Article: Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing

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TitleAnaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing
AuthorsLau, SKP1
Woo, PCY1
Fung, AMY1
Chan, KM1
Woo, GKS1
Yuen, KY1
Issue Date2004
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
CitationJournal Of Medical Microbiology, 2004, v. 53 n. 12, p. 1247-1253 [How to Cite?]
DOI: http://dx.doi.org/10.1099/jmm.0.45803-0
AbstractOwing to the difficulties in identifying anaerobic, non-sporulating, Gram-positive bacilli in clinical microbiology laboratories, the epidemiology and clinical spectrum of disease of many of these bacteria have been poorly understood. The application of 16S rRNA gene sequencing in characterizing bacteraemia due to anaerobic, non-sporulating Gram-positive bacilli during a 4-year period is described. The first case of Olsenella uli bacteraemia, in a patient with acute cholangitis, is also reported. Among 165 blood culture isolates of anaerobic, Gram-positive bacilli, 75 were identified as Propionibacterium acnes by phenotypic tests and 21 as members of other anaerobic, non-sporulating Gram-positive bacilli by 16S rRNA gene sequencing. Of these 96 isolates, 16 (17 %) were associated with cases of clinically significant bacteraemia, among which 10 (63 %) were caused by Eggerthella, four (25 %) by Lactobacillus and one (6 %) by each of Eubacterium tenue and O. uli. Five of the 10 Eggerthella isolates were Eggerthella lenta, whereas the other five belonged to two novel Eggerthella species, with Eggerthella hongkongensis being almost as prevalent as Eggerthella lenta. Underlying disease in the gastrointestinal tract, isolation of Eggerthella and Lactobacillus, and monomicrobial bacteraemia were associated with clinically significant bacteraemia, whereas isolation of P. acnes and polymicrobial bacteraemia were associated with pseudobacteraemia. Most patients with clinically significant bacteraemia had underlying diseases, with diseases in the gastrointestinal tract being most common. The overall mortality rate was 31 %. Immunocompromised patients with clinically significant bacteraemia due to anaerobic, non-sporulating, Gram-positive bacilli other than P. acnes should be treated with appropriate antibiotics. The unexpected frequency of isolation of Eggerthella from blood cultures and its association with clinically significant disease suggest that this genus is probably of high pathogenicity. Further studies to look for specific virulence factors are warranted.
ISSN0022-2615
2011 Impact Factor: 2.502
2011 SCImago Journal Rankings: 0.222
DOIhttp://dx.doi.org/10.1099/jmm.0.45803-0
ISI Accession Number IDWOS:000225893300012
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLau, SKP
dc.contributor.authorWoo, PCY
dc.contributor.authorFung, AMY
dc.contributor.authorChan, KM
dc.contributor.authorWoo, GKS
dc.contributor.authorYuen, KY
dc.date.accessioned2010-09-06T07:47:54Z
dc.date.available2010-09-06T07:47:54Z
dc.date.issued2004
dc.description.abstractOwing to the difficulties in identifying anaerobic, non-sporulating, Gram-positive bacilli in clinical microbiology laboratories, the epidemiology and clinical spectrum of disease of many of these bacteria have been poorly understood. The application of 16S rRNA gene sequencing in characterizing bacteraemia due to anaerobic, non-sporulating Gram-positive bacilli during a 4-year period is described. The first case of Olsenella uli bacteraemia, in a patient with acute cholangitis, is also reported. Among 165 blood culture isolates of anaerobic, Gram-positive bacilli, 75 were identified as Propionibacterium acnes by phenotypic tests and 21 as members of other anaerobic, non-sporulating Gram-positive bacilli by 16S rRNA gene sequencing. Of these 96 isolates, 16 (17 %) were associated with cases of clinically significant bacteraemia, among which 10 (63 %) were caused by Eggerthella, four (25 %) by Lactobacillus and one (6 %) by each of Eubacterium tenue and O. uli. Five of the 10 Eggerthella isolates were Eggerthella lenta, whereas the other five belonged to two novel Eggerthella species, with Eggerthella hongkongensis being almost as prevalent as Eggerthella lenta. Underlying disease in the gastrointestinal tract, isolation of Eggerthella and Lactobacillus, and monomicrobial bacteraemia were associated with clinically significant bacteraemia, whereas isolation of P. acnes and polymicrobial bacteraemia were associated with pseudobacteraemia. Most patients with clinically significant bacteraemia had underlying diseases, with diseases in the gastrointestinal tract being most common. The overall mortality rate was 31 %. Immunocompromised patients with clinically significant bacteraemia due to anaerobic, non-sporulating, Gram-positive bacilli other than P. acnes should be treated with appropriate antibiotics. The unexpected frequency of isolation of Eggerthella from blood cultures and its association with clinically significant disease suggest that this genus is probably of high pathogenicity. Further studies to look for specific virulence factors are warranted.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Medical Microbiology, 2004, v. 53 n. 12, p. 1247-1253 [How to Cite?]
DOI: http://dx.doi.org/10.1099/jmm.0.45803-0
dc.identifier.doihttp://dx.doi.org/10.1099/jmm.0.45803-0
dc.identifier.epage1253
dc.identifier.hkuros100211
dc.identifier.isiWOS:000225893300012
dc.identifier.issn0022-2615
2011 Impact Factor: 2.502
2011 SCImago Journal Rankings: 0.222
dc.identifier.issue12
dc.identifier.openurl
dc.identifier.pmid15585505
dc.identifier.scopuseid_2-s2.0-11144241375
dc.identifier.spage1247
dc.identifier.urihttp://hdl.handle.net/10722/78877
dc.identifier.volume53
dc.languageeng
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
dc.publisher.placeUnited Kingdom
dc.relation.ispartofJournal of Medical Microbiology
dc.relation.referencesReferences in Scopus
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshAged, 80 and over
dc.subject.meshBacteremia - diagnosis - microbiology
dc.subject.meshBacteria, Anaerobic - classification - isolation & purification
dc.subject.meshBacterial Typing Techniques - methods
dc.subject.meshFemale
dc.subject.meshGram-Positive Bacteria - classification - isolation & purification
dc.subject.meshGram-Positive Bacterial Infections - diagnosis - epidemiology - microbiology
dc.subject.meshHong Kong - epidemiology
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshMolecular Epidemiology
dc.subject.meshRNA, Bacterial - genetics
dc.subject.meshRNA, Ribosomal, 16S - genetics
dc.subject.meshSpecies Specificity
dc.titleAnaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong