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Article: MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: A novel approach using lineage-specific genes

TitleMP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: A novel approach using lineage-specific genes
Authors
Issue Date2007
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2007, v. 45 n. 11, p. 3647-3654 How to Cite?
AbstractA highly reproducible and discriminative typing system is essential for better understanding of the epidemiology of Penicillium marneffei, the most important thermal dimorphic fungus causing respiratory, skin, and systemic mycosis in Southeast Asia. The sequences of 11 housekeeping genes were identical among 10 strains of P. marneffei, but those of MP1 and its 13 homologues, a novel superfamily of mannoproteins in the subdivision Pezizomycotina of Ascomycetes, mostly species of Penicillium and Aspergillus, showed significant variations. Therefore, a multilocus sequence typing (MLST) system for P. marneffei was constructed using MP1 (549 bp) and the four of its homologues (MPLP4 [337 bp], MPLP7 [347 bp], MPLP10 [546 bp], and MPLP13 [422 bp]) that showed the greatest variations. Among the 2,201 bp of the five loci, 183 polymorphic sites were observed in 44 strains of P. marneffei. The median number of alleles at each locus was five (range, 5 [MPLP4, MPLP7, and MPLP13] to 15 [MPLP10]). Four of the five genes had nonsynonymous substitution/synonymous substitution (dn/ds) ratios of >1. A total of 35 different sequence types (STs) were assigned to the 44 P. marneffei isolates, with 28 of the 35 STs identified only once. The discriminatory power was 0.9884. MP1 and its homologues were better than housekeeping genes for MLST in P. marneffei. Due to their more rapid evolutionary rates, lineage-specific genes may be better candidates than housekeeping genes for sequence-based typing, especially in microbes that evolve slowly or have evolved recently. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78857
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLau, CCYen_HK
dc.contributor.authorChong, KTKen_HK
dc.contributor.authorTse, Hen_HK
dc.contributor.authorTsang, DNCen_HK
dc.contributor.authorLee, RAen_HK
dc.contributor.authorTse, CWSen_HK
dc.contributor.authorQue, TLen_HK
dc.contributor.authorChung, LMWen_HK
dc.contributor.authorNgan, AHYen_HK
dc.contributor.authorHui, WTen_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:47:39Z-
dc.date.available2010-09-06T07:47:39Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2007, v. 45 n. 11, p. 3647-3654en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78857-
dc.description.abstractA highly reproducible and discriminative typing system is essential for better understanding of the epidemiology of Penicillium marneffei, the most important thermal dimorphic fungus causing respiratory, skin, and systemic mycosis in Southeast Asia. The sequences of 11 housekeeping genes were identical among 10 strains of P. marneffei, but those of MP1 and its 13 homologues, a novel superfamily of mannoproteins in the subdivision Pezizomycotina of Ascomycetes, mostly species of Penicillium and Aspergillus, showed significant variations. Therefore, a multilocus sequence typing (MLST) system for P. marneffei was constructed using MP1 (549 bp) and the four of its homologues (MPLP4 [337 bp], MPLP7 [347 bp], MPLP10 [546 bp], and MPLP13 [422 bp]) that showed the greatest variations. Among the 2,201 bp of the five loci, 183 polymorphic sites were observed in 44 strains of P. marneffei. The median number of alleles at each locus was five (range, 5 [MPLP4, MPLP7, and MPLP13] to 15 [MPLP10]). Four of the five genes had nonsynonymous substitution/synonymous substitution (dn/ds) ratios of >1. A total of 35 different sequence types (STs) were assigned to the 44 P. marneffei isolates, with 28 of the 35 STs identified only once. The discriminatory power was 0.9884. MP1 and its homologues were better than housekeeping genes for MLST in P. marneffei. Due to their more rapid evolutionary rates, lineage-specific genes may be better candidates than housekeeping genes for sequence-based typing, especially in microbes that evolve slowly or have evolved recently. Copyright © 2007, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshChild, Preschoolen_HK
dc.subject.meshElectrophoresis, Gel, Pulsed-Fielden_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshMycological Typing Techniques - methodsen_HK
dc.subject.meshPenicillium - classification - geneticsen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.titleMP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: A novel approach using lineage-specific genesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=45&spage=3647&epage=3654&date=2007&atitle=MP1+homologue-based+multilocus+sequence+system+for+typing+the+pathogenic+fungus+Penicillium+marneffei:+a+novel+approach+using+lineage-specific+genesen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTse, H:herman@graduate.hku.hken_HK
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTse, H=rp00519en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/JCM.00619-07en_HK
dc.identifier.pmid17881546-
dc.identifier.scopuseid_2-s2.0-36348960246en_HK
dc.identifier.hkuros149556en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36348960246&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue11en_HK
dc.identifier.spage3647en_HK
dc.identifier.epage3654en_HK
dc.identifier.isiWOS:000250932700026-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLau, CCY=8398162900en_HK
dc.identifier.scopusauthoridChong, KTK=7102553965en_HK
dc.identifier.scopusauthoridTse, H=7006070596en_HK
dc.identifier.scopusauthoridTsang, DNC=7005609132en_HK
dc.identifier.scopusauthoridLee, RA=7408203830en_HK
dc.identifier.scopusauthoridTse, CWS=7103295064en_HK
dc.identifier.scopusauthoridQue, TL=7003786628en_HK
dc.identifier.scopusauthoridChung, LMW=15847898900en_HK
dc.identifier.scopusauthoridNgan, AHY=14037517900en_HK
dc.identifier.scopusauthoridHui, WT=7103196480en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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