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Article: Protective role of heme oxygenase-1 on trinitrobenzene sulfonic acid-induced colitis in rats

TitleProtective role of heme oxygenase-1 on trinitrobenzene sulfonic acid-induced colitis in rats
Authors
KeywordsInducible nitric oxide synthase
Reactive oxygen species
Issue Date2001
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/
Citation
American Journal Of Physiology - Gastrointestinal And Liver Physiology, 2001, v. 281 n. 2 44-2, p. G586-G594 How to Cite?
AbstractPreliminary studies showed that the inducible form of heme oxygenase (HO-1) was induced and played a protective role in the process of inflammation. The present study investigated the possible role of HO-1 in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats. We measured HO-1 activity in TNBS-induced colitis in rats and analyzed the severity of colitis along with altered HO activity by assessing lesion area and myeloperoxidase activity. HO-1 mRNA and protein expressions were determined at different time points after TNBS induction. Free radical production and inducible nitric oxide synthase (iNOS), which participate in oxidative injury, were also assayed. HO activity and HO-1 gene expression increased markedly after TNBS induction. Administration with tin mesoporphyrin (SnMP), a HO inhibitor, potentiated the colonic damage along with a reduction in HO-1 activity. Furthermore, the reduction of HO-1 expression by SnMP also enhanced reactive oxygen species and iNOS expression, both of which were dramatically increased after the TNBS enema. L-Arginine preteatment further aggravated the injurious action of SnMP. Our results indicate that HO-1 plays a protective role in the colonic damage induced by the TNBS enema, and the preventive effects probably result from decreased free radical production and inhibition of iNOS expression in colonic tissues.
Persistent Identifierhttp://hdl.handle.net/10722/78645
ISSN
2015 Impact Factor: 3.297
2015 SCImago Journal Rankings: 1.936
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, WPen_HK
dc.contributor.authorGuo, Xen_HK
dc.contributor.authorKoo, MWLen_HK
dc.contributor.authorWong, BCYen_HK
dc.contributor.authorLam, SKen_HK
dc.contributor.authorYe, YNen_HK
dc.contributor.authorCho, CHen_HK
dc.date.accessioned2010-09-06T07:45:10Z-
dc.date.available2010-09-06T07:45:10Z-
dc.date.issued2001en_HK
dc.identifier.citationAmerican Journal Of Physiology - Gastrointestinal And Liver Physiology, 2001, v. 281 n. 2 44-2, p. G586-G594en_HK
dc.identifier.issn0193-1857en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78645-
dc.description.abstractPreliminary studies showed that the inducible form of heme oxygenase (HO-1) was induced and played a protective role in the process of inflammation. The present study investigated the possible role of HO-1 in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats. We measured HO-1 activity in TNBS-induced colitis in rats and analyzed the severity of colitis along with altered HO activity by assessing lesion area and myeloperoxidase activity. HO-1 mRNA and protein expressions were determined at different time points after TNBS induction. Free radical production and inducible nitric oxide synthase (iNOS), which participate in oxidative injury, were also assayed. HO activity and HO-1 gene expression increased markedly after TNBS induction. Administration with tin mesoporphyrin (SnMP), a HO inhibitor, potentiated the colonic damage along with a reduction in HO-1 activity. Furthermore, the reduction of HO-1 expression by SnMP also enhanced reactive oxygen species and iNOS expression, both of which were dramatically increased after the TNBS enema. L-Arginine preteatment further aggravated the injurious action of SnMP. Our results indicate that HO-1 plays a protective role in the colonic damage induced by the TNBS enema, and the preventive effects probably result from decreased free radical production and inhibition of iNOS expression in colonic tissues.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Gastrointestinal and Liver Physiologyen_HK
dc.subjectInducible nitric oxide synthaseen_HK
dc.subjectReactive oxygen speciesen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshColitis, Ulcerative - chemically induced - enzymology - pathologyen_HK
dc.subject.meshEnzyme Inhibitors - pharmacologyen_HK
dc.subject.meshHeme Oxygenase (Decyclizing) - biosynthesis - genetics - immunology - physiologyen_HK
dc.subject.meshHeme Oxygenase-1en_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshLuminescent Measurementsen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMetalloporphyrins - pharmacologyen_HK
dc.subject.meshNitric Oxide Synthase - antagonists & inhibitorsen_HK
dc.subject.meshNitric Oxide Synthase Type IIen_HK
dc.subject.meshPeroxidase - metabolismen_HK
dc.subject.meshRNA, Messenger - biosynthesisen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReactive Oxygen Species - metabolismen_HK
dc.subject.meshTrinitrobenzenesulfonic Aciden_HK
dc.titleProtective role of heme oxygenase-1 on trinitrobenzene sulfonic acid-induced colitis in ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0193-1857&volume=281&spage=G586&epage=G594&date=2001&atitle=Protective+role+of+heme+oxygenase-1+on+trinitrobenzene+sulfonic+acid-induced+colitis+in+ratsen_HK
dc.identifier.emailKoo, MWL: wlkoo@hku.hken_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.authorityKoo, MWL=rp00233en_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11447040-
dc.identifier.scopuseid_2-s2.0-0034880733en_HK
dc.identifier.hkuros73369en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034880733&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume281en_HK
dc.identifier.issue2 44-2en_HK
dc.identifier.spageG586en_HK
dc.identifier.epageG594en_HK
dc.identifier.isiWOS:000169841200033-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, WP=7501765704en_HK
dc.identifier.scopusauthoridGuo, X=55202826300en_HK
dc.identifier.scopusauthoridKoo, MWL=7004550899en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK
dc.identifier.scopusauthoridLam, SK=7402279473en_HK
dc.identifier.scopusauthoridYe, YN=7401627402en_HK
dc.identifier.scopusauthoridCho, CH=14067000400en_HK

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