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Article: Characterization of the pathogenicity of members of the newly established H9N2 influenza virus lineages in Asia

TitleCharacterization of the pathogenicity of members of the newly established H9N2 influenza virus lineages in Asia
Authors
Issue Date2000
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 2000, v. 267 n. 2, p. 279-288 How to Cite?
AbstractThe reported transmission of avian H9N2 influenza viruses to humans and the isolation of these viruses from Hong Kong poultry markets lend urgency to studies of their ecology and pathogenicity. We found that H9N2 viruses from North America differ from those of Asia. The North American viruses, which infect primarily domestic turkeys, replicated poorly in inoculated chickens. Phylogenetic analysis of the hemagglutinin and nucleoprotein genes indicated that the Asian H9N2 influenza viruses could be divided into three sublineages. Initial biological characterization of at least one virus from each lineage was done in animals. Early isolates of one lineage (A/Chicken/Beijing/1/94, H9N2) caused as high as 80% mortality rates in inoculated chickens, whereas all other strains were nonpathogenic. Sequence analysis showed that some isolates, including the pathogenic isolate, had one additional basic amino acid (A-R/K-S-S-R-) at the hemagglutinin cleavage site. Later isolates of the same lineage (A/Chicken/Hong Kong/G9/97, H9N2) that contains the PB1 and PB2 genes similar to Hong Kong/97 H5N1 viruses replicated in chickens, ducks, mice, and pigs but were pathogenic only in mice. A/Quail/Hong Kong/G1/97 (H9N2), from a second lineage that possesses the replicative complex similar to Hong Kong/97 HSN1 virus; replicated in chickens and ducks without producing disease signs, was pathogenic in mice, and spread to the brain without adaptation. Examples of the third Asian H9N2 sublineage (A/Chicken/Korea/323/96, Duck/Hong Kong/Y439/97) replicated in chickens, ducks, and mice without producing disease signs. The available evidence supports the notion of differences in pathogenicity of H9N2 viruses in the different lineages and suggests that viruses possessing genome segments similar to 1997 H5N1-like viruses are potentially pathogenic in mammals. (C) 2000 Academic Press.
Persistent Identifierhttp://hdl.handle.net/10722/78901
ISSN
2015 Impact Factor: 3.2
2015 SCImago Journal Rankings: 1.805
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuo, YJen_HK
dc.contributor.authorKrauss, Sen_HK
dc.contributor.authorSenne, DAen_HK
dc.contributor.authorMo, IPen_HK
dc.contributor.authorLo, KSen_HK
dc.contributor.authorXiong, XPen_HK
dc.contributor.authorNorwood, Men_HK
dc.contributor.authorShortridge, KFen_HK
dc.contributor.authorWebster, RGen_HK
dc.contributor.authorGuan, Yen_HK
dc.date.accessioned2010-09-06T07:48:11Z-
dc.date.available2010-09-06T07:48:11Z-
dc.date.issued2000en_HK
dc.identifier.citationVirology, 2000, v. 267 n. 2, p. 279-288en_HK
dc.identifier.issn0042-6822en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78901-
dc.description.abstractThe reported transmission of avian H9N2 influenza viruses to humans and the isolation of these viruses from Hong Kong poultry markets lend urgency to studies of their ecology and pathogenicity. We found that H9N2 viruses from North America differ from those of Asia. The North American viruses, which infect primarily domestic turkeys, replicated poorly in inoculated chickens. Phylogenetic analysis of the hemagglutinin and nucleoprotein genes indicated that the Asian H9N2 influenza viruses could be divided into three sublineages. Initial biological characterization of at least one virus from each lineage was done in animals. Early isolates of one lineage (A/Chicken/Beijing/1/94, H9N2) caused as high as 80% mortality rates in inoculated chickens, whereas all other strains were nonpathogenic. Sequence analysis showed that some isolates, including the pathogenic isolate, had one additional basic amino acid (A-R/K-S-S-R-) at the hemagglutinin cleavage site. Later isolates of the same lineage (A/Chicken/Hong Kong/G9/97, H9N2) that contains the PB1 and PB2 genes similar to Hong Kong/97 H5N1 viruses replicated in chickens, ducks, mice, and pigs but were pathogenic only in mice. A/Quail/Hong Kong/G1/97 (H9N2), from a second lineage that possesses the replicative complex similar to Hong Kong/97 HSN1 virus; replicated in chickens and ducks without producing disease signs, was pathogenic in mice, and spread to the brain without adaptation. Examples of the third Asian H9N2 sublineage (A/Chicken/Korea/323/96, Duck/Hong Kong/Y439/97) replicated in chickens, ducks, and mice without producing disease signs. The available evidence supports the notion of differences in pathogenicity of H9N2 viruses in the different lineages and suggests that viruses possessing genome segments similar to 1997 H5N1-like viruses are potentially pathogenic in mammals. (C) 2000 Academic Press.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviroen_HK
dc.relation.ispartofVirologyen_HK
dc.titleCharacterization of the pathogenicity of members of the newly established H9N2 influenza virus lineages in Asiaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0042-6822&volume=267&spage=279&epage=288&date=2000&atitle=Characterization+of+the+pathogenicity+of+members+of+the+newly+established+H9N2+influenza+virus+lineages+in+Asiaen_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/viro.1999.0115en_HK
dc.identifier.pmid10662623-
dc.identifier.scopuseid_2-s2.0-18244426024en_HK
dc.identifier.hkuros54160en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18244426024&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume267en_HK
dc.identifier.issue2en_HK
dc.identifier.spage279en_HK
dc.identifier.epage288en_HK
dc.identifier.isiWOS:000085434000015-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGuo, YJ=7406311197en_HK
dc.identifier.scopusauthoridKrauss, S=7102769210en_HK
dc.identifier.scopusauthoridSenne, DA=7003722049en_HK
dc.identifier.scopusauthoridMo, IP=6603063774en_HK
dc.identifier.scopusauthoridLo, KS=21737269500en_HK
dc.identifier.scopusauthoridXiong, XP=7201634310en_HK
dc.identifier.scopusauthoridNorwood, M=36881225800en_HK
dc.identifier.scopusauthoridShortridge, KF=7005677034en_HK
dc.identifier.scopusauthoridWebster, RG=36048363100en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK

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