File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1006/bbrc.1998.8167
- Scopus: eid_2-s2.0-0032539770
- PMID: 9514865
- WOS: WOS:000072881200043
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Gene transcription and synthesis of adrenomedullin by cultured human renal cells
Title | Gene transcription and synthesis of adrenomedullin by cultured human renal cells |
---|---|
Authors | |
Keywords | Adrenomedullin Glomerular epithelial cells Human glomerulus Mesangium Synthesis Transcription |
Issue Date | 1998 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description |
Citation | Biochemical And Biophysical Research Communications, 1998, v. 244 n. 2, p. 567-572 How to Cite? |
Abstract | Adrenomedullin (ADM) is a novel 52 amino acid peptide with a potent vasodilator effect. Gene expression of ADM is found in human kidney but the exact cell source in the kidney is uncertain. Its plasma level is raised in association with changes in sympathetic nervous activity and body fluid volume in hypertension and chronic renal failure. Herein, we examined the presence of mRNA encoding for ADM in cultured human glomerular cells. Adrenomedullin in cell culture supernatant was measured by a radio-immunoassay (with a detection level of 3.2 pmol/l). Adrenomedullin mRNA was found in cultured mesangial and glomerular epithelial cells as well as in vascular endothelial cells. Supernatant levels of ADM for cultured mesangial and glomerular epithelial cells were 21.2 and < 3.2 pmol/l respectively. Contrary to vascular smooth muscle cells, the gene expression for ADM in mesangial cells was up-regulated when incubated with increasing concentration of TNF-α or fetal bovine serum (FBS) but this effect was not observed with very high concentration. Parallel results were observed in adrenomedullin levels in supernatant from mesangial cell cultures. Forskolin, captopril, or TGF-β had no effect on the transcription or synthesis of ADM in mesangial cells. The gene expression for ADM in glomerular epithelial cells was down-regulated when incubated with increasing concentration of TNF-α, forskolin or FBS. The ADM levels in all supernatant from resting glomerular epithelial cell cultures were < 3.2 pmol/l. Recent murine data show that ADM stimulates the release of cAMP but suppresses mitogenesis in cultured mesangial cells. Our results suggest ADM is synthesized by mesangial cells in an autocrine fashion and the peptide may potentially be involved in intra-renal blood pressure control. |
Persistent Identifier | http://hdl.handle.net/10722/78590 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.770 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lai, KN | en_HK |
dc.contributor.author | Leung, JCK | en_HK |
dc.contributor.author | Yeung, VTF | en_HK |
dc.contributor.author | Lewis, LK | en_HK |
dc.contributor.author | Nicholls, MG | en_HK |
dc.date.accessioned | 2010-09-06T07:44:33Z | - |
dc.date.available | 2010-09-06T07:44:33Z | - |
dc.date.issued | 1998 | en_HK |
dc.identifier.citation | Biochemical And Biophysical Research Communications, 1998, v. 244 n. 2, p. 567-572 | en_HK |
dc.identifier.issn | 0006-291X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/78590 | - |
dc.description.abstract | Adrenomedullin (ADM) is a novel 52 amino acid peptide with a potent vasodilator effect. Gene expression of ADM is found in human kidney but the exact cell source in the kidney is uncertain. Its plasma level is raised in association with changes in sympathetic nervous activity and body fluid volume in hypertension and chronic renal failure. Herein, we examined the presence of mRNA encoding for ADM in cultured human glomerular cells. Adrenomedullin in cell culture supernatant was measured by a radio-immunoassay (with a detection level of 3.2 pmol/l). Adrenomedullin mRNA was found in cultured mesangial and glomerular epithelial cells as well as in vascular endothelial cells. Supernatant levels of ADM for cultured mesangial