File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing
  • Basic View
  • Metadata View
  • XML View
TitleAnaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing
 
AuthorsLau, SKP1
Woo, PCY1
Fung, AMY1
Chan, KM1
Woo, GKS1
Yuen, KY1
 
Issue Date2004
 
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
 
CitationJournal Of Medical Microbiology, 2004, v. 53 n. 12, p. 1247-1253 [How to Cite?]
DOI: http://dx.doi.org/10.1099/jmm.0.45803-0
 
AbstractOwing to the difficulties in identifying anaerobic, non-sporulating, Gram-positive bacilli in clinical microbiology laboratories, the epidemiology and clinical spectrum of disease of many of these bacteria have been poorly understood. The application of 16S rRNA gene sequencing in characterizing bacteraemia due to anaerobic, non-sporulating Gram-positive bacilli during a 4-year period is described. The first case of Olsenella uli bacteraemia, in a patient with acute cholangitis, is also reported. Among 165 blood culture isolates of anaerobic, Gram-positive bacilli, 75 were identified as Propionibacterium acnes by phenotypic tests and 21 as members of other anaerobic, non-sporulating Gram-positive bacilli by 16S rRNA gene sequencing. Of these 96 isolates, 16 (17 %) were associated with cases of clinically significant bacteraemia, among which 10 (63 %) were caused by Eggerthella, four (25 %) by Lactobacillus and one (6 %) by each of Eubacterium tenue and O. uli. Five of the 10 Eggerthella isolates were Eggerthella lenta, whereas the other five belonged to two novel Eggerthella species, with Eggerthella hongkongensis being almost as prevalent as Eggerthella lenta. Underlying disease in the gastrointestinal tract, isolation of Eggerthella and Lactobacillus, and monomicrobial bacteraemia were associated with clinically significant bacteraemia, whereas isolation of P. acnes and polymicrobial bacteraemia were associated with pseudobacteraemia. Most patients with clinically significant bacteraemia had underlying diseases, with diseases in the gastrointestinal tract being most common. The overall mortality rate was 31 %. Immunocompromised patients with clinically significant bacteraemia due to anaerobic, non-sporulating, Gram-positive bacilli other than P. acnes should be treated with appropriate antibiotics. The unexpected frequency of isolation of Eggerthella from blood cultures and its association with clinically significant disease suggest that this genus is probably of high pathogenicity. Further studies to look for specific virulence factors are warranted.
 
ISSN0022-2615
2013 Impact Factor: 2.266
2013 SCImago Journal Rankings: 1.025
 
DOIhttp://dx.doi.org/10.1099/jmm.0.45803-0
 
ISI Accession Number IDWOS:000225893300012
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLau, SKP
 
dc.contributor.authorWoo, PCY
 
dc.contributor.authorFung, AMY
 
dc.contributor.authorChan, KM
 
dc.contributor.authorWoo, GKS
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2010-09-06T07:47:54Z
 
