File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Estimation of bacterial risk in extending the shelf life of PLT concentrates from 5 to 7 days

TitleEstimation of bacterial risk in extending the shelf life of PLT concentrates from 5 to 7 days
Authors
Issue Date2003
PublisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/TRF
Citation
Transfusion, 2003, v. 43 n. 8, p. 1047-1052 How to Cite?
AbstractBACKGROUND: The use of bacterial culture to prevent bacterial contamination of blood components has renewed interest for extending the shelf life of PLT concentrates to 7 days after collection. STUDY DESIGN AND METHODS: This study was therefore conducted to determine the residual risk of bacterial contamination in PLT concentrates at the end of 5 and 7 days after collection in a center where all PLT concentrates are routinely screened by taking samples on Day 2 for culture. PLT units with no growth after 48 hours were sampled a second time on Day 5 or Day 7 after collection, followed by inoculation into aerobic culture bottles. The inoculated bottles were then monitored for up to 7 days at 35°C in an automatic monitoring and detection system. RESULTS: During a 16-month study period, a total of 6020 PLT concentrates were tested 5 days (Group A, n = 3010) and 7 days (Group B, n = 3010) after collection. Four units in each group (0.133%) were found to be contaminated. In 6 units, bacteria were seen on direct Gram stain. In addition, 5 of the associated RBC units grew the same organisms on culture. The organisms include three coagulase-negative staphylococci and five Propionibacterium acnes. The positive rate of routine short-term bacterial culture was 0.035 percent during the same study period. CONCLUSION: Despite routine short-term bacterial culture, a significant risk of bacterial contamination remains at 5 and 7 days after collection. For now, the shelf life of PLT concentrates should remain 5 days.
Persistent Identifierhttp://hdl.handle.net/10722/78897
ISSN
2015 Impact Factor: 3.042
2015 SCImago Journal Rankings: 1.537
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, CKen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorLee, KYen_HK
dc.contributor.authorCheng, WWen_HK
dc.contributor.authorChan, NKen_HK
dc.contributor.authorTsoi, WCen_HK
dc.contributor.authorLin, CKen_HK
dc.date.accessioned2010-09-06T07:48:08Z-
dc.date.available2010-09-06T07:48:08Z-
dc.date.issued2003en_HK
dc.identifier.citationTransfusion, 2003, v. 43 n. 8, p. 1047-1052en_HK
dc.identifier.issn0041-1132en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78897-
dc.description.abstractBACKGROUND: The use of bacterial culture to prevent bacterial contamination of blood components has renewed interest for extending the shelf life of PLT concentrates to 7 days after collection. STUDY DESIGN AND METHODS: This study was therefore conducted to determine the residual risk of bacterial contamination in PLT concentrates at the end of 5 and 7 days after collection in a center where all PLT concentrates are routinely screened by taking samples on Day 2 for culture. PLT units with no growth after 48 hours were sampled a second time on Day 5 or Day 7 after collection, followed by inoculation into aerobic culture bottles. The inoculated bottles were then monitored for up to 7 days at 35°C in an automatic monitoring and detection system. RESULTS: During a 16-month study period, a total of 6020 PLT concentrates were tested 5 days (Group A, n = 3010) and 7 days (Group B, n = 3010) after collection. Four units in each group (0.133%) were found to be contaminated. In 6 units, bacteria were seen on direct Gram stain. In addition, 5 of the associated RBC units grew the same organisms on culture. The organisms include three coagulase-negative staphylococci and five Propionibacterium acnes. The positive rate of routine short-term bacterial culture was 0.035 percent during the same study period. CONCLUSION: Despite routine short-term bacterial culture, a significant risk of bacterial contamination remains at 5 and 7 days after collection. For now, the shelf life of PLT concentrates should remain 5 days.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/TRFen_HK
dc.relation.ispartofTransfusionen_HK
dc.subject.meshBacteria - growth & developmenten_HK
dc.subject.meshBlood - microbiologyen_HK
dc.subject.meshBlood Platelets - microbiologyen_HK
dc.subject.meshBlood Preservationen_HK
dc.subject.meshColony Count, Microbialen_HK
dc.subject.meshHumansen_HK
dc.subject.meshRisk Factorsen_HK
dc.subject.meshTime Factorsen_HK
dc.titleEstimation of bacterial risk in extending the shelf life of PLT concentrates from 5 to 7 daysen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0041-1132&volume=43&issue=8&spage=1047&epage=52&date=2003&atitle=Estimation+of+bacterial+risk+in+extending+the+shelf+life+of+PLT+concentrates+from+5+to+7+days.en_HK
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1537-2995.2003.00456.xen_HK
dc.identifier.pmid12869109-
dc.identifier.scopuseid_2-s2.0-0042931310en_HK
dc.identifier.hkuros83448en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0042931310&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1047en_HK
dc.identifier.epage1052en_HK
dc.identifier.isiWOS:000184418100010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, CK=36087620900en_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridLee, KY=7501500790en_HK
dc.identifier.scopusauthoridCheng, WW=7402169359en_HK
dc.identifier.scopusauthoridChan, NK=7101677541en_HK
dc.identifier.scopusauthoridTsoi, WC=36852604400en_HK
dc.identifier.scopusauthoridLin, CK=12752556900en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats