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Article: Early diagnosis of Exophiala CAPD peritonitis by 18S ribosomal RNA gene sequencing and its clinical significance
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TitleEarly diagnosis of Exophiala CAPD peritonitis by 18S ribosomal RNA gene sequencing and its clinical significance
 
AuthorsLau, SKP1
Woo, PCY1
Chiu, SK2
Leung, KW1
Yung, RWH2
Yuen, KY1 3
 
Issue Date2003
 
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobio
 
CitationDiagnostic Microbiology And Infectious Disease, 2003, v. 46 n. 2, p. 95-102 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0732-8893(03)00014-2
 
AbstractPhenotypic identification of fungi in clinical microbiology laboratoreis is often difficult and late, especially for slow growing and rarely encountered fungi. We describe the application of 18S ribosomal RNA (rRNA) gene sequencing in the early diagnosis of a case of Exophiala peritonitis. A yeast-like fungus was isolated from the dialysate fluid of a 66-year-old man undergoing continuous ambulatory peritoneal dialysis. It grew slowly after 12 days of incubation to yield mature cultures to permit recognition of microscopic features resembling those of Exophiala, a dematiacerous mold. 18S rRNA gene sequencing provided results 12 days earlier than phenotypic identification and revealed 15 base difference (0.9%) between the isolate and Exophiala sp. strain GHP 1205 (GenBank Accession no. AJ232954), indicating that the isolate most closely resembles a strain of Exophiala species. The patient responded to 4 weeks of intravenous amphotericin B therapy. Early identification of the fungus was important for the choice of anti-fungal regimen. As opportunistic fungal infections in immunocompromised patients are globally emerging problems, the development of molecular techniques for fungal identification is crucial for early diagnosis and appropriate treatment. © 2003 Elsevier Science Inc. All rights reserved.
 
ISSN0732-8893
2013 Impact Factor: 2.568
2013 SCImago Journal Rankings: 1.275
 
DOIhttp://dx.doi.org/10.1016/S0732-8893(03)00014-2
 
ISI Accession Number IDWOS:000183651500003
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLau, SKP
 
dc.contributor.authorWoo, PCY
 
dc.contributor.authorChiu, SK
 
dc.contributor.authorLeung, KW
 
dc.contributor.authorYung, RWH
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2010-09-06T07:49:32Z
 
dc.date.available2010-09-06T07:49:32Z
 
dc.date.issued2003
 
dc.description.abstractPhenotypic identification of fungi in clinical microbiology laboratoreis is often difficult and late, especially for slow growing and rarely encountered fungi. We describe the application of 18S ribosomal RNA (rRNA) gene sequencing in the early diagnosis of a case of Exophiala peritonitis. A yeast-like fungus was isolated from the dialysate fluid of a 66-year-old man undergoing continuous ambulatory peritoneal dialysis. It grew slowly after 12 days of incubation to yield mature cultures to permit recognition of microscopic features resembling those of Exophiala, a dematiacerous mold. 18S rRNA gene sequencing provided results 12 days earlier than phenotypic identification and revealed 15 base difference (0.9%) between the isolate and Exophiala sp. strain GHP 1205 (GenBank Accession no. AJ232954), indicating that the isolate most closely resembles a strain of Exophiala species. The patient responded to 4 weeks of intravenous amphotericin B therapy. Early identification of the fungus was important for the choice of anti-fungal regimen. As opportunistic fungal infections in immunocompromised patients are globally emerging problems, the development of molecular techniques for fungal identification is crucial for early diagnosis and appropriate treatment. © 2003 Elsevier Science Inc. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationDiagnostic Microbiology And Infectious Disease, 2003, v. 46 n. 2, p. 95-102 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0732-8893(03)00014-2
 
dc.identifier.doihttp://dx.doi.org/10.1016/S0732-8893(03)00014-2
 
dc.identifier.epage102
 
dc.identifier.hkuros80124
 
dc.identifier.isiWOS:000183651500003
 
dc.identifier.issn0732-8893
2013 Impact Factor: 2.568
2013 SCImago Journal Rankings: 1.275
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmid12812724
 
dc.identifier.scopuseid_2-s2.0-0038172618
 
dc.identifier.spage95
 
dc.identifier.urihttp://hdl.handle.net/10722/79013
 
dc.identifier.volume46
 
dc.languageeng
 
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobio
 
dc.publisher.placeUnited States
 
dc.relation.ispartofDiagnostic Microbiology and Infectious Disease
 
dc.relation.referencesReferences in Scopus
 
dc.rightsDiagnostic Microbiology and Infectious Disease. Copyright © Elsevier Inc.
 
dc.subject.meshAged
 
dc.subject.meshAmphotericin B - administration & dosage
 
dc.subject.meshBase Sequence
 
dc.subject.meshDialysis Solutions
 
dc.subject.meshExophiala - drug effects - isolation & purification
 
dc.subject.meshGenes, rRNA - genetics
 
dc.subject.meshHumans
 
dc.subject.meshKidney Failure, Chronic - diagnosis - therapy
 
dc.subject.meshMale
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshMycoses - diagnosis - drug therapy
 
dc.subject.meshPeritoneal Dialysis, Continuous Ambulatory - adverse effects
 
dc.subject.meshPeritonitis - diagnosis - drug therapy - etiology
 
dc.subject.meshPolymerase Chain Reaction - methods
 
dc.subject.meshRNA, Ribosomal, 18S - analysis - genetics
 
dc.subject.meshRisk Assessment
 
dc.subject.meshSensitivity and Specificity
 
dc.subject.meshTreatment Outcome
 
dc.titleEarly diagnosis of Exophiala CAPD peritonitis by 18S ribosomal RNA gene sequencing and its clinical significance
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Pamela Youde Nethersole Eastern Hospital
  3. HKU-Pasteur Research Centre