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Article: Paracrine effects of direct intramyocardial implantation of bone marrow derived cells to enhance neovascularization in chronic ischaemic myocardium

TitleParacrine effects of direct intramyocardial implantation of bone marrow derived cells to enhance neovascularization in chronic ischaemic myocardium
Authors
KeywordsBone marrow
Ischaemic myocardium
Neovascularization
Issue Date2007
PublisherOxford University Press. The Journal's web site is located at http://eurjhf.oxfordjournals.org
Citation
European Journal of Heart Failure, 2007, v. 9 n. 8, p. 747-753 How to Cite?
AbstractObjective: To determine the optimal bone marrow (BM) cell types, and their potential mechanisms of action for neovascularization in chronic ischaemic myocardium. Methods and results: The functional effects, angiogenic potential and cytokine expression of direct intramyocardial implantation of autologous BM CD31-positive endothelial progenitor cells (EPC, n = 9), BM mononuclear cells (MNCs, n = 9), and saline (n = 9) were compared in a swine model of chronic ischaemic myocardium. Autologous BM cells were harvested and catheter-based electromechanical mapping-guided direct intramyocardial injection was performed to target ischaemic myocardium. After 12 weeks, injection of BM-MNC resulted in significant improvements in left ventricular dP / dt (+ 21 ± 8%, P = 0.032), left ventricular pressure (+ 17 ± 4%, P = 0.048) and regional microsphere myocardial perfusion over ischaemic endocardium (+ 74 ± 28%, P < 0.05) and epicardium (+73 ± 29%, P < 0.05). No significant effects were observed following injection of BM-EPC or saline. Capillary density (1132 ± 69 versus 903 ± 44 per mm 2, P = 0.047) and expression of mRNA of vascular endothelial growth factor (VEGF, 32.3 ± 5.6 versus 13.1 ± 3.7, P < 0.05,) and angiopoietin-2 (23.9 ± 3.6 versus 13.7 ± 3.1, P < 0.05) in ischaemic myocardium was significantly greater in the BM-MNC group than the saline group. The capillary density in ischaemic myocardium demonstrated a significant positive correlation with VEGF expression (r = 0.61, P < 0.001). Conclusion: Catheter-based direct intramyocardial injection of BM-MNC enhanced angiogenesis more effectively than BM-EPC or saline, possibly via a paracrine effect, with increased expression of VEGF that subsequently improved cardiac performance of ischaemic myocardium. © 2007 European Society of Cardiology.
Persistent Identifierhttp://hdl.handle.net/10722/78698
ISSN
2015 Impact Factor: 5.135
2015 SCImago Journal Rankings: 3.638
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTse, HFen_HK
dc.contributor.authorSiu, CWen_HK
dc.contributor.authorZhu, SGen_HK
dc.contributor.authorLiao, SYen_HK
dc.contributor.authorZhang, QYen_HK
dc.contributor.authorLai, WHen_HK
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorNicholls, Jen_HK
dc.contributor.authorLau, CPen_HK
dc.date.accessioned2010-09-06T07:45:44Z-
dc.date.available2010-09-06T07:45:44Z-
dc.date.issued2007en_HK
dc.identifier.citationEuropean Journal of Heart Failure, 2007, v. 9 n. 8, p. 747-753en_HK
dc.identifier.issn1388-9842en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78698-
dc.description.abstractObjective: To determine the optimal bone marrow (BM) cell types, and their potential mechanisms of action for neovascularization in chronic ischaemic myocardium. Methods and results: The functional effects, angiogenic potential and cytokine expression of direct intramyocardial implantation of autologous BM CD31-positive endothelial progenitor cells (EPC, n = 9), BM mononuclear cells (MNCs, n = 9), and saline (n = 9) were compared in a swine model of chronic ischaemic myocardium. Autologous BM cells were harvested and catheter-based electromechanical mapping-guided direct intramyocardial injection was performed to target ischaemic myocardium. After 12 weeks, injection of BM-MNC resulted in significant improvements in left ventricular dP / dt (+ 21 ± 8%, P = 0.032), left ventricular pressure (+ 17 ± 4%, P = 0.048) and