and glomerular epithelial cells were 21.2 and < 3.2 pmol/l respectively. Contrary to vascular smooth muscle cells, the gene expression for ADM in mesangial cells was up-regulated when incubated with increasing concentration of TNF-α or fetal bovine serum (FBS) but this effect was not observed with very high concentration. Parallel results were observed in adrenomedullin levels in supernatant from mesangial cell cultures. Forskolin, captopril, or TGF-β had no effect on the transcription or synthesis of ADM in mesangial cells. The gene expression for ADM in glomerular epithelial cells was down-regulated when incubated with increasing concentration of TNF-α, forskolin or FBS. The ADM levels in all supernatant from resting glomerular epithelial cell cultures were < 3.2 pmol/l. Recent murine data show that ADM stimulates the release of cAMP but suppresses mitogenesis in cultured mesangial cells. Our results suggest ADM is synthesized by mesangial cells in an autocrine fashion and the peptide may potentially be involved in intra-renal blood pressure control. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description | en_HK |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | en_HK |
dc.subject | Adrenomedullin | en_HK |
dc.subject | Glomerular epithelial cells | en_HK |
dc.subject | Human glomerulus | en_HK |
dc.subject | Mesangium | en_HK |
dc.subject | Synthesis | en_HK |
dc.subject | Transcription | en_HK |
dc.subject.mesh | Adrenomedullin | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Base Sequence | en_HK |
dc.subject.mesh | Blood Pressure - physiology | en_HK |
dc.subject.mesh | Captopril - pharmacology | en_HK |
dc.subject.mesh | Cattle | en_HK |
dc.subject.mesh | Cells, Cultured | en_HK |
dc.subject.mesh | Cyclic AMP - pharmacology | en_HK |
dc.subject.mesh | DNA Primers - genetics | en_HK |
dc.subject.mesh | Endothelium, Vascular - metabolism | en_HK |
dc.subject.mesh | Epithelial Cells - metabolism | en_HK |
dc.subject.mesh | Forskolin - pharmacology | en_HK |
dc.subject.mesh | Gene Expression - drug effects | en_HK |
dc.subject.mesh | Glomerular Mesangium - metabolism | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Kidney - drug effects - metabolism - physiology | en_HK |
dc.subject.mesh | Mice | en_HK |
dc.subject.mesh | Peptide Biosynthesis | en_HK |
dc.subject.mesh | Peptides - genetics - metabolism - physiology | en_HK |
dc.subject.mesh | Polymerase Chain Reaction | en_HK |
dc.subject.mesh | RNA, Messenger - genetics - metabolism | en_HK |
dc.subject.mesh | Transcription, Genetic - drug effects | en_HK |
dc.subject.mesh | Tumor Necrosis Factor-alpha - drug effects | en_HK |
dc.title | Gene transcription and synthesis of adrenomedullin by cultured human renal cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=244&spage=567&epage=572&date=1998&atitle=Gene+transcription+and+synthesis+of+adrenomedullin+by+cultured+human+renal+cells | en_HK |
dc.identifier.email | Lai, KN: knlai@hku.hk | en_HK |
dc.identifier.email | Leung, JCK: jckleung@hku.hk | en_HK |
dc.identifier.authority | Lai, KN=rp00324 | en_HK |
dc.identifier.authority | Leung, JCK=rp00448 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1006/bbrc.1998.8167 | en_HK |
dc.identifier.pmid | 9514865 | - |
dc.identifier.scopus | eid_2-s2.0-0032539770 | en_HK |
dc.identifier.hkuros | 41502 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0032539770&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 244 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 567 | en_HK |
dc.identifier.epage | 572 | en_HK |
dc.identifier.isi | WOS:000072881200043 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Lai, KN=7402135706 | en_HK |
dc.identifier.scopusauthorid | Leung, JCK=7202180349 | en_HK |
dc.identifier.scopusauthorid | Yeung, VTF=35892061300 | en_HK |
dc.identifier.scopusauthorid | Lewis, LK=7402152662 | en_HK |
dc.identifier.scopusauthorid | Nicholls, MG=7202358024 | en_HK |
dc.identifier.issnl | 0006-291X | - |