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Article: Gene transcription and synthesis of adrenomedullin by cultured human renal cells

TitleGene transcription and synthesis of adrenomedullin by cultured human renal cells
Authors
KeywordsAdrenomedullin
Glomerular epithelial cells
Human glomerulus
Mesangium
Synthesis
Transcription
Issue Date1998
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 1998, v. 244 n. 2, p. 567-572 How to Cite?
AbstractAdrenomedullin (ADM) is a novel 52 amino acid peptide with a potent vasodilator effect. Gene expression of ADM is found in human kidney but the exact cell source in the kidney is uncertain. Its plasma level is raised in association with changes in sympathetic nervous activity and body fluid volume in hypertension and chronic renal failure. Herein, we examined the presence of mRNA encoding for ADM in cultured human glomerular cells. Adrenomedullin in cell culture supernatant was measured by a radio-immunoassay (with a detection level of 3.2 pmol/l). Adrenomedullin mRNA was found in cultured mesangial and glomerular epithelial cells as well as in vascular endothelial cells. Supernatant levels of ADM for cultured mesangial and glomerular epithelial cells were 21.2 and < 3.2 pmol/l respectively. Contrary to vascular smooth muscle cells, the gene expression for ADM in mesangial cells was up-regulated when incubated with increasing concentration of TNF-α or fetal bovine serum (FBS) but this effect was not observed with very high concentration. Parallel results were observed in adrenomedullin levels in supernatant from mesangial cell cultures. Forskolin, captopril, or TGF-β had no effect on the transcription or synthesis of ADM in mesangial cells. The gene expression for ADM in glomerular epithelial cells was down-regulated when incubated with increasing concentration of TNF-α, forskolin or FBS. The ADM levels in all supernatant from resting glomerular epithelial cell cultures were < 3.2 pmol/l. Recent murine data show that ADM stimulates the release of cAMP but suppresses mitogenesis in cultured mesangial cells. Our results suggest ADM is synthesized by mesangial cells in an autocrine fashion and the peptide may potentially be involved in intra-renal blood pressure control.
Persistent Identifierhttp://hdl.handle.net/10722/78590
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorYeung, VTFen_HK
dc.contributor.authorLewis, LKen_HK
dc.contributor.authorNicholls, MGen_HK
dc.date.accessioned2010-09-06T07:44:33Z-
dc.date.available2010-09-06T07:44:33Z-
dc.date.issued1998en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 1998, v. 244 n. 2, p. 567-572en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/78590-
dc.description.abstractAdrenomedullin (ADM) is a novel 52 amino acid peptide with a potent vasodilator effect. Gene expression of ADM is found in human kidney but the exact cell source in the kidney is uncertain. Its plasma level is raised in association with changes in sympathetic nervous activity and body fluid volume in hypertension and chronic renal failure. Herein, we examined the presence of mRNA encoding for ADM in cultured human glomerular cells. Adrenomedullin in cell culture supernatant was measured by a radio-immunoassay (with a detection level of 3.2 pmol/l). Adrenomedullin mRNA was found in cultured mesangial and glomerular epithelial cells as well as in vascular endothelial cells. Supernatant levels of ADM for cultured mesangial and glomerular epithelial cells were 21.2 and < 3.2 pmol/l respectively. Contrary to vascular smooth muscle cells, the gene expression for ADM in mesangial cells was up-regulated when incubated with increasing concentration of TNF-α or fetal bovine serum (FBS) but this effect was not observed with very high concentration. Parallel results were observed in adrenomedullin levels in supernatant from mesangial cell cultures. Forskolin, captopril, or TGF-β had no effect on the transcription or synthesis of ADM in mesangial cells. The gene expression for ADM in glomerular epithelial cells was down-regulated when incubated with increasing concentration of TNF-α, forskolin or FBS. The ADM levels in all supernatant from resting glomerular epithelial cell cultures were < 3.2 pmol/l. Recent murine data show that ADM stimulates the release of cAMP but suppresses mitogenesis in cultured mesangial cells. Our results suggest ADM is synthesized by mesangial cells in an autocrine fashion and the peptide may potentially be involved in intra-renal blood pressure control.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectAdrenomedullinen_HK
dc.subjectGlomerular epithelial cellsen_HK
dc.subjectHuman glomerulusen_HK
dc.subjectMesangiumen_HK
dc.subjectSynthesisen_HK
dc.subjectTranscriptionen_HK
dc.subject.meshAdrenomedullinen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBlood Pressure - physiologyen_HK
dc.subject.meshCaptopril - pharmacologyen_HK
dc.subject.meshCattleen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCyclic AMP - pharmacologyen_HK
dc.subject.meshDNA Primers - geneticsen_HK
dc.subject.meshEndothelium, Vascular - metabolismen_HK
dc.subject.meshEpithelial Cells - metabolismen_HK
dc.subject.meshForskolin - pharmacologyen_HK
dc.subject.meshGene Expression - drug effectsen_HK
dc.subject.meshGlomerular Mesangium - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKidney - drug effects - metabolism - physiologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshPeptide Biosynthesisen_HK
dc.subject.meshPeptides - genetics - metabolism - physiologyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshRNA, Messenger - genetics - metabolismen_HK
dc.subject.meshTranscription, Genetic - drug effectsen_HK
dc.subject.meshTumor Necrosis Factor-alpha - drug effectsen_HK
dc.titleGene transcription and synthesis of adrenomedullin by cultured human renal cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=244&spage=567&epage=572&date=1998&atitle=Gene+transcription+and+synthesis+of+adrenomedullin+by+cultured+human+renal+cellsen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/bbrc.1998.8167en_HK
dc.identifier.pmid9514865-
dc.identifier.scopuseid_2-s2.0-0032539770en_HK
dc.identifier.hkuros41502en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032539770&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume244en_HK
dc.identifier.issue2en_HK
dc.identifier.spage567en_HK
dc.identifier.epage572en_HK
dc.identifier.isiWOS:000072881200043-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridYeung, VTF=35892061300en_HK
dc.identifier.scopusauthoridLewis, LK=7402152662en_HK
dc.identifier.scopusauthoridNicholls, MG=7202358024en_HK

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