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Article: A semi-automated method for analysis of intron 13 and intron 22 dinucleotide repeat polymorphisms of the factor VIII gene

TitleA semi-automated method for analysis of intron 13 and intron 22 dinucleotide repeat polymorphisms of the factor VIII gene
Authors
Issue Date1996
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLH
Citation
Clinical And Laboratory Haematology, 1996, v. 18 n. 2, p. 111-114 How to Cite?
AbstractA semi-automated method has been developed for the simultaneous analysis of two microsatellite repeat polymorphisms located in intron 13 and 22 of the Factor VIII gene. The fluorescent dyes, 6-FAM- and HEX- phosphoramidites were used to 5'-end label the respective 5' primers of these two microsatellite repeats and a multiplex polymerase chain reaction (PCR) devised for amplification. The PCR product was loaded onto the gel with DNA size marker labelled with ROX. A rural of 24 samples could be analysed simultaneously on an automated DNA sequencer. The results were computed using a dedicated software, with assignment of PCR product size in basepair. This method compares well with the conventional manual procedure using radio-labelled primers, but at the same time overcomes many of the inherent disadvantages associated with the latter method.
Persistent Identifierhttp://hdl.handle.net/10722/78578
ISSN
2008 Impact Factor: 1.304
2015 SCImago Journal Rankings: 0.654
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYip, Ben_HK
dc.contributor.authorChan, Ven_HK
dc.contributor.authorChan, TKen_HK
dc.date.accessioned2010-09-06T07:44:25Z-
dc.date.available2010-09-06T07:44:25Z-
dc.date.issued1996en_HK
dc.identifier.citationClinical And Laboratory Haematology, 1996, v. 18 n. 2, p. 111-114en_HK
dc.identifier.issn0141-9854en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78578-
dc.description.abstractA semi-automated method has been developed for the simultaneous analysis of two microsatellite repeat polymorphisms located in intron 13 and 22 of the Factor VIII gene. The fluorescent dyes, 6-FAM- and HEX- phosphoramidites were used to 5'-end label the respective 5' primers of these two microsatellite repeats and a multiplex polymerase chain reaction (PCR) devised for amplification. The PCR product was loaded onto the gel with DNA size marker labelled with ROX. A rural of 24 samples could be analysed simultaneously on an automated DNA sequencer. The results were computed using a dedicated software, with assignment of PCR product size in basepair. This method compares well with the conventional manual procedure using radio-labelled primers, but at the same time overcomes many of the inherent disadvantages associated with the latter method.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLHen_HK
dc.relation.ispartofClinical and Laboratory Haematologyen_HK
dc.rightsClinical and Laboratory Haematology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subject.meshAllelesen_HK
dc.subject.meshAutomationen_HK
dc.subject.meshDNA Mutational Analysis - methodsen_HK
dc.subject.meshDNA Primersen_HK
dc.subject.meshDinucleotide Repeatsen_HK
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_HK
dc.subject.meshFactor VIII - geneticsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFluorescent Dyesen_HK
dc.subject.meshGenotypeen_HK
dc.subject.meshHaplotypes - geneticsen_HK
dc.subject.meshHemophilia A - blood - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIntrons - geneticsen_HK
dc.subject.meshLeukocytes - chemistryen_HK
dc.subject.meshMaleen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshSoftwareen_HK
dc.titleA semi-automated method for analysis of intron 13 and intron 22 dinucleotide repeat polymorphisms of the factor VIII geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0141-9854&volume=18&spage=4pp&epage=&date=1995&atitle=A+semi-automated+method+for+analysis+of+intron+13+and+intron+22+dinucleotide+repeat+polymorphisms+of+the+factor+VIII+geneen_HK
dc.identifier.emailChan, V:vnychana@hkucc.hku.hken_HK
dc.identifier.authorityChan, V=rp00320en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1365-2257.1996.00163.x-
dc.identifier.pmid8866144en_HK
dc.identifier.scopuseid_2-s2.0-0029780388en_HK
dc.identifier.hkuros12279en_HK
dc.identifier.hkuros22984-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029780388&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue2en_HK
dc.identifier.spage111en_HK
dc.identifier.epage114en_HK
dc.identifier.isiWOS:A1996VC72200009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYip, B=16685586100en_HK
dc.identifier.scopusauthoridChan, V=7202654865en_HK
dc.identifier.scopusauthoridChan, TK=7402687762en_HK

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