dc.date.available2010-09-06T07:47:54Z
 
dc.date.issued2004
 
dc.description.abstractOwing to the difficulties in identifying anaerobic, non-sporulating, Gram-positive bacilli in clinical microbiology laboratories, the epidemiology and clinical spectrum of disease of many of these bacteria have been poorly understood. The application of 16S rRNA gene sequencing in characterizing bacteraemia due to anaerobic, non-sporulating Gram-positive bacilli during a 4-year period is described. The first case of Olsenella uli bacteraemia, in a patient with acute cholangitis, is also reported. Among 165 blood culture isolates of anaerobic, Gram-positive bacilli, 75 were identified as Propionibacterium acnes by phenotypic tests and 21 as members of other anaerobic, non-sporulating Gram-positive bacilli by 16S rRNA gene sequencing. Of these 96 isolates, 16 (17 %) were associated with cases of clinically significant bacteraemia, among which 10 (63 %) were caused by Eggerthella, four (25 %) by Lactobacillus and one (6 %) by each of Eubacterium tenue and O. uli. Five of the 10 Eggerthella isolates were Eggerthella lenta, whereas the other five belonged to two novel Eggerthella species, with Eggerthella hongkongensis being almost as prevalent as Eggerthella lenta. Underlying disease in the gastrointestinal tract, isolation of Eggerthella and Lactobacillus, and monomicrobial bacteraemia were associated with clinically significant bacteraemia, whereas isolation of P. acnes and polymicrobial bacteraemia were associated with pseudobacteraemia. Most patients with clinically significant bacteraemia had underlying diseases, with diseases in the gastrointestinal tract being most common. The overall mortality rate was 31 %. Immunocompromised patients with clinically significant bacteraemia due to anaerobic, non-sporulating, Gram-positive bacilli other than P. acnes should be treated with appropriate antibiotics. The unexpected frequency of isolation of Eggerthella from blood cultures and its association with clinically significant disease suggest that this genus is probably of high pathogenicity. Further studies to look for specific virulence factors are warranted.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Medical Microbiology, 2004, v. 53 n. 12, p. 1247-1253 [How to Cite?]
DOI: http://dx.doi.org/10.1099/jmm.0.45803-0
 
dc.identifier.doihttp://dx.doi.org/10.1099/jmm.0.45803-0
 
dc.identifier.epage1253
 
dc.identifier.hkuros100211
 
dc.identifier.isiWOS:000225893300012
 
dc.identifier.issn0022-2615
2013 Impact Factor: 2.266
2013 SCImago Journal Rankings: 1.025
 
dc.identifier.issue12
 
dc.identifier.openurl
 
dc.identifier.pmid15585505
 
dc.identifier.scopuseid_2-s2.0-11144241375
 
dc.identifier.spage1247
 
dc.identifier.urihttp://hdl.handle.net/10722/78877
 
dc.identifier.volume53
 
dc.languageeng
 
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Medical Microbiology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdult
 
dc.subject.meshAged
 
dc.subject.meshAged, 80 and over
 
dc.subject.meshBacteremia - diagnosis - microbiology
 
dc.subject.meshBacteria, Anaerobic - classification - isolation & purification
 
dc.subject.meshBacterial Typing Techniques - methods
 
dc.subject.meshFemale
 
dc.subject.meshGram-Positive Bacteria - classification - isolation & purification
 
dc.subject.meshGram-Positive Bacterial Infections - diagnosis - epidemiology - microbiology
 
