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Article: Diagnosis of pelvic actinomycosis by 16S ribosomal RNA gene sequencing and its clinical significance

TitleDiagnosis of pelvic actinomycosis by 16S ribosomal RNA gene sequencing and its clinical significance
Authors
Issue Date2002
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobio
Citation
Diagnostic Microbiology And Infectious Disease, 2002, v. 43 n. 2, p. 113-118 How to Cite?
AbstractTraditional ways of identification of anaerobic Gram-positive non-sporulating bacilli by isolation of the organism and studying it phenotypically by elucidation of its morphologic and biochemical characteristics and metabolic end products are associated with a need for special equipment and expertise, and strains that are "unidentified" because of ambiguous biochemical profiles. In this study, an anaerobic Gram-positive non-sporulating bacterium was isolated from the intrauterine contraceptive device of a 36-year old woman with pyosalpinx. The Vitek system (ANI) showed that it was 99% Propionibacterium granulosum; whereas the API system (20A) showed that it was 78% Actinomyces meyeri/odontolyticus. The 16S ribosomal RNA gene of the strain was amplified and sequenced. There was 0 base difference between the isolate and A. odontolyticus (GenBank Accession no. AJ234047), indicating the isolate most closely resembled a strain of A. odontolyticus. Identification of the organism in this study was important because the duration of antibiotic therapy would be entirely different. In the present case, identification of the bacterium as A. odontolyticus inferred that the patient suffered from an intermediate form of pelvic actinomycosis. A prolonged course of antibiotics would be more desirable, as the relapse rate of actinomycosis after a short course of antibiotics is high. © 2002 Elsevier Science Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78804
ISSN
2015 Impact Factor: 2.45
2015 SCImago Journal Rankings: 1.142
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorFung, AMYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorHon, Een_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:47:00Z-
dc.date.available2010-09-06T07:47:00Z-
dc.date.issued2002en_HK
dc.identifier.citationDiagnostic Microbiology And Infectious Disease, 2002, v. 43 n. 2, p. 113-118en_HK
dc.identifier.issn0732-8893en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78804-
dc.description.abstractTraditional ways of identification of anaerobic Gram-positive non-sporulating bacilli by isolation of the organism and studying it phenotypically by elucidation of its morphologic and biochemical characteristics and metabolic end products are associated with a need for special equipment and expertise, and strains that are "unidentified" because of ambiguous biochemical profiles. In this study, an anaerobic Gram-positive non-sporulating bacterium was isolated from the intrauterine contraceptive device of a 36-year old woman with pyosalpinx. The Vitek system (ANI) showed that it was 99% Propionibacterium granulosum; whereas the API system (20A) showed that it was 78% Actinomyces meyeri/odontolyticus. The 16S ribosomal RNA gene of the strain was amplified and sequenced. There was 0 base difference between the isolate and A. odontolyticus (GenBank Accession no. AJ234047), indicating the isolate most closely resembled a strain of A. odontolyticus. Identification of the organism in this study was important because the duration of antibiotic therapy would be entirely different. In the present case, identification of the bacterium as A. odontolyticus inferred that the patient suffered from an intermediate form of pelvic actinomycosis. A prolonged course of antibiotics would be more desirable, as the relapse rate of actinomycosis after a short course of antibiotics is high. © 2002 Elsevier Science Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobioen_HK
dc.relation.ispartofDiagnostic Microbiology and Infectious Diseaseen_HK
dc.rightsDiagnostic Microbiology and Infectious Disease. Copyright © Elsevier Inc.en_HK
dc.subject.meshActinomyces - classification - genetics - isolation & purificationen_HK
dc.subject.meshActinomycosis - diagnosis - microbiologyen_HK
dc.subject.meshAdulten_HK
dc.subject.meshEquipment Contaminationen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIntrauterine Devices - microbiologyen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshRNA, Ribosomal, 16S - geneticsen_HK
dc.subject.meshSalpingitis - microbiologyen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.titleDiagnosis of pelvic actinomycosis by 16S ribosomal RNA gene sequencing and its clinical significanceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0732-8893&volume=43&spage=113&epage=118&date=2002&atitle=Diagnosis+of+pelvic+actinomycosis+by+16S+ribosomal+RNA+gene+sequencing+and+its+clinical+significanceen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0732-8893(02)00375-9en_HK
dc.identifier.pmid12088617-
dc.identifier.scopuseid_2-s2.0-0036092611en_HK
dc.identifier.hkuros74440en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036092611&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue2en_HK
dc.identifier.spage113en_HK
dc.identifier.epage118en_HK
dc.identifier.isiWOS:000177022500004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridFung, AMY=7101926801en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridHon, E=7005164611en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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