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Article: Characterization of a novel cryptic plasmid, pHLHK26, in Laribacter hongkongensis

TitleCharacterization of a novel cryptic plasmid, pHLHK26, in Laribacter hongkongensis
Authors
Issue Date2007
PublisherEdizioni Medico Scientifiche (E D I M E S). The Journal's web site is located at http://www.microbiologica.net
Citation
New Microbiologica, 2007, v. 30 n. 2, p. 139-147 How to Cite?
AbstractWe report the complete nucleotide sequence and characterization of a cryptic plasmid, pHLHK26, recovered from a strain of Laribacter hongkongensis isolated from a patient with community acquired gastroenteritis. pHLHK26 consists of 8700 bp, with G + C content 51.3%. The copy number (mean±SD) is 0.57±0.07 and it is stable after four passages (about 240 generations) in the absence of selection. There is a predicted origin of replication that consists of a DnaA box and five 22-bp direct repeats. pHLHK26 has four ORFs with two genes encoded in the sense direction and the other two in antisense direction. These four ORFs encode a putative plasmid partitioning protein of the ParA family, a putative protein that contains putative ADP-ribose 1"-phosphatase activity belonging to the Appr-1 -p processing enzyme family, a putative recombinase (TniR) of the resolvase/invertase family, and a putative replication protein, respectively. We speculate that pHLHK26 is a theta, possibly Class A, replicative plasmid, as it contains an origin of replication with AT-rich region, a number of herons and a DnaA box and a gene that encodes a replicative protein most homologous to those of other theta replicative plasmids and it shares eight of the nine positions of the consensus sequence TTAT(C/A)CA(C/A)A (TTTTCCACA in pHLHK26) in the DnaA boxes observed in other classical examples of Class A plasmids of this group.
Persistent Identifierhttp://hdl.handle.net/10722/78947
ISSN
2015 Impact Factor: 1.629
2015 SCImago Journal Rankings: 0.786
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorTeng, JLLen_HK
dc.contributor.authorMa, SSLen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:48:44Z-
dc.date.available2010-09-06T07:48:44Z-
dc.date.issued2007en_HK
dc.identifier.citationNew Microbiologica, 2007, v. 30 n. 2, p. 139-147en_HK
dc.identifier.issn1121-7138en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78947-
dc.description.abstractWe report the complete nucleotide sequence and characterization of a cryptic plasmid, pHLHK26, recovered from a strain of Laribacter hongkongensis isolated from a patient with community acquired gastroenteritis. pHLHK26 consists of 8700 bp, with G + C content 51.3%. The copy number (mean±SD) is 0.57±0.07 and it is stable after four passages (about 240 generations) in the absence of selection. There is a predicted origin of replication that consists of a DnaA box and five 22-bp direct repeats. pHLHK26 has four ORFs with two genes encoded in the sense direction and the other two in antisense direction. These four ORFs encode a putative plasmid partitioning protein of the ParA family, a putative protein that contains putative ADP-ribose 1"-phosphatase activity belonging to the Appr-1 -p processing enzyme family, a putative recombinase (TniR) of the resolvase/invertase family, and a putative replication protein, respectively. We speculate that pHLHK26 is a theta, possibly Class A, replicative plasmid, as it contains an origin of replication with AT-rich region, a number of herons and a DnaA box and a gene that encodes a replicative protein most homologous to those of other theta replicative plasmids and it shares eight of the nine positions of the consensus sequence TTAT(C/A)CA(C/A)A (TTTTCCACA in pHLHK26) in the DnaA boxes observed in other classical examples of Class A plasmids of this group.en_HK
dc.languageengen_HK
dc.publisherEdizioni Medico Scientifiche (E D I M E S). The Journal's web site is located at http://www.microbiologica.neten_HK
dc.relation.ispartofNew Microbiologicaen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshBacterial Proteins - genetics - metabolismen_HK
dc.subject.meshBase Compositionen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBinding Sites - geneticsen_HK
dc.subject.meshCommunity-Acquired Infections - microbiologyen_HK
dc.subject.meshConsensus Sequenceen_HK
dc.subject.meshDNA Helicases - geneticsen_HK
dc.subject.meshDNA, Bacterial - chemistry - geneticsen_HK
dc.subject.meshDNA-Binding Proteins - genetics - metabolismen_HK
dc.subject.meshGastroenteritis - microbiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNeisseriaceae - genetics - isolation & purificationen_HK
dc.subject.meshOpen Reading Framesen_HK
dc.subject.meshPhosphoric Monoester Hydrolases - geneticsen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshPlasmids - geneticsen_HK
dc.subject.meshRecombinases - geneticsen_HK
dc.subject.meshReplication Origin - geneticsen_HK
dc.subject.meshSequence Alignmenten_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshTrans-Activators - geneticsen_HK
dc.titleCharacterization of a novel cryptic plasmid, pHLHK26, in Laribacter hongkongensisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1121-7138&volume=30&spage=139&epage=146&date=2007&atitle=Characterization+of+a+novel+cryptic+plasmid,+pHLHK26,+in+Laribacter+hongkongensisen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTeng, JLL:llteng@hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTeng, JLL=rp00277en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid17619258-
dc.identifier.scopuseid_2-s2.0-34250863337en_HK
dc.identifier.hkuros127779en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34250863337&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue2en_HK
dc.identifier.spage139en_HK
dc.identifier.epage147en_HK
dc.identifier.isiWOS:000247405600010-
dc.publisher.placeItalyen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridTeng, JLL=7202560229en_HK
dc.identifier.scopusauthoridMa, SSL=26424668200en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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