS=24788939600en_HK
dc.identifier.scopusauthoridZambon, MC=7006818684en_HK
dc.identifier.scopusauthoridGopal, R=7102284995en_HK
dc.identifier.scopusauthoridDrosten, C=7003813990en_HK
dc.identifier.scopusauthoridVan Der Werf, S=7005851162en_HK
dc.identifier.scopusauthoridEscriou, N=6603606703en_HK
dc.identifier.scopusauthoridManuguerra, JC=7003610543en_HK
dc.identifier.scopusauthoridStöhr, K=7004827453en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridOsterhaus, ADME=7202908501en_HK

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