File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Early diagnosis of Exophiala CAPD peritonitis by 18S ribosomal RNA gene sequencing and its clinical significance

TitleEarly diagnosis of Exophiala CAPD peritonitis by 18S ribosomal RNA gene sequencing and its clinical significance
Authors
Issue Date2003
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobio
Citation
Diagnostic Microbiology And Infectious Disease, 2003, v. 46 n. 2, p. 95-102 How to Cite?
AbstractPhenotypic identification of fungi in clinical microbiology laboratoreis is often difficult and late, especially for slow growing and rarely encountered fungi. We describe the application of 18S ribosomal RNA (rRNA) gene sequencing in the early diagnosis of a case of Exophiala peritonitis. A yeast-like fungus was isolated from the dialysate fluid of a 66-year-old man undergoing continuous ambulatory peritoneal dialysis. It grew slowly after 12 days of incubation to yield mature cultures to permit recognition of microscopic features resembling those of Exophiala, a dematiacerous mold. 18S rRNA gene sequencing provided results 12 days earlier than phenotypic identification and revealed 15 base difference (0.9%) between the isolate and Exophiala sp. strain GHP 1205 (GenBank Accession no. AJ232954), indicating that the isolate most closely resembles a strain of Exophiala species. The patient responded to 4 weeks of intravenous amphotericin B therapy. Early identification of the fungus was important for the choice of anti-fungal regimen. As opportunistic fungal infections in immunocompromised patients are globally emerging problems, the development of molecular techniques for fungal identification is crucial for early diagnosis and appropriate treatment. © 2003 Elsevier Science Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79013
ISSN
2015 Impact Factor: 2.45
2015 SCImago Journal Rankings: 1.142
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorChiu, SKen_HK
dc.contributor.authorLeung, KWen_HK
dc.contributor.authorYung, RWHen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:49:32Z-
dc.date.available2010-09-06T07:49:32Z-
dc.date.issued2003en_HK
dc.identifier.citationDiagnostic Microbiology And Infectious Disease, 2003, v. 46 n. 2, p. 95-102en_HK
dc.identifier.issn0732-8893en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79013-
dc.description.abstractPhenotypic identification of fungi in clinical microbiology laboratoreis is often difficult and late, especially for slow growing and rarely encountered fungi. We describe the application of 18S ribosomal RNA (rRNA) gene sequencing in the early diagnosis of a case of Exophiala peritonitis. A yeast-like fungus was isolated from the dialysate fluid of a 66-year-old man undergoing continuous ambulatory peritoneal dialysis. It grew slowly after 12 days of incubation to yield mature cultures to permit recognition of microscopic features resembling those of Exophiala, a dematiacerous mold. 18S rRNA gene sequencing provided results 12 days earlier than phenotypic identification and revealed 15 base difference (0.9%) between the isolate and Exophiala sp. strain GHP 1205 (GenBank Accession no. AJ232954), indicating that the isolate most closely resembles a strain of Exophiala species. The patient responded to 4 weeks of intravenous amphotericin B therapy. Early identification of the fungus was important for the choice of anti-fungal regimen. As opportunistic fungal infections in immunocompromised patients are globally emerging problems, the development of molecular techniques for fungal identification is crucial for early diagnosis and appropriate treatment. © 2003 Elsevier Science Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobioen_HK
dc.relation.ispartofDiagnostic Microbiology and Infectious Diseaseen_HK
dc.rightsDiagnostic Microbiology and Infectious Disease. Copyright © Elsevier Inc.en_HK
dc.subject.meshAgeden_HK
dc.subject.meshAmphotericin B - administration & dosageen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshDialysis Solutionsen_HK
dc.subject.meshExophiala - drug effects - isolation & purificationen_HK
dc.subject.meshGenes, rRNA - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKidney Failure, Chronic - diagnosis - therapyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshMycoses - diagnosis - drug therapyen_HK
dc.subject.meshPeritoneal Dialysis, Continuous Ambulatory - adverse effectsen_HK
dc.subject.meshPeritonitis - diagnosis - drug therapy - etiologyen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshRNA, Ribosomal, 18S - analysis - geneticsen_HK
dc.subject.meshRisk Assessmenten_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshTreatment Outcomeen_HK
dc.titleEarly diagnosis of Exophiala CAPD peritonitis by 18S ribosomal RNA gene sequencing and its clinical significanceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0732-8893&volume=46&spage=95&epage=102&date=2003&atitle=Early+diagnosis+of+exophiala+CAPD+peritonitis+by+18S+ribosomal+RNA+gene+sequencing+and+its+clinical+significanceen_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0732-8893(03)00014-2en_HK
dc.identifier.pmid12812724-
dc.identifier.scopuseid_2-s2.0-0038172618en_HK
dc.identifier.hkuros80124en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038172618&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue2en_HK
dc.identifier.spage95en_HK
dc.identifier.epage102en_HK
dc.identifier.isiWOS:000183651500003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridChiu, SK=7202291644en_HK
dc.identifier.scopusauthoridLeung, KW=7401860831en_HK
dc.identifier.scopusauthoridYung, RWH=7005594277en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats