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Article: Performance evaluation of five detection tests for avian influenza antigen with various avian samples

TitlePerformance evaluation of five detection tests for avian influenza antigen with various avian samples
Authors
KeywordsAntigen test
ELISA
H5N1 avian influenza
Immunochromatographic test
Poultry
Waterfowl
Issue Date2007
PublisherAmerican Association of Avian Pathologists, Inc. The Journal's web site is located at http://avdi.allenpress.com/avdionline/?request=index-html
Citation
Avian Diseases, 2007, v. 51 n. 1, p. 96-105 How to Cite?
AbstractIn this paper, we report on the evaluation of five influenza antigen detection tests by avian influenza H5N1 virus-positive swab samples to estimate their diagnostic sensitivity. The tests included two chromatographic immunoassays, an H5 avian influenza-specific antigen detection enzyme-linked immunosorbent assay (ELISA), an influenza Aantigen detection ELISA, and an H5 rapid immunoblot assay. The results showed that the overall sensitivities of these tests ranged from 36.3% to 51.4% (95% confidence interval ranging from 31.0% to 57.0%), which were comparable to Directigen™ Flu A antigen detection tests but substantially lower than genome detection methods. Diagnostic sensitivity performance is a function of the concentration of antigens in samples and the analytical sensitivity of the individual test. The test sensitivities were significantly higher for sick and dead birds by cloacal, tracheal, or tissue swabs than for fecal swabs from apparently healthy birds, and these tests would not be suitable for surveillance testing of clinically healthy birds. Furthermore, the sensitivity for testing tracheal and cloacal swabs from waterfowl and wild birds was not as good as for chickens. This was most likely to be associated with variation in virus titers between specimens from different bird species. However, the tests showed good sensitivities for testing brain swabs from clinically affected waterfowl species. The results indicate that these antigen detection tests could be used for preliminary investigations of H5N1 outbreaks as a low-cost, simple flock test in sick and dead birds for the rapid detection of H5N1 infection. However, the relatively low sensitivity of the tests as individual bird tests means that they should be used on optimal clinical specimens from diseased birds, testing birds on a flock basis, or testing samples as close to the onset of disease as possible before viral titers diminish. They should be followed up by confirmatory tests, such as reverse transcription polymerase chain reaction or viral culture, wherever possible but could assist in facilitating rapid investigations and control interventions.
Persistent Identifierhttp://hdl.handle.net/10722/78988
ISSN
2014 Impact Factor: 1.241
2014 SCImago Journal Rankings: 0.644
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChua, THen_HK
dc.contributor.authorEllis, TMen_HK
dc.contributor.authorWong, CWen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorSheng, XGen_HK
dc.contributor.authorPeng, Gen_HK
dc.contributor.authorLamichhane, Cen_HK
dc.contributor.authorMaliadis, Cen_HK
dc.contributor.authorTan, SWen_HK
dc.contributor.authorSelleck, Pen_HK
dc.contributor.authorParkinson, Jen_HK
dc.date.accessioned2010-09-06T07:49:14Z-
dc.date.available2010-09-06T07:49:14Z-
dc.date.issued2007en_HK
dc.identifier.citationAvian Diseases, 2007, v. 51 n. 1, p. 96-105en_HK
dc.identifier.issn0005-2086en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78988-
dc.description.abstractIn this paper, we report on the evaluation of five influenza antigen detection tests by avian influenza H5N1 virus-positive swab samples to estimate their diagnostic sensitivity. The tests included two chromatographic immunoassays, an H5 avian influenza-specific antigen detection enzyme-linked immunosorbent assay (ELISA), an influenza Aantigen detection ELISA, and an H5 rapid immunoblot assay. The results showed that the overall sensitivities of these tests ranged from 36.3% to 51.4% (95% confidence interval ranging from 31.0% to 57.0%), which were comparable to Directigen™ Flu A antigen detection tests but substantially lower than genome detection methods. Diagnostic sensitivity performance is a function of the concentration of antigens in samples and the analytical sensitivity of the individual test. The test sensitivities were significantly higher for sick and dead birds by cloacal, tracheal, or tissue swabs than for fecal swabs from apparently healthy birds, and these tests would not be suitable for surveillance testing of clinically healthy birds. Furthermore, the sensitivity for testing tracheal and cloacal swabs from waterfowl and wild birds was not as good as for chickens. This was most likely to be associated with variation in virus titers between specimens from different bird species. However, the tests showed good sensitivities for testing brain swabs from clinically affected waterfowl species. The results indicate that these antigen detection tests could be used for preliminary investigations of H5N1 outbreaks as a low-cost, simple flock test in sick and dead birds for the rapid detection of H5N1 infection. However, the relatively low sensitivity of the tests as individual bird tests means that they should be used on optimal clinical specimens from diseased birds, testing birds on a flock basis, or testing samples as close to the onset of disease as possible before viral titers diminish. They should be followed up by confirmatory tests, such as reverse transcription polymerase chain reaction or viral culture, wherever possible but could assist in facilitating rapid investigations and control interventions.en_HK
dc.languageengen_HK
dc.publisherAmerican Association of Avian Pathologists, Inc. The Journal's web site is located at http://avdi.allenpress.com/avdionline/?request=index-htmlen_HK
dc.relation.ispartofAvian Diseasesen_HK
dc.subjectAntigen testen_HK
dc.subjectELISAen_HK
dc.subjectH5N1 avian influenzaen_HK
dc.subjectImmunochromatographic testen_HK
dc.subjectPoultryen_HK
dc.subjectWaterfowlen_HK
dc.titlePerformance evaluation of five detection tests for avian influenza antigen with various avian samplesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0005-2086&volume=51&issue=1&spage=96&epage=105&date=2007&atitle=Performance+evaluation+of+five+detection+tests+for+avian+influenza+antigen+with+various+avian+samples.en_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1637/0005-2086(2007)051[0096:PEOFDT]2.0.CO;2en_HK
dc.identifier.pmid17461273en_HK
dc.identifier.scopuseid_2-s2.0-33947602466en_HK
dc.identifier.hkuros138142en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33947602466&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume51en_HK
dc.identifier.issue1en_HK
dc.identifier.spage96en_HK
dc.identifier.epage105en_HK
dc.identifier.isiWOS:000245372100015-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChua, TH=53873610500en_HK
dc.identifier.scopusauthoridEllis, TM=7202589926en_HK
dc.identifier.scopusauthoridWong, CW=16067451100en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridSheng, XG=53874282300en_HK
dc.identifier.scopusauthoridPeng, G=53874094000en_HK
dc.identifier.scopusauthoridLamichhane, C=6603104782en_HK
dc.identifier.scopusauthoridMaliadis, C=53874068600en_HK
dc.identifier.scopusauthoridTan, SW=7403365796en_HK
dc.identifier.scopusauthoridSelleck, P=6701547852en_HK
dc.identifier.scopusauthoridParkinson, J=7102394972en_HK

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