dc.subject.meshHong Kong - epidemiology
 
dc.subject.meshHumans
 
dc.subject.meshMale
 
dc.subject.meshMiddle Aged
 
dc.subject.meshMolecular Epidemiology
 
dc.subject.meshRNA, Bacterial - genetics
 
dc.subject.meshRNA, Ribosomal, 16S - genetics
 
dc.subject.meshSpecies Specificity
 
dc.titleAnaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Lau, SKP</contributor.author>
<contributor.author>Woo, PCY</contributor.author>
<contributor.author>Fung, AMY</contributor.author>
<contributor.author>Chan, KM</contributor.author>
<contributor.author>Woo, GKS</contributor.author>
<contributor.author>Yuen, KY</contributor.author>
<date.accessioned>2010-09-06T07:47:54Z</date.accessioned>
<date.available>2010-09-06T07:47:54Z</date.available>
<date.issued>2004</date.issued>
<identifier.citation>Journal Of Medical Microbiology, 2004, v. 53 n. 12, p. 1247-1253</identifier.citation>
<identifier.issn>0022-2615</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/78877</identifier.uri>
<description.abstract>Owing to the difficulties in identifying anaerobic, non-sporulating, Gram-positive bacilli in clinical microbiology laboratories, the epidemiology and clinical spectrum of disease of many of these bacteria have been poorly understood. The application of 16S rRNA gene sequencing in characterizing bacteraemia due to anaerobic, non-sporulating Gram-positive bacilli during a 4-year period is described. The first case of Olsenella uli bacteraemia, in a patient with acute cholangitis, is also reported. Among 165 blood culture isolates of anaerobic, Gram-positive bacilli, 75 were identified as Propionibacterium acnes by phenotypic tests and 21 as members of other anaerobic, non-sporulating Gram-positive bacilli by 16S rRNA gene sequencing. Of these 96 isolates, 16 (17 %) were associated with cases of clinically significant bacteraemia, among which 10 (63 %) were caused by Eggerthella, four (25 %) by Lactobacillus and one (6 %) by each of Eubacterium tenue and O. uli. Five of the 10 Eggerthella isolates were Eggerthella lenta, whereas the other five belonged to two novel Eggerthella species, with Eggerthella hongkongensis being almost as prevalent as Eggerthella lenta. Underlying disease in the gastrointestinal tract, isolation of Eggerthella and Lactobacillus, and monomicrobial bacteraemia were associated with clinically significant bacteraemia, whereas isolation of P. acnes and polymicrobial bacteraemia were associated with pseudobacteraemia. Most patients with clinically significant bacteraemia had underlying diseases, with diseases in the gastrointestinal tract being most common. The overall mortality rate was 31 %. Immunocompromised patients with clinically significant bacteraemia due to anaerobic, non-sporulating, Gram-positive bacilli other than P. acnes should be treated with appropriate antibiotics. The unexpected frequency of isolation of Eggerthella from blood cultures and its association with clinically significant disease suggest that this genus is probably of high pathogenicity. Further studies to look for specific virulence factors are warranted.</description.abstract>
<language>eng</language>
<publisher>Society for General Microbiology. The Journal&apos;s web site is located at http://jmm.sgmjournals.org</publisher>
<relation.ispartof>Journal of Medical Microbiology</relation.ispartof>
<subject.mesh>Adult</subject.mesh>
<subject.mesh>Aged</subject.mesh>
<subject.mesh>Aged, 80 and over</subject.mesh>
<subject.mesh>Bacteremia - diagnosis - microbiology</subject.mesh>
<subject.mesh>Bacteria, Anaerobic - classification - isolation &amp; purification</subject.mesh>
<subject.mesh>Bacterial Typing Techniques - methods</subject.mesh>
<subject.mesh>Female</subject.mesh>
<subject.mesh>Gram-Positive Bacteria - classification - isolation &amp; purification</subject.mesh>
<subject.mesh>Gram-Positive Bacterial Infections - diagnosis - epidemiology - microbiology</subject.mesh>
<subject.mesh>Hong Kong - epidemiology</subject.mesh>
<subject.mesh>Humans</subject.mesh>
<subject.mesh>Male</subject.mesh>
<subject.mesh>Middle Aged</subject.mesh>
<subject.mesh>Molecular Epidemiology</subject.mesh>
<subject.mesh>RNA, Bacterial - genetics</subject.mesh>
<subject.mesh>RNA, Ribosomal, 16S - genetics</subject.mesh>
<subject.mesh>Species Specificity</subject.mesh>
<title>Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing</title>
<type>Article</type>
<identifier.openurl>http://library.hku.hk:4550/resserv?sid=HKU:IR&amp;issn=0022-2615&amp;volume=53&amp;issue=Pt 12&amp;spage=1247&amp;epage=53&amp;date=2004&amp;atitle=Anaerobic,+non-sporulating,+Gram-positive+bacilli+bacteraemia+characterized+by+16S+rRNA+gene+sequencing.</identifier.openurl>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1099/jmm.0.45803-0</identifier.doi>
<identifier.pmid>15585505</identifier.pmid>
<identifier.scopus>eid_2-s2.0-11144241375</identifier.scopus>
<identifier.hkuros>100211</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-11144241375&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>53</identifier.volume>
<identifier.issue>12</identifier.issue>
<identifier.spage>1247</identifier.spage>
<identifier.epage>1253</identifier.epage>
<identifier.isi>WOS:000225893300012</identifier.isi>
<publisher.place>United Kingdom</publisher.place>
</item>
Author Affiliations
  1. The University of Hong Kong