File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Catabolism of newly synthesized decorin in vitro by human peritoneal mesothelial cells

TitleCatabolism of newly synthesized decorin in vitro by human peritoneal mesothelial cells
Authors
Issue Date2004
PublisherMultimed, Inc. The Journal's web site is located at http://pdiconnect.com
Citation
Peritoneal Dialysis International, 2004, v. 24 n. 2, p. 147-155 How to Cite?
Abstract◆ Objective: Previous studies have shown that decorin and biglycan account for over 70% of the proteoglycans (PGs) synthesized by human peritoneal mesothelial cells (HPMCs). Since these PGs are involved in the control of cell growth, cell differentiation, and matrix assembly, we investigated their turnover in cultured HPMCs. ◆ Methods: Confluent HPMCs were metabolically labeled with [ 35S]-sulfate and the labeled products isolated from the cell medium and the cell layer characterized by sensitivity to bacterial eliminases. Experiments were undertaken with exogenous labeled decorin, and its metabolic state was studied. ◆ Results: In a 24-hour labeling period, 75% of the newly synthesized chondroitin sulfate/dermatan sulfate (CS/DS) PGs appeared in the culture medium, the majority of which (90%) was decorin. In the cell layer, protein-free glycosaminoglycan (GAG) chains accounted for 21% of the total CS/DS at 24 hours and exhibited constant specific activity at 12-16 hours. The latter material was turned over with a half-life of approximately 2.5 hours. Exogenous decorin underwent receptor-mediated endocytosis and subsequent intracellular degradation. Uptake but not degradation could be inhibited by heparin. ◆ Conclusions: HPMCs are distinguished by a rapid turnover of decorin. A characteristic metabolic feature is the existence of a large intracellular pool of protein-free DS-GAGs. Understanding the control of decorin turnover in HPMCs might lead to delineation of its potential role in both the physiology and pathophysiology of the membrane in PD patients. Copyright © 2004 International Society for Peritoneal Dialysis.
Persistent Identifierhttp://hdl.handle.net/10722/78647
ISSN
2015 Impact Factor: 1.298
2015 SCImago Journal Rankings: 0.683
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYung, Sen_HK
dc.contributor.authorHausser, Hen_HK
dc.contributor.authorThomas, Gen_HK
dc.contributor.authorSchaefer, Len_HK
dc.contributor.authorKresse, Hen_HK
dc.contributor.authorDavies, Men_HK
dc.date.accessioned2010-09-06T07:45:12Z-
dc.date.available2010-09-06T07:45:12Z-
dc.date.issued2004en_HK
dc.identifier.citationPeritoneal Dialysis International, 2004, v. 24 n. 2, p. 147-155en_HK
dc.identifier.issn0896-8608en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78647-
dc.description.abstract◆ Objective: Previous studies have shown that decorin and biglycan account for over 70% of the proteoglycans (PGs) synthesized by human peritoneal mesothelial cells (HPMCs). Since these PGs are involved in the control of cell growth, cell differentiation, and matrix assembly, we investigated their turnover in cultured HPMCs. ◆ Methods: Confluent HPMCs were metabolically labeled with [ 35S]-sulfate and the labeled products isolated from the cell medium and the cell layer characterized by sensitivity to bacterial eliminases. Experiments were undertaken with exogenous labeled decorin, and its metabolic state was studied. ◆ Results: In a 24-hour labeling period, 75% of the newly synthesized chondroitin sulfate/dermatan sulfate (CS/DS) PGs appeared in the culture medium, the majority of which (90%) was decorin. In the cell layer, protein-free glycosaminoglycan (GAG) chains accounted for 21% of the total CS/DS at 24 hours and exhibited constant specific activity at 12-16 hours. The latter material was turned over with a half-life of approximately 2.5 hours. Exogenous decorin underwent receptor-mediated endocytosis and subsequent intracellular degradation. Uptake but not degradation could be inhibited by heparin. ◆ Conclusions: HPMCs are distinguished by a rapid turnover of decorin. A characteristic metabolic feature is the existence of a large intracellular pool of protein-free DS-GAGs. Understanding the control of decorin turnover in HPMCs might lead to delineation of its potential role in both the physiology and pathophysiology of the membrane in PD patients. Copyright © 2004 International Society for Peritoneal Dialysis.en_HK
dc.languageengen_HK
dc.publisherMultimed, Inc. The Journal's web site is located at http://pdiconnect.comen_HK
dc.relation.ispartofPeritoneal Dialysis Internationalen_HK
dc.subject.meshCell Culture Techniquesen_HK
dc.subject.meshChondroitin Sulfates - biosynthesisen_HK
dc.subject.meshDecorinen_HK
dc.subject.meshDermatan Sulfate - biosynthesisen_HK
dc.subject.meshEndocytosisen_HK
dc.subject.meshEpithelial Cells - metabolismen_HK
dc.subject.meshExtracellular Matrix Proteinsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshOmentum - cytology - metabolismen_HK
dc.subject.meshProteoglycans - metabolismen_HK
dc.titleCatabolism of newly synthesized decorin in vitro by human peritoneal mesothelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0896-8608&volume=24&spage=147&epage=&date=2004&atitle=Catabolism+of+newly+synthesized+decorin+in+vitro+by+human+peritoneal+mesothelial+cellsen_HK
dc.identifier.emailYung, S:ssyyung@hku.hken_HK
dc.identifier.authorityYung, S=rp00455en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid15119635-
dc.identifier.scopuseid_2-s2.0-1942454220en_HK
dc.identifier.hkuros88137en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1942454220&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue2en_HK
dc.identifier.spage147en_HK
dc.identifier.epage155en_HK
dc.identifier.isiWOS:000220943300005-
dc.publisher.placeCanadaen_HK
dc.identifier.scopusauthoridYung, S=22636568800en_HK
dc.identifier.scopusauthoridHausser, H=7003266332en_HK
dc.identifier.scopusauthoridThomas, G=35464458200en_HK
dc.identifier.scopusauthoridSchaefer, L=7102364289en_HK
dc.identifier.scopusauthoridKresse, H=7102125144en_HK
dc.identifier.scopusauthoridDavies, M=7404207291en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats