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Article: Rapid Mycobacterium species assignment and unambiguous identification of mutations associated with antimicrobial resistance in Mycobacterium tuberculosis by automated DNA sequencing

TitleRapid Mycobacterium species assignment and unambiguous identification of mutations associated with antimicrobial resistance in Mycobacterium tuberculosis by automated DNA sequencing
Authors
Issue Date1995
PublisherCollege of American Pathologists. The Journal's web site is located at http://arpa.allenpress.com/arpaonline/?request=index-html
Citation
Archives Of Pathology And Laboratory Medicine, 1995, v. 119 n. 2, p. 131-138 How to Cite?
AbstractObjective.-To develop and demonstrate the utility of automated DNA sequencing strategies for rapid and un-ambiguous identification of Mycobacterium species and mutations associated with antimicrobial resistance in Mycobacterium tuberculosis. Design and Specimens.-A 360-base pair segment of the gene (hsp65) encoding a 65-kd heat shock protein was characterized from 91 isolates assigned to 24 Mycobacterium species by traditional biochemical techniques. Areas of seven genes recently shown to contain mutations associated with antimicrobial resistance in M tuberculosis strains were also sequenced in a sample of 128 resistant organisms. Early positive BACTEC 460 cultures and acid-fast, bacterium-positive sputum specimens from patients with tuberculosis were also studied. Results.-Automated DNA sequencing identified species-specific polymorphism in the target segment of hsp65, successfully identified organisms to the species level in smear- positive sputum samples, and unambiguously characterized seven genes associated with antimicrobial resistance in M tuberculosis. Conclusions.- Rapid identification of M tuberculosis and other Mycobacterium species is possible by automated DNA sequencing of a portion of hsp65. The technique is also feasible for analysis of some smear-positive sputum specimens. Unambiguous characterization of target segments of genes harboring mutations associated with anti-microbial resistance in M tuberculosis is possible from primary patient specimens. Taken together, the data demonstrate the feasibility of mycobacterial species identification and potential to identify mutations associated with antimicrobial resistance in less than 48 hours.
Persistent Identifierhttp://hdl.handle.net/10722/79023
ISSN
2015 Impact Factor: 2.631
2015 SCImago Journal Rankings: 1.158
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKapur, Ven_HK
dc.contributor.authorLi, LLen_HK
dc.contributor.authorHamrick, MRen_HK
dc.contributor.authorPlikaytis, BBen_HK
dc.contributor.authorShinnick, TMen_HK
dc.contributor.authorTelenti, Aen_HK
dc.contributor.authorJacobs Jr, WRen_HK
dc.contributor.authorBanerjee, Aen_HK
dc.contributor.authorCole, Sen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorClarridge III, JEen_HK
dc.contributor.authorKreiswirth, BNen_HK
dc.contributor.authorMusser, JMen_HK
dc.date.accessioned2010-09-06T07:49:39Z-
dc.date.available2010-09-06T07:49:39Z-
dc.date.issued1995en_HK
dc.identifier.citationArchives Of Pathology And Laboratory Medicine, 1995, v. 119 n. 2, p. 131-138en_HK
dc.identifier.issn0003-9985en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79023-
dc.description.abstractObjective.-To develop and demonstrate the utility of automated DNA sequencing strategies for rapid and un-ambiguous identification of Mycobacterium species and mutations associated with antimicrobial resistance in Mycobacterium tuberculosis. Design and Specimens.-A 360-base pair segment of the gene (hsp65) encoding a 65-kd heat shock protein was characterized from 91 isolates assigned to 24 Mycobacterium species by traditional biochemical techniques. Areas of seven genes recently shown to contain mutations associated with antimicrobial resistance in M tuberculosis strains were also sequenced in a sample of 128 resistant organisms. Early positive BACTEC 460 cultures and acid-fast, bacterium-positive sputum specimens from patients with tuberculosis were also studied. Results.-Automated DNA sequencing identified species-specific polymorphism in the target segment of hsp65, successfully identified organisms to the species level in smear- positive sputum samples, and unambiguously characterized seven genes associated with antimicrobial resistance in M tuberculosis. Conclusions.- Rapid identification of M tuberculosis and other Mycobacterium species is possible by automated DNA sequencing of a portion of hsp65. The technique is also feasible for analysis of some smear-positive sputum specimens. Unambiguous characterization of target segments of genes harboring mutations associated with anti-microbial resistance in M tuberculosis is possible from primary patient specimens. Taken together, the data demonstrate the feasibility of mycobacterial species identification and potential to identify mutations associated with antimicrobial resistance in less than 48 hours.en_HK
dc.languageengen_HK
dc.publisherCollege of American Pathologists. The Journal's web site is located at http://arpa.allenpress.com/arpaonline/?request=index-htmlen_HK
dc.relation.ispartofArchives of Pathology and Laboratory Medicineen_HK
dc.subject.meshAllelesen_HK
dc.subject.meshBacteriological Techniquesen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshDrug Resistance, Microbial - geneticsen_HK
dc.subject.meshGenes, Bacterial - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshMutation - geneticsen_HK
dc.subject.meshMycobacterium - genetics - isolation & purificationen_HK
dc.subject.meshMycobacterium tuberculosis - drug effects - geneticsen_HK
dc.subject.meshSequence Analysis, DNA - methodsen_HK
dc.subject.meshSputum - microbiologyen_HK
dc.titleRapid Mycobacterium species assignment and unambiguous identification of mutations associated with antimicrobial resistance in Mycobacterium tuberculosis by automated DNA sequencingen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0363-0153&volume=119&issue=2&spage=131&epage=138&date=1995&atitle=Rapid+mycobacterium+species+assignment+and+unambiguous+identification+of+mutations+associated+with+antimicrobial+resistance+in+Mycobacterium+tuberculosis+by+automated+DNA+sequencingen_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid7848059en_HK
dc.identifier.scopuseid_2-s2.0-0028817097en_HK
dc.identifier.hkuros4362en_HK
dc.identifier.volume119en_HK
dc.identifier.issue2en_HK
dc.identifier.spage131en_HK
dc.identifier.epage138en_HK
dc.identifier.isiWOS:A1995QF54000007-
dc.identifier.scopusauthoridKapur, V=7103095258en_HK
dc.identifier.scopusauthoridLi, LL=36079134300en_HK
dc.identifier.scopusauthoridHamrick, MR=7006335400en_HK
dc.identifier.scopusauthoridPlikaytis, BB=7006302571en_HK
dc.identifier.scopusauthoridShinnick, TM=7005115736en_HK
dc.identifier.scopusauthoridTelenti, A=7006764406en_HK
dc.identifier.scopusauthoridJacobs Jr, WR=10240378100en_HK
dc.identifier.scopusauthoridBanerjee, A=7402886340en_HK
dc.identifier.scopusauthoridCole, S=7401503569en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridClarridge III, JE=7005521644en_HK
dc.identifier.scopusauthoridKreiswirth, BN=7004940390en_HK
dc.identifier.scopusauthoridMusser, JM=7103224550en_HK

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