Article: Streptococcus sinensis may react with Lancefield group F antiserum

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TitleStreptococcus sinensis may react with Lancefield group F antiserum
AuthorsWoo, PCY1
Teng, JLL1
Leung, KW1
Lau, SKP1
Tse, H1
Wong, BHL1
Yuen, KY1
Issue Date2004
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
CitationJournal Of Medical Microbiology, 2004, v. 53 n. 11, p. 1083-1088 [How to Cite?]
DOI: http://dx.doi.org/10.1099/jmm.0.45745-0
AbstractLancefield group F streptococci have been found almost exclusively as members of the 'Streptococcus miller' group, although they have been reported very occasionally in some other streptococcal species. Among 302 patients with bacteraemia caused by viridans streptococci over a 6-year period, three cases were caused by Streptococcus sinensis (type strain HKU4T, HKU5 and HKU6). All three patients had infective endocarditis complicating their underlying chronic rheumatic heart diseases. Gene sequencing showed no base differences between the 16S rRNA gene sequences of HKU5 and HKU6 and that of HKU4T. All three strains were Gram-positive, non-spore-forming cocci arranged in chains. All grew on sheep blood agar as α-haemolytic, grey colonies of 0·5-1 mm in diameter after 24 h incubation at 37 °C in ambient air. Lancefield grouping revealed that HKU5 and HKU6 were Lancefield group F, but HKU4T was non-groupable with Lancefield groups A, B, C, D, F or G antisera. HKU4T was identified by the Vitek system (GPI), API system (20 STREP) and ATB system (ID32 STREP) as 99% Streptococcus intermedius, 51·3% S. intermedius and 99·9% Streptococcus anginosus, respectively. Using the same tests, HKU5 was identified as 87% Streptococcus sanguinis/Streptococcus gordonii, 59% Streptococcus salivarius and 99·6% S. anginosus, respectively, and HKU6 as 87% S. sanguinis/S. gordonii, 77% Streptococcus pneumoniae and 98-3% S. anginosus, respectively. The present data revealed that a proportion of Lancefield group F streptococci could be S. sinensis. Lancefield group F streptococci should not be automatically reported as 'S. miller'.
ISSN0022-2615
2011 Impact Factor: 2.502
2011 SCImago Journal Rankings: 0.222
DOIhttp://dx.doi.org/10.1099/jmm.0.45745-0
ISI Accession Number IDWOS:000225112500005
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWoo, PCY
dc.contributor.authorTeng, JLL
dc.contributor.authorLeung, KW
dc.contributor.authorLau, SKP
dc.contributor.authorTse, H
dc.contributor.authorWong, BHL
dc.contributor.authorYuen, KY
dc.date.accessioned2010-09-06T07:49:10Z
dc.date.available2010-09-06T07:49:10Z
dc.date.issued2004
dc.description.abstractLancefield group F streptococci have been found almost exclusively as members of the 'Streptococcus miller' group, although they have been reported very occasionally in some other streptococcal species. Among 302 patients with bacteraemia caused by viridans streptococci over a 6-year period, three cases were caused by Streptococcus sinensis (type strain HKU4T, HKU5 and HKU6). All three patients had infective endocarditis complicating their underlying chronic rheumatic heart diseases. Gene sequencing showed no base differences between the 16S rRNA gene sequences of HKU5 and HKU6 and that of HKU4T. All three strains were Gram-positive, non-spore-forming cocci arranged in chains. All grew on sheep blood agar as α-haemolytic, grey colonies of 0·5-1 mm in diameter after 24 h incubation at 37 °C in ambient air. Lancefield grouping revealed that HKU5 and HKU6 were Lancefield group F, but HKU4T was non-groupable with Lancefield groups A, B, C, D, F or G antisera. HKU4T was identified by the Vitek system (GPI), API system (20 STREP) and ATB system (ID32 STREP) as 99% Streptococcus intermedius, 51·3% S. intermedius and 99·9% Streptococcus anginosus, respectively. Using the same tests, HKU5 was identified as 87% Streptococcus sanguinis/Streptococcus gordonii, 59% Streptococcus salivarius and 99·6% S. anginosus, respectively, and HKU6 as 87% S. sanguinis/S. gordonii, 77% Streptococcus pneumoniae and 98-3% S. anginosus, respectively. The present data revealed that a proportion of Lancefield group F streptococci could be S. sinensis. Lancefield group F streptococci should not be automatically reported as 'S. miller'.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Medical Microbiology, 2004, v. 53 n. 11, p. 1083-1088 [How to Cite?]
DOI: http://dx.doi.org/10.1099/jmm.0.45745-0
dc.identifier.doihttp://dx.doi.org/10.1099/jmm.0.45745-0
dc.identifier.epage1088
dc.identifier.hkuros100177
dc.identifier.isiWOS:000225112500005
dc.identifier.issn0022-2615
2011 Impact Factor: 2.502
2011 SCImago Journal Rankings: 0.222
dc.identifier.issue11
dc.identifier.openurl
dc.identifier.pmid15496384
dc.identifier.scopuseid_2-s2.0-11144224250
dc.identifier.spage1083
dc.identifier.urihttp://hdl.handle.net/10722/78983
dc.identifier.volume53
dc.languageeng
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
dc.publisher.placeUnited Kingdom
dc.relation.ispartofJournal of Medical Microbiology
dc.relation.referencesReferences in Scopus
dc.subject.meshBacteremia - microbiology
dc.subject.meshDNA, Bacterial - chemistry - isolation & purification
dc.subject.meshDNA, Ribosomal - chemistry - isolation & purification
dc.subject.meshEndocarditis, Bacterial - microbiology
dc.subject.meshGenes, rRNA - genetics
dc.subject.meshGentian Violet
dc.subject.meshGram-Positive Cocci
dc.subject.meshHemolysis
dc.subject.meshHumans
dc.subject.meshMolecular Sequence Data
dc.subject.meshPhenazines
dc.subject.meshPhylogeny
dc.subject.meshRNA, Bacterial - genetics
dc.subject.meshRNA, Ribosomal, 16S - genetics
dc.subject.meshSequence Analysis, DNA
dc.subject.meshSerotyping
dc.subject.meshSpores, Bacterial
dc.subject.meshStreptococcal Infections - diagnosis - microbiology
dc.subject.meshViridans Streptococci - classification - cytology - immunology - physiology
dc.titleStreptococcus sinensis may react with Lancefield group F antiserum
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong