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Article: Functional analysis of naturally occurring mutations in the open reading frame of CCR5 in HIV-infected Chinese patients and healthy controls

TitleFunctional analysis of naturally occurring mutations in the open reading frame of CCR5 in HIV-infected Chinese patients and healthy controls
Authors
Issue Date2005
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.jaids.com
Citation
Journal Of Acquired Immune Deficiency Syndromes, 2005, v. 38 n. 5, p. 509-517 How to Cite?
AbstractWe studied polymorphism of the HIV coreceptor CC chemokine receptor (CCR) 5 in 1099 Chinese adults residing in Hong Kong, including 785 HIV-negative healthy donors and 314 HIV-positive patients. Ten mutants in the CCR5 open reading frame were identified, 7 of which were nonsynonymous. The frequencies of these alleles did not show a significant difference between HIV patients and healthy controls. G106R, Δ32, R223Q, 299(FS), and S3361 were cloned from prevalent mutant genes, and their effects on HIV infection were analyzed by a series of in vitro experiments to determine their transcription levels, expression levels, conformational changes, and HIV coreceptor function. R223Q is the most prevalent CCR5 mutant in ethnic Chinese, with a frequency of 0.046, which does not affect HIV infection in vitro, however. The S336I mutant also does not affect its transcription, expression, or HIV coreceptor function. Similar to 299(FS), the mutant G106R located in the third transmembrane domain results in diminished HIV coreceptor function in vitro through conformation changes in ECL2. Copyright © 2005 by Lippincott Williams & Wilkins.
Persistent Identifierhttp://hdl.handle.net/10722/78850
ISSN
2015 Impact Factor: 3.806
2015 SCImago Journal Rankings: 2.434
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhao, XYen_HK
dc.contributor.authorLee, SSen_HK
dc.contributor.authorWong, KHen_HK
dc.contributor.authorChan, KCWen_HK
dc.contributor.authorNg, Fen_HK
dc.contributor.authorChan, CCSen_HK
dc.contributor.authorHan, Den_HK
dc.contributor.authorYam, WCen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorNg, MHen_HK
dc.contributor.authorZheng, BJen_HK
dc.date.accessioned2010-09-06T07:47:34Z-
dc.date.available2010-09-06T07:47:34Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Acquired Immune Deficiency Syndromes, 2005, v. 38 n. 5, p. 509-517en_HK
dc.identifier.issn1525-4135en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78850-
dc.description.abstractWe studied polymorphism of the HIV coreceptor CC chemokine receptor (CCR) 5 in 1099 Chinese adults residing in Hong Kong, including 785 HIV-negative healthy donors and 314 HIV-positive patients. Ten mutants in the CCR5 open reading frame were identified, 7 of which were nonsynonymous. The frequencies of these alleles did not show a significant difference between HIV patients and healthy controls. G106R, Δ32, R223Q, 299(FS), and S3361 were cloned from prevalent mutant genes, and their effects on HIV infection were analyzed by a series of in vitro experiments to determine their transcription levels, expression levels, conformational changes, and HIV coreceptor function. R223Q is the most prevalent CCR5 mutant in ethnic Chinese, with a frequency of 0.046, which does not affect HIV infection in vitro, however. The S336I mutant also does not affect its transcription, expression, or HIV coreceptor function. Similar to 299(FS), the mutant G106R located in the third transmembrane domain results in diminished HIV coreceptor function in vitro through conformation changes in ECL2. Copyright © 2005 by Lippincott Williams & Wilkins.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.jaids.comen_HK
dc.relation.ispartofJournal of Acquired Immune Deficiency Syndromesen_HK
dc.rightsJournal of acquired immune deficiency syndromes. Copyright © Lippincott Williams & Wilkins.en_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshAmino Acid Substitutionen_HK
dc.subject.meshAsian Continental Ancestry Group - geneticsen_HK
dc.subject.meshChinaen_HK
dc.subject.meshGene Frequencyen_HK
dc.subject.meshHIV Infections - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshModels, Molecularen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshMutationen_HK
dc.subject.meshOpen Reading Framesen_HK
dc.subject.meshPolymorphism, Geneticen_HK
dc.subject.meshPolymorphism, Single Nucleotideen_HK
dc.subject.meshProtein Conformationen_HK
dc.subject.meshReceptors, CCR5 - geneticsen_HK
dc.subject.meshReference Valuesen_HK
dc.subject.meshTranscription, Geneticen_HK
dc.titleFunctional analysis of naturally occurring mutations in the open reading frame of CCR5 in HIV-infected Chinese patients and healthy controlsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0894-9255&volume=38&issue=5&spage=509&epage=17&date=2005&atitle=Functional+analysis+of+naturally+occurring+mutations+in+the+open+reading+frame+of+CCR5+in+HIV-infected+Chinese+patients+and+healthy+controls.en_HK
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1097/01.qai.0000151004.19128.4aen_HK
dc.identifier.pmid15793360-
dc.identifier.scopuseid_2-s2.0-20244365650en_HK
dc.identifier.hkuros100316en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-20244365650&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue5en_HK
dc.identifier.spage509en_HK
dc.identifier.epage517en_HK
dc.identifier.isiWOS:000228018200002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhao, XY=7407577215en_HK
dc.identifier.scopusauthoridLee, SS=7601394467en_HK
dc.identifier.scopusauthoridWong, KH=7404758411en_HK
dc.identifier.scopusauthoridChan, KCW=35097079800en_HK
dc.identifier.scopusauthoridNg, F=7103125273en_HK
dc.identifier.scopusauthoridChan, CCS=16021156900en_HK
dc.identifier.scopusauthoridHan, D=8326297800en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridNg, MH=7202076421en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK

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