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Article: Well-characterized monoclonal antibodies against cell wall antigen of Aspergillus species improve immunoassay specificity and sensitivity

TitleWell-characterized monoclonal antibodies against cell wall antigen of Aspergillus species improve immunoassay specificity and sensitivity
Authors
Issue Date2008
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/
Citation
Clinical And Vaccine Immunology, 2008, v. 15 n. 2, p. 194-202 How to Cite?
AbstractThe diagnosis of invasive aspergillosis (IA) based on the detection of Aspergillus galactomannan (GM) is complicated by the presence of cross-reactive GM epitopes in patient specimens. We have developed a novel and specific Aspergillus antigen-capture enzyme-linked immunosorbent assay (ELISA) by the selection of two well-characterized monoclonal antibodies from 17 candidate antibodies. The epitopes recognized by the monoclonal antibodies were present on the cell walls of the hyphae and the conidia of Aspergillus species, which were circulating or excreted as immunodominant antigens during the acute phase of IA established in the animal models. The detection of experimental Aspergillus-mediated antigenemia was suitably sensitive, and the sensitivity was comparable to that of a commercial GM detection ELISA kit (the Platelia Aspergillus assay). Moreover, the specificity of this assay was 100% when it was used to test 382 serum specimens and 120 urine specimens from healthy individuals. Cross-reactivity with other common opportunistic fungi, such as Penicillium and Candida species, and with purified GM protein derived from Aspergillus was not evident. Therefore, the chemical nature of the epitopes captured in this assay is most likely not associated with the GM structure, indicating that this newly developed Aspergillus antigen-capture ELISA is a promising tool for the diagnosis of IA without the risk of the false-positive results that are problematic with current GM antigen assays. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78855
ISSN
2015 Impact Factor: 2.277
2015 SCImago Journal Rankings: 1.329
ISI Accession Number ID
Funding AgencyGrant Number
Research Program of the Natural Science Foundation06024435
Funding Information:

This work was supported by grant 06024435 of the Research Program of the Natural Science Foundation from the Guangdong Science and Technology Council, Guangdong Province, People's Republic of China.

References

 

DC FieldValueLanguage
dc.contributor.authorHao, Wen_HK
dc.contributor.authorPan, YXen_HK
dc.contributor.authorDing, YQen_HK
dc.contributor.authorXiao, Sen_HK
dc.contributor.authorYin, Ken_HK
dc.contributor.authorWang, YDen_HK
dc.contributor.authorQiu, LWen_HK
dc.contributor.authorZhang, QLen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorChe, XYen_HK
dc.date.accessioned2010-09-06T07:47:38Z-
dc.date.available2010-09-06T07:47:38Z-
dc.date.issued2008en_HK
dc.identifier.citationClinical And Vaccine Immunology, 2008, v. 15 n. 2, p. 194-202en_HK
dc.identifier.issn1556-6811en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78855-
dc.description.abstractThe diagnosis of invasive aspergillosis (IA) based on the detection of Aspergillus galactomannan (GM) is complicated by the presence of cross-reactive GM epitopes in patient specimens. We have developed a novel and specific Aspergillus antigen-capture enzyme-linked immunosorbent assay (ELISA) by the selection of two well-characterized monoclonal antibodies from 17 candidate antibodies. The epitopes recognized by the monoclonal antibodies were present on the cell walls of the hyphae and the conidia of Aspergillus species, which were circulating or excreted as immunodominant antigens during the acute phase of IA established in the animal models. The detection of experimental Aspergillus-mediated antigenemia was suitably sensitive, and the sensitivity was comparable to that of a commercial GM detection ELISA kit (the Platelia Aspergillus assay). Moreover, the specificity of this assay was 100% when it was used to test 382 serum specimens and 120 urine specimens from healthy individuals. Cross-reactivity with other common opportunistic fungi, such as Penicillium and Candida species, and with purified GM protein derived from Aspergillus was not evident. Therefore, the chemical nature of the epitopes captured in this assay is most likely not associated with the GM structure, indicating that this newly developed Aspergillus antigen-capture ELISA is a promising tool for the diagnosis of IA without the risk of the false-positive results that are problematic with current GM antigen assays. Copyright © 2008, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/ en_HK
dc.relation.ispartofClinical and Vaccine Immunologyen_HK
dc.rightsClinical and Vaccine Immunology. Copyright © American Society for Microbiology.en_HK
dc.titleWell-characterized monoclonal antibodies against cell wall antigen of Aspergillus species improve immunoassay specificity and sensitivityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1556-6811&volume=15&spage=194&epage=202&date=2008&atitle=Well-characterized+monoclonal+antibodies+against+cell+wall+antigen+of+Aspergillus+species+improve+immunoassay+specificity+and+sensitivityen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/CVI.00362-07en_HK
dc.identifier.scopuseid_2-s2.0-40449142278en_HK
dc.identifier.hkuros149574en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40449142278&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue2en_HK
dc.identifier.spage194en_HK
dc.identifier.epage202en_HK
dc.identifier.isiWOS:000258666600003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHao, W=7101686587en_HK
dc.identifier.scopusauthoridPan, YX=35765899800en_HK
dc.identifier.scopusauthoridDing, YQ=7404137178en_HK
dc.identifier.scopusauthoridXiao, S=55126464900en_HK
dc.identifier.scopusauthoridYin, K=55254969200en_HK
dc.identifier.scopusauthoridWang, YD=9638471800en_HK
dc.identifier.scopusauthoridQiu, LW=8719102700en_HK
dc.identifier.scopusauthoridZhang, QL=7406715600en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridChe, XY=7005743182en_HK

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