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Article: Diagnosis of cytomegalovirus (CMV) disease in renal allograft recipients: The role of semiquantitative polymerase chain reaction (PCR)

TitleDiagnosis of cytomegalovirus (CMV) disease in renal allograft recipients: The role of semiquantitative polymerase chain reaction (PCR)
Authors
KeywordsCytomegalovirus
DEAFF test
Ganciclovir
Immunocompromised
PCR
Renal transplant
Serology
Shell vial culture
Issue Date1995
PublisherOxford University Press. The Journal's web site is located at http://ndt.oxfordjournals.org/
Citation
Nephrology Dialysis Transplantation, 1995, v. 10 n. 7, p. 1198-1205 How to Cite?
AbstractBackground Cytomegalovirus disease remains a significant cause of morbidity and mortality in the renal allograft recipient. There is a need for rapid and sensitive techniques predictive of CMV disease to allow initiation of early antiviral therapy. Methods. Seventy-seven renal allograft recipients were enrolled in a prospective study where CMV viruria (shell vial culture/DEAFF test), viraemia (shell vial culture), serology and detection of virus DNA in peripheral blood leukocytes by PCR (CMV DNAemia) were correlated with clinical evidence of CMV disease. Results. Serology and shell vial culture had poor sensitivity for the early diagnosis of CMV disease. CMV DNAemia appeared to correlate with active virus replication. CMV DNAemia had a sensitivity and negative predictive value of 100% and a positive predictive value of 27% for CMV disease. Patients with symptomatic CMV disease were shown to have higher levels of CMV DNAemia than those with asymptomatic infection. Conclusions. A negative CMV DNAemia result excluded CMV disease with confidence, but a positive result (given the low positive predictive value) did not by itself provide a reliable guide for the initiation of pre-emptive antiviral therapy. However, the semiquantitative CMV DNAemia result taken together with the clinical findings provided useful information for patient management.
Persistent Identifierhttp://hdl.handle.net/10722/78889
ISSN
2015 Impact Factor: 4.085
2015 SCImago Journal Rankings: 1.780
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorTaylor, CEen_HK
dc.contributor.authorMain, Jen_HK
dc.contributor.authorGraham, Ken_HK
dc.contributor.authorMadeley, CRen_HK
dc.date.accessioned2010-09-06T07:48:02Z-
dc.date.available2010-09-06T07:48:02Z-
dc.date.issued1995en_HK
dc.identifier.citationNephrology Dialysis Transplantation, 1995, v. 10 n. 7, p. 1198-1205en_HK
dc.identifier.issn0931-0509en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78889-
dc.description.abstractBackground Cytomegalovirus disease remains a significant cause of morbidity and mortality in the renal allograft recipient. There is a need for rapid and sensitive techniques predictive of CMV disease to allow initiation of early antiviral therapy. Methods. Seventy-seven renal allograft recipients were enrolled in a prospective study where CMV viruria (shell vial culture/DEAFF test), viraemia (shell vial culture), serology and detection of virus DNA in peripheral blood leukocytes by PCR (CMV DNAemia) were correlated with clinical evidence of CMV disease. Results. Serology and shell vial culture had poor sensitivity for the early diagnosis of CMV disease. CMV DNAemia appeared to correlate with active virus replication. CMV DNAemia had a sensitivity and negative predictive value of 100% and a positive predictive value of 27% for CMV disease. Patients with symptomatic CMV disease were shown to have higher levels of CMV DNAemia than those with asymptomatic infection. Conclusions. A negative CMV DNAemia result excluded CMV disease with confidence, but a positive result (given the low positive predictive value) did not by itself provide a reliable guide for the initiation of pre-emptive antiviral therapy. However, the semiquantitative CMV DNAemia result taken together with the clinical findings provided useful information for patient management.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://ndt.oxfordjournals.org/en_HK
dc.relation.ispartofNephrology Dialysis Transplantationen_HK
dc.rightsNephrology, Dialysis, Transplantation. Copyright © Oxford University Press.en_HK
dc.subjectCytomegalovirusen_HK
dc.subjectDEAFF testen_HK
dc.subjectGancicloviren_HK
dc.subjectImmunocompromiseden_HK
dc.subjectPCRen_HK
dc.subjectRenal transplanten_HK
dc.subjectSerologyen_HK
dc.subjectShell vial cultureen_HK
dc.titleDiagnosis of cytomegalovirus (CMV) disease in renal allograft recipients: The role of semiquantitative polymerase chain reaction (PCR)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0931-0509&volume=10&spage=1198&epage=1205&date=1996&atitle=Diagnosis+of+cytomegalovirus+(CMV)+disease+in+renal+allograft+recipients:+the+role+of+semiquantitative+polymerase+chain+reaction+(PCR)en_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid7478124-
dc.identifier.scopuseid_2-s2.0-0029148260en_HK
dc.identifier.hkuros60499en_HK
dc.identifier.volume10en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1198en_HK
dc.identifier.epage1205en_HK
dc.identifier.isiWOS:A1995RM84300024-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridTaylor, CE=7404822545en_HK
dc.identifier.scopusauthoridMain, J=7103372157en_HK
dc.identifier.scopusauthoridGraham, K=55057455500en_HK
dc.identifier.scopusauthoridMadeley, CR=7006274504en_HK

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