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Article: Functional mesenchymal stem cells derived from human induced pluripotent stem cells attenuate limb ischemia in mice

TitleFunctional mesenchymal stem cells derived from human induced pluripotent stem cells attenuate limb ischemia in mice
Authors
KeywordsInduced pluripotent stem cells
Ischemia
Mesenchymal stem cells
Peripheral vascular disease
Repair
Transplantation
Issue Date2010
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.org
Citation
Circulation, 2010, v. 121 n. 9, p. 1113-1123 How to Cite?
AbstractBackground: Aging and aging-related disorders impair the survival and differentiation potential of bone marrow mesenchymal stem cells (MSCs) and limit their therapeutic efficacy. Induced pluripotent stem cells (iPSCs) may provide an alternative source of functional MSCs for tissue repair. This study aimed to generate and characterize human iPSC-derived MSCs and to investigate their biological function for the treatment of limb ischemia. Methods and results: Human iPSCs were induced to MSC differentiation with a clinically compliant protocol. Three monoclonal, karyotypically stable, and functional MSC-like cultures were successfully isolated using a combination of CD24 and CD105 sorting. They did not express pluripotent-associated markers but displayed MSC surface antigens and differentiated into adipocytes, osteocytes, and chondrocytes. Transplanting iPSC-MSCs into mice significantly attenuated severe hind-limb ischemia and promoted vascular and muscle regeneration. The benefits of iPSC-MSCs on limb ischemia were superior to those of adult bone marrow MSCs. The greater potential of iPSC-MSCs may be attributable to their superior survival and engraftment after transplantation to induce vascular and muscle regeneration via direct de novo differentiation and paracrine mechanisms. Clusions: Functional MSCs can be clonally generated, beginning at a single-cell level, from human iPSCs. Patient-specific iPSC-MSCs can be prepared as an "off-the-shelf" format for the treatment of tissue ischemia. Copyright © 2010 American Heart Association. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78598
ISSN
2015 Impact Factor: 17.047
2015 SCImago Journal Rankings: 7.853
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong10208618
Hong Kong Research Grant CouncilHKU 763306M
HKU 7747/08M
Funding Information:

This research was supported by Seed Funding for Basic Research ( 10208618 to Dr Lian) and HKU Strategic Research Theme on Healthy Ageing (Drs Lian and Tse) from the University of Hong Kong and in part by a Hong Kong Research Grant Council General Research Fund (HKU 763306M and HKU 7747/08M to Drs Siu and Tse).

References

 

DC FieldValueLanguage
dc.contributor.authorLian, Qen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorZhang, Jen_HK
dc.contributor.authorZhang, HKen_HK
dc.contributor.authorWu, Xen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorLam, FFYen_HK
dc.contributor.authorKang, Sen_HK
dc.contributor.authorXia, JCen_HK
dc.contributor.authorLai, WHen_HK
dc.contributor.authorAu, KWen_HK
dc.contributor.authorChow, YYen_HK
dc.contributor.authorSiu, CWen_HK
dc.contributor.authorLee, CNen_HK
dc.contributor.authorTse, HFen_HK
dc.date.accessioned2010-09-06T07:44:39Z-
dc.date.available2010-09-06T07:44:39Z-
dc.date.issued2010en_HK
dc.identifier.citationCirculation, 2010, v. 121 n. 9, p. 1113-1123en_HK
dc.identifier.issn0009-7322en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78598-
dc.description.abstractBackground: Aging and aging-related disorders impair the survival and differentiation potential of bone marrow mesenchymal stem cells (MSCs) and limit their therapeutic efficacy. Induced pluripotent stem cells (iPSCs) may provide an alternative source of functional MSCs for tissue repair. This study aimed to generate and characterize human iPSC-derived MSCs and to investigate their biological function for the treatment of limb ischemia. Methods and results: Human iPSCs were induced to MSC differentiation with a clinically compliant protocol. Three monoclonal, karyotypically stable, and functional MSC-like cultures were successfully isolated using a combination of CD24 and CD105 sorting. They did not express pluripotent-associated markers but displayed MSC surface antigens and differentiated into adipocytes, osteocytes, and chondrocytes. Transplanting iPSC-MSCs into mice significantly attenuated severe hind-limb ischemia and promoted vascular and muscle regeneration. The benefits of iPSC-MSCs on limb ischemia were superior to those of adult bone marrow MSCs. The greater potential of iPSC-MSCs may be attributable to their superior survival and engraftment after transplantation to induce vascular and muscle regeneration via direct de novo differentiation and paracrine mechanisms. Clusions: Functional MSCs can be clonally generated, beginning at a single-cell level, from human iPSCs. Patient-specific iPSC-MSCs can be prepared as an "off-the-shelf" format for the treatment of tissue ischemia. Copyright © 2010 American Heart Association. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.orgen_HK
dc.relation.ispartofCirculationen_HK
dc.subjectInduced pluripotent stem cellsen_HK
dc.subjectIschemiaen_HK
dc.subjectMesenchymal stem cellsen_HK
dc.subjectPeripheral vascular diseaseen_HK
dc.subjectRepairen_HK
dc.subjectTransplantationen_HK
dc.subject.meshAdipocytes - cytology-
dc.subject.meshHindlimb - blood supply-
dc.subject.meshIschemia - surgery-
dc.subject.meshMesenchymal Stem Cells - physiology-
dc.subject.meshPluripotent Stem Cells - cytology - transplantation-
dc.titleFunctional mesenchymal stem cells derived from human induced pluripotent stem cells attenuate limb ischemia in miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0009-7322&volume=121&issue=9&spage=1113&epage=1123&date=2010&atitle=Functional+mesenchymal+stem+cells+derived+from+human+induced+pluripotent+stem+cells+attenuate+limb+ischemic+in+miceen_HK
dc.identifier.emailLian, Q:qzlian@hkucc.hku.hken_HK
dc.identifier.emailSiu, CW:cwdsiu@hkucc.hku.hken_HK
dc.identifier.emailTse, HF:hftse@hkucc.hku.hken_HK
dc.identifier.authorityLian, Q=rp00267en_HK
dc.identifier.authoritySiu, CW=rp00534en_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1161/CIRCULATIONAHA.109.898312en_HK
dc.identifier.pmid20176987en_HK
dc.identifier.scopuseid_2-s2.0-77949350004en_HK
dc.identifier.hkuros169694en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77949350004&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume121en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1113en_HK
dc.identifier.epage1123en_HK
dc.identifier.eissn1524-4539-
dc.identifier.isiWOS:000275331600008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLian, Q=7003399023en_HK
dc.identifier.scopusauthoridZhang, Y=35785466900en_HK
dc.identifier.scopusauthoridZhang, J=7601360461en_HK
dc.identifier.scopusauthoridZhang, HK=8570008900en_HK
dc.identifier.scopusauthoridWu, X=35785326800en_HK
dc.identifier.scopusauthoridZhang, Y=22954628200en_HK
dc.identifier.scopusauthoridLam, FFY=36783962600en_HK
dc.identifier.scopusauthoridKang, S=35784389200en_HK
dc.identifier.scopusauthoridXia, JC=7402327355en_HK
dc.identifier.scopusauthoridLai, WH=36790434600en_HK
dc.identifier.scopusauthoridAu, KW=9738204200en_HK
dc.identifier.scopusauthoridChow, YY=35784012900en_HK
dc.identifier.scopusauthoridSiu, CW=7006550690en_HK
dc.identifier.scopusauthoridLee, CN=15834991300en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.citeulike6725873-

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