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Article: Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans

TitleHeterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans
Authors
Issue Date2008
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/
Citation
Virology Journal, 2008, v. 5 How to Cite?
AbstractThe initiation of transcription and replication of influenza A virus requires the 5′ and 3′ ends of vRNA. Here, the role of segment-specific non-coding sequences of influenza A virus on viral RNA synthesis was studied. Recombinant viruses, with the nonstructural protein (NS) segment-specific non-coding sequences replaced by the corresponding sequences of the neuraminidase (NA) segment, were characterized. The NS and NA vRNA levels in cells infected with these mutants were much higher than those of the wild type, whereas the NS and NA mRNA levels of the mutants were comparable to the wild-type levels. By contrast, the PB2 vRNA and mRNA levels of all the tested viruses were similar, indicating that vRNA with heterologous segment-specific non-coding sequences was not affected by the mutations. The observations suggested that, with the cooperation between the homologous 5′ and 3′segment-specific sequences, the introduced mutations could specifically enhance the replication of NA and NS vRNA. © 2008 Ng et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/78935
ISSN
2015 Impact Factor: 2.362
2015 SCImago Journal Rankings: 1.166
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, SSFen_HK
dc.contributor.authorLi, OTWen_HK
dc.contributor.authorCheung, TKWen_HK
dc.contributor.authorMalik Peiris, JSen_HK
dc.contributor.authorPoon, LLMen_HK
dc.date.accessioned2010-09-06T07:48:35Z-
dc.date.available2010-09-06T07:48:35Z-
dc.date.issued2008en_HK
dc.identifier.citationVirology Journal, 2008, v. 5en_HK
dc.identifier.issn1743-422Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/78935-
dc.description.abstractThe initiation of transcription and replication of influenza A virus requires the 5′ and 3′ ends of vRNA. Here, the role of segment-specific non-coding sequences of influenza A virus on viral RNA synthesis was studied. Recombinant viruses, with the nonstructural protein (NS) segment-specific non-coding sequences replaced by the corresponding sequences of the neuraminidase (NA) segment, were characterized. The NS and NA vRNA levels in cells infected with these mutants were much higher than those of the wild type, whereas the NS and NA mRNA levels of the mutants were comparable to the wild-type levels. By contrast, the PB2 vRNA and mRNA levels of all the tested viruses were similar, indicating that vRNA with heterologous segment-specific non-coding sequences was not affected by the mutations. The observations suggested that, with the cooperation between the homologous 5′ and 3′segment-specific sequences, the introduced mutations could specifically enhance the replication of NA and NS vRNA. © 2008 Ng et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_HK
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/en_HK
dc.relation.ispartofVirology Journalen_HK
dc.rightsVirology Journal. Copyright © BioMed Central Ltd.en_HK
dc.titleHeterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in transen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1743-422X&volume=5&spage=2&epage=&date=2008&atitle=Heterologous+influenza+vRNA+segments+with+identical+non-coding+sequences+stimulate+viral+RNA+replication+in+trans.en_HK
dc.identifier.emailMalik Peiris, JS: malik@hkucc.hku.hken_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.authorityMalik Peiris, JS=rp00410en_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1186/1743-422X-5-2en_HK
dc.identifier.pmid18186945-
dc.identifier.scopuseid_2-s2.0-40449116417en_HK
dc.identifier.hkuros149326en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40449116417&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.isiWOS:000253793200001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridNg, SSF=8602085800en_HK
dc.identifier.scopusauthoridLi, OTW=16230718400en_HK
dc.identifier.scopusauthoridCheung, TKW=16229531100en_HK
dc.identifier.scopusauthoridMalik Peiris, JS=7005486823en_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.citeulike2221123-

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