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Article: Susceptibility patterns of clinical and fish isolates of Laribacter hongkongensis: Comparison of the Etest, disc diffusion and broth microdilution methods

TitleSusceptibility patterns of clinical and fish isolates of Laribacter hongkongensis: Comparison of the Etest, disc diffusion and broth microdilution methods
Authors
Issue Date2009
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
Citation
Journal Of Antimicrobial Chemotherapy, 2009, v. 63 n. 4, p. 704-708 How to Cite?
AbstractObjectives: To determine the antibiotic susceptibility patterns of 60 strains of Laribacter hongkongensis isolated from humans and fish to eight antibiotics and compare the results obtained from broth microdilution, Etest and disc diffusion susceptibility testing. Patients and methods: The susceptibilities of 60 isolates of L. hongkongensis from humans with gastroenteritis and fish to eight antibiotics were tested by three methods [broth microdilution (reference method), Etest and disc diffusion] and their results were compared. Results: All isolates were susceptible to imipenem and ciprofloxacin by all three methods, except for one strain which was resistant to ciprofloxacin by broth microdilution. All were susceptible to ampicillin/ sulbactam by Etest and disc diffusion, but eight were resistant by broth microdilution. By broth microdilution, 90%, 100%, 46.7%, 100% and 8.3% of isolates were resistant to ampicillin, ceftriaxone, cefuroxime, erythromycin and tetracycline, respectively. Although broth microdilution generally yielded higher MICs of β-lactams, MICs obtained with Etest were in good correlation with broth microdilution for all drugs except ampicillin/sulbactam, with >90% agreement within 2 log2 dilutions for imipenem, ciprofloxacin, erythromycin and tetracycline. Comparison of susceptibilities between broth microdilution and the other two methods showed the highest (>95%) percentage agreement for imipenem, ciprofloxacin and tetracycline. The highest discrepancies were observed with erythromycin (58.3% agreement), with an apparent increase in susceptibility by disc diffusion. A higher proportion of human isolates than fish isolates were tetracycline-resistant by all three tests (P = 0.022). Conclusions: Etest and disc diffusion appear to be reliable for evaluation of susceptibilities of L. hongkongensis to imipenem, ciprofloxacin and tetracycline. However, these methods may underestimate resistance to other β-lactams. © The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78835
ISSN
2015 Impact Factor: 4.919
2015 SCImago Journal Rankings: 2.157
ISI Accession Number ID
Funding AgencyGrant Number
University Research Grant CouncilHKU7531/05M
University Development Fund
HKU Special Research Achievement Award
The Croucher Senior Medical Research Fellowship
Outstanding Young Researcher Award
The University of Hong Kong
Research Fund for the Control of Infectious Diseases of the Health
Welfare and Food Bureau of the Hong Kong SAR Government
Funding Information:

This work is partly supported by the University Research Grant Council (grant HKU7531/05M); University Development Fund, HKU Special Research Achievement Award, The Croucher Senior Medical Research Fellowship 2006-2007, and Outstanding Young Researcher Award, The University of Hong Kong; and the Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau of the Hong Kong SAR Government.

References

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWong, GKMen_HK
dc.contributor.authorPoon, RWSen_HK
dc.contributor.authorLee, LCKen_HK
dc.contributor.authorLeung, KWen_HK
dc.contributor.authorTse, CWSen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorQue, TLen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:47:24Z-
dc.date.available2010-09-06T07:47:24Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2009, v. 63 n. 4, p. 704-708en_HK
dc.identifier.issn0305-7453en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78835-
dc.description.abstractObjectives: To determine the antibiotic susceptibility patterns of 60 strains of Laribacter hongkongensis isolated from humans and fish to eight antibiotics and compare the results obtained from broth microdilution, Etest and disc diffusion susceptibility testing. Patients and methods: The susceptibilities of 60 isolates of L. hongkongensis from humans with gastroenteritis and fish to eight antibiotics were tested by three methods [broth microdilution (reference method), Etest and disc diffusion] and their results were compared. Results: All isolates were susceptible to imipenem and ciprofloxacin by all three methods, except for one strain which was resistant to ciprofloxacin by broth microdilution. All were susceptible to ampicillin/ sulbactam by Etest and disc diffusion, but eight were resistant by broth microdilution. By broth microdilution, 90%, 100%, 46.7%, 100% and 8.3% of isolates were resistant to ampicillin, ceftriaxone, cefuroxime, erythromycin and tetracycline, respectively. Although broth microdilution generally yielded higher MICs of β-lactams, MICs obtained with Etest were in good correlation with broth microdilution for all drugs except ampicillin/sulbactam, with >90% agreement within 2 log2 dilutions for imipenem, ciprofloxacin, erythromycin and tetracycline. Comparison of susceptibilities between broth microdilution and the other two methods showed the highest (>95%) percentage agreement for imipenem, ciprofloxacin and tetracycline. The highest discrepancies were observed with erythromycin (58.3% agreement), with an apparent increase in susceptibility by disc diffusion. A higher proportion of human isolates than fish isolates were tetracycline-resistant by all three tests (P = 0.022). Conclusions: Etest and disc diffusion appear to be reliable for evaluation of susceptibilities of L. hongkongensis to imipenem, ciprofloxacin and tetracycline. However, these methods may underestimate resistance to other β-lactams. © The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/en_HK
dc.relation.ispartofJournal of Antimicrobial Chemotherapyen_HK
dc.rightsJournal Of Antimicrobial Chemotherapy. Copyright © Oxford University Press.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAnti-Bacterial Agents - pharmacologyen_HK
dc.subject.meshBacteriological Techniques - methodsen_HK
dc.subject.meshFish Diseases - microbiologyen_HK
dc.subject.meshFishesen_HK
dc.subject.meshGastroenteritis - microbiologyen_HK
dc.subject.meshGram-Positive Bacterial Infections - microbiology - veterinaryen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMicrobial Sensitivity Tests - methodsen_HK
dc.subject.meshNeisseriaceae - isolation & purificationen_HK
dc.titleSusceptibility patterns of clinical and fish isolates of Laribacter hongkongensis: Comparison of the Etest, disc diffusion and broth microdilution methodsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0305-7453&volume=63&spage=704&epage=708&date=2009&atitle=Susceptibility+Patterns+Of+Clinical+And+Fish+Isolates+Of+Laribacter+Hongkongensis:+Comparison+Of+The+Etest,+Disc+Diffusion+And+Broth+Microdilution+Methodsen_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/jac/dkp010en_HK
dc.identifier.pmid19202149-
dc.identifier.scopuseid_2-s2.0-62549092696en_HK
dc.identifier.hkuros161676en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-62549092696&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume63en_HK
dc.identifier.issue4en_HK
dc.identifier.spage704en_HK
dc.identifier.epage708en_HK
dc.identifier.isiWOS:000264191100012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWong, GKM=9333006300en_HK
dc.identifier.scopusauthoridPoon, RWS=9334879200en_HK
dc.identifier.scopusauthoridLee, LCK=25650155400en_HK
dc.identifier.scopusauthoridLeung, KW=7401860831en_HK
dc.identifier.scopusauthoridTse, CWS=7103295064en_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridQue, TL=7003786628en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.citeulike4245243-

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