regional microsphere myocardial perfusion over ischaemic endocardium (+ 74 ± 28%, P < 0.05) and epicardium (+73 ± 29%, P < 0.05). No significant effects were observed following injection of BM-EPC or saline. Capillary density (1132 ± 69 versus 903 ± 44 per mm 2, P = 0.047) and expression of mRNA of vascular endothelial growth factor (VEGF, 32.3 ± 5.6 versus 13.1 ± 3.7, P < 0.05,) and angiopoietin-2 (23.9 ± 3.6 versus 13.7 ± 3.1, P < 0.05) in ischaemic myocardium was significantly greater in the BM-MNC group than the saline group. The capillary density in ischaemic myocardium demonstrated a significant positive correlation with VEGF expression (r = 0.61, P < 0.001). Conclusion: Catheter-based direct intramyocardial injection of BM-MNC enhanced angiogenesis more effectively than BM-EPC or saline, possibly via a paracrine effect, with increased expression of VEGF that subsequently improved cardiac performance of ischaemic myocardium. © 2007 European Society of Cardiology.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://eurjhf.oxfordjournals.orgen_HK
dc.relation.ispartofEuropean Journal of Heart Failureen_HK
dc.rightsEuropean journal of heart failure. Copyright © Elsevier BV.en_HK
dc.rightsEuropean Journal of Heart Failure. Copyright © Elsevier BV.-
dc.subjectBone marrowen_HK
dc.subjectIschaemic myocardiumen_HK
dc.subjectNeovascularizationen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBone Marrow Transplantation - methods - physiologyen_HK
dc.subject.meshCell Counten_HK
dc.subject.meshCell Differentiationen_HK
dc.subject.meshChronic Diseaseen_HK
dc.subject.meshElectrophysiologic Techniques, Cardiacen_HK
dc.subject.meshFlow Cytometryen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshLeukocytes, Mononuclear - physiologyen_HK
dc.subject.meshMicrospheresen_HK
dc.subject.meshMyocardial Ischemia - physiopathologyen_HK
dc.subject.meshMyocardial Revascularization - methodsen_HK
dc.subject.meshMyocardium - metabolismen_HK
dc.subject.meshNeovascularization, Physiologicen_HK
dc.subject.meshParacrine Communicationen_HK
dc.subject.meshStem Cells - physiologyen_HK
dc.subject.meshSwineen_HK
dc.subject.meshSwine, Miniatureen_HK
dc.subject.meshVascular Endothelial Growth Factor A - metabolismen_HK
dc.subject.meshVentricular Function, Leften_HK
dc.titleParacrine effects of direct intramyocardial implantation of bone marrow derived cells to enhance neovascularization in chronic ischaemic myocardiumen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1388-9842&volume=9&issue=8&spage=747&epage=753&date=2007&atitle=Paracrine+effects+of+direct+intramyocardial+implantation+of+bone+marrow+derived+cells+to+enhance+neovascularization+in+chronic+ischaemic+myocardiumen_HK
dc.identifier.emailTse, HF: hftse@hkucc.hku.hken_HK
dc.identifier.emailSiu, CW: cwdsiu@hkucc.hku.hken_HK
dc.identifier.emailKwong, YL: ylkwong@hku.hken_HK
dc.identifier.emailNicholls, J: nicholls@pathology.hku.hken_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.identifier.authoritySiu, CW=rp00534en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.authorityNicholls, J=rp00364en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.ejheart.2007.03.008en_HK
dc.identifier.pmid17481945-
dc.identifier.scopuseid_2-s2.0-34547670282en_HK
dc.identifier.hkuros126511en_HK
dc.identifier.hkuros146340-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34547670282&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue8en_HK
dc.identifier.spage747en_HK
dc.identifier.epage753en_HK
dc.identifier.isiWOS:000249363100001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.scopusauthoridSiu, CW=7006550690en_HK
dc.identifier.scopusauthoridZhu, SG=55237337600en_HK
dc.identifier.scopusauthoridSongyan, L=18435011600en_HK
dc.identifier.scopusauthoridZhang, QY=35331268500en_HK
dc.identifier.scopusauthoridLai, WH=18434390500en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridNicholls, J=7201463077en_HK
dc.identifier.scopusauthoridLau, CP=7401968501en_HK
dc.customcontrol.immutablesml 130621-

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