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Article: Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments

TitleCharacterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments
Authors
KeywordsCoronavirus
DNA vaccine
Neutralizing antibody
SARS
Spike glycoprotein
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2004, v. 315 n. 4, p. 1134-1139 How to Cite?
AbstractThe immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18-495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV. © 2004 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78992
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZeng, Fen_HK
dc.contributor.authorChow, KYCen_HK
dc.contributor.authorHon, CCen_HK
dc.contributor.authorLaw, KMen_HK
dc.contributor.authorYip, CWen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorLeung, FCCen_HK
dc.date.accessioned2010-09-06T07:49:17Z-
dc.date.available2010-09-06T07:49:17Z-
dc.date.issued2004en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2004, v. 315 n. 4, p. 1134-1139en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/78992-
dc.description.abstractThe immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18-495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV. © 2004 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectCoronavirusen_HK
dc.subjectDNA vaccineen_HK
dc.subjectNeutralizing antibodyen_HK
dc.subjectSARSen_HK
dc.subjectSpike glycoproteinen_HK
dc.titleCharacterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragmentsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=315&spage=1134&epage=1139&date=2004&atitle=Characterization+of+humoral+responses+in+mice+immunized+with+plasmid+DNAs+encoding+SARS-CoV+spike+gene+fragmentsen_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2004.01.166en_HK
dc.identifier.pmid14985131-
dc.identifier.scopuseid_2-s2.0-1342324072en_HK
dc.identifier.hkuros85670en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1342324072&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume315en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1134en_HK
dc.identifier.epage1139en_HK
dc.identifier.isiWOS:000220105600053-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZeng, F=7202911544en_HK
dc.identifier.scopusauthoridChow, KYC=7202180875en_HK
dc.identifier.scopusauthoridHon, CC=7003617137en_HK
dc.identifier.scopusauthoridLaw, KM=7202563042en_HK
dc.identifier.scopusauthoridYip, CW=7101665559en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridLeung, FCC=7103078633en_HK

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