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Article: Serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: Implications for early diagnosis and serotyping of dengue virus infections

TitleSerotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: Implications for early diagnosis and serotyping of dengue virus infections
Authors
Issue Date2006
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2006, v. 44 n. 8, p. 2872-2878 How to Cite?
AbstractRapid diagnosis and serotyping of dengue virus (DV) infections are important for timely clinical management and epidemiological control in areas where multiple flaviviruses are endemic. However, the speed and accuracy of diagnosis must be balanced against test cost and availability, especially in developing countries. We developed a specific antigen capture enzyme-linked immunosorbent assay (ELISA) for early detection and serotyping of DV serotype 1 (DV1) by using well-characterized monoclonal antibodies (MAbs) specific to nonstructural protein 1 (NS1) of DV1. With this assay, a total of 462 serum specimens from clinically probable DV1-infected patients during the DV1 epidemic in Guangdong, China, in 2002 and 2003 were analyzed. DV1 NS1 was detectable in blood circulation from the first day up to day 18 after onset of symptoms, with a peak at days 6 to 10. The sensitivity of DV1 NS1 detection in serum specimens with reference to results from reverse transcriptase PCR was 82%, and the specificity was 98.9% with reference to 469 healthy blood donors. No cross-reactions with any of the other three DV serotypes or other closely related members of the genus Flavivirus (Japanese encephalitis virus and Yellow fever virus) were observed when tested with the clinical specimens or virus cultures. These findings suggest that the serotype-specific MAb-based NS1 antigen capture ELISA may be a valuable tool for early diagnosis and serotyping of DV infections, while also providing a standardized assay for the analysis of a great number of clinical samples with convenience and cost-effectiveness. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79051
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, Hen_HK
dc.contributor.authorDi, Ben_HK
dc.contributor.authorPan, YXen_HK
dc.contributor.authorQiu, LWen_HK
dc.contributor.authorWang, YDen_HK
dc.contributor.authorHao, Wen_HK
dc.contributor.authorHe, LJen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorChe, XYen_HK
dc.date.accessioned2010-09-06T07:50:00Z-
dc.date.available2010-09-06T07:50:00Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2006, v. 44 n. 8, p. 2872-2878en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79051-
dc.description.abstractRapid diagnosis and serotyping of dengue virus (DV) infections are important for timely clinical management and epidemiological control in areas where multiple flaviviruses are endemic. However, the speed and accuracy of diagnosis must be balanced against test cost and availability, especially in developing countries. We developed a specific antigen capture enzyme-linked immunosorbent assay (ELISA) for early detection and serotyping of DV serotype 1 (DV1) by using well-characterized monoclonal antibodies (MAbs) specific to nonstructural protein 1 (NS1) of DV1. With this assay, a total of 462 serum specimens from clinically probable DV1-infected patients during the DV1 epidemic in Guangdong, China, in 2002 and 2003 were analyzed. DV1 NS1 was detectable in blood circulation from the first day up to day 18 after onset of symptoms, with a peak at days 6 to 10. The sensitivity of DV1 NS1 detection in serum specimens with reference to results from reverse transcriptase PCR was 82%, and the specificity was 98.9% with reference to 469 healthy blood donors. No cross-reactions with any of the other three DV serotypes or other closely related members of the genus Flavivirus (Japanese encephalitis virus and Yellow fever virus) were observed when tested with the clinical specimens or virus cultures. These findings suggest that the serotype-specific MAb-based NS1 antigen capture ELISA may be a valuable tool for early diagnosis and serotyping of DV infections, while also providing a standardized assay for the analysis of a great number of clinical samples with convenience and cost-effectiveness. Copyright © 2006, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of clinical microbiology. Copyright © American Society for Microbiology.en_HK
dc.subject.meshAntibodies, Monoclonalen_HK
dc.subject.meshAntibodies, Viralen_HK
dc.subject.meshAntigens, Viral - blooden_HK
dc.subject.meshChinaen_HK
dc.subject.meshCross Reactionsen_HK
dc.subject.meshDengue - diagnosis - virologyen_HK
dc.subject.meshDengue Virus - isolation & purificationen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assay - methodsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshSerum - virologyen_HK
dc.subject.meshViral Nonstructural Proteins - blooden_HK
dc.titleSerotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: Implications for early diagnosis and serotyping of dengue virus infectionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=44&issue=8&spage=2872&epage=2878&date=2006&atitle=Serotype+1-specific+monoclonal+antibody-based+antigen+capture+immunoassay+for+detection+of+circulating+nonstructural+protein+NS1:+Implications+for+early+diagnosis+and+serotyping+of+dengue+virus+infections.en_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/JCM.00777-06en_HK
dc.identifier.pmid16891505en_HK
dc.identifier.scopuseid_2-s2.0-33747184862en_HK
dc.identifier.hkuros120586en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33747184862&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume44en_HK
dc.identifier.issue8en_HK
dc.identifier.spage2872en_HK
dc.identifier.epage2878en_HK
dc.identifier.isiWOS:000239982800029-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXu, H=8401769300en_HK
dc.identifier.scopusauthoridDi, B=6602190900en_HK
dc.identifier.scopusauthoridPan, YX=35765899800en_HK
dc.identifier.scopusauthoridQiu, LW=8719102700en_HK
dc.identifier.scopusauthoridWang, YD=9638471800en_HK
dc.identifier.scopusauthoridHao, W=7101686587en_HK
dc.identifier.scopusauthoridHe, LJ=27168813800en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridChe, XY=7005743182en_HK

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