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Article: Relationship of expression of aquaglyceroporin 9 with arsenic uptake and sensitivity in leukemia cells

TitleRelationship of expression of aquaglyceroporin 9 with arsenic uptake and sensitivity in leukemia cells
Authors
Issue Date2007
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 2007, v. 109 n. 2, p. 740-746 How to Cite?
AbstractArsenic trioxide (As 2O 3) is highly efficacious in acute promyelocytic leukemia (APL). Aquaglyceroporin 9 (AQP9) is a transmembrane protein that may be involved in arsenic uptake. In 10 of 11 myeloid and lymphoid leukemia lines, quantitative polymerase chain reaction (Q-PCR) and Western blotting showed that AQP9 expression correlated positively with As 2O 3-induced cytotoxicity. As a proof-of-principle, transfection of EGFP-tagged AQP9 to the hepatoma line Hep3B, not expressing AQP9 and As 2O 3 insensitive, led to membrane AQP9 expression and increased As 2O 3-induced cytotoxicity. Similarly, the chronic myeloid leukemia line K562 expressed low levels of AQP9 and was As 2O 3 insensitive. The K562 EGFP-AQP9 transfectant accumulated significantly higher levels of intracellular arsenic than control K562 EGFP when incubated with As 2O 3, resulting in significantly increased As 2O 3-induced cytotoxicity. Pretreatment of the myeloid leukemia line HL-60 with all-trans retinoic acid (ATRA) up-regulated AQP9, leading to a significantly increased arsenic uptake and As 2O 3-induced cytotoxicity on incubation with As 2O 3, which might explain the synergism between ATRA and As 2O 3. Therefore, AQP9 controlled arsenic transport and might determine As 2O 3 sensitivity. Q-PCR showed that primary APL cells expressed AQP9 significantly (2-3 logs) higher than other acute myeloid leukemias (AMLs), which might explain their exquisite As 2O 3 sensitivity. However, APL and AML with maturation expressed comparable AQP9 levels, suggesting that AQP9 expression was related to granulocytic maturation. © 2007 by The American Society of Hematology.
Persistent Identifierhttp://hdl.handle.net/10722/78570
ISSN
2015 Impact Factor: 11.841
2015 SCImago Journal Rankings: 6.395
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, Jen_HK
dc.contributor.authorPang, Aen_HK
dc.contributor.authorYuen, WHen_HK
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorTse, EWCen_HK
dc.date.accessioned2010-09-06T07:44:20Z-
dc.date.available2010-09-06T07:44:20Z-
dc.date.issued2007en_HK
dc.identifier.citationBlood, 2007, v. 109 n. 2, p. 740-746en_HK
dc.identifier.issn0006-4971en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78570-
dc.description.abstractArsenic trioxide (As 2O 3) is highly efficacious in acute promyelocytic leukemia (APL). Aquaglyceroporin 9 (AQP9) is a transmembrane protein that may be involved in arsenic uptake. In 10 of 11 myeloid and lymphoid leukemia lines, quantitative polymerase chain reaction (Q-PCR) and Western blotting showed that AQP9 expression correlated positively with As 2O 3-induced cytotoxicity. As a proof-of-principle, transfection of EGFP-tagged AQP9 to the hepatoma line Hep3B, not expressing AQP9 and As 2O 3 insensitive, led to membrane AQP9 expression and increased As 2O 3-induced cytotoxicity. Similarly, the chronic myeloid leukemia line K562 expressed low levels of AQP9 and was As 2O 3 insensitive. The K562 EGFP-AQP9 transfectant accumulated significantly higher levels of intracellular arsenic than control K562 EGFP when incubated with As 2O 3, resulting in significantly increased As 2O 3-induced cytotoxicity. Pretreatment of the myeloid leukemia line HL-60 with all-trans retinoic acid (ATRA) up-regulated AQP9, leading to a significantly increased arsenic uptake and As 2O 3-induced cytotoxicity on incubation with As 2O 3, which might explain the synergism between ATRA and As 2O 3. Therefore, AQP9 controlled arsenic transport and might determine As 2O 3 sensitivity. Q-PCR showed that primary APL cells expressed AQP9 significantly (2-3 logs) higher than other acute myeloid leukemias (AMLs), which might explain their exquisite As 2O 3 sensitivity. However, APL and AML with maturation expressed comparable AQP9 levels, suggesting that AQP9 expression was related to granulocytic maturation. © 2007 by The American Society of Hematology.en_HK
dc.languageengen_HK
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/en_HK
dc.relation.ispartofBlooden_HK
dc.subject.meshAcute Diseaseen_HK
dc.subject.meshAquaporins - drug effects - genetics - metabolismen_HK
dc.subject.meshArsenicals - metabolism - pharmacokinetics - pharmacologyen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshCell Proliferation - drug effectsen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshHumansen_HK
dc.subject.meshK562 Cellsen_HK
dc.subject.meshLeukemia, Myeloid - metabolismen_HK
dc.subject.meshLeukemia, Promyelocytic, Acute - drug therapy - metabolismen_HK
dc.subject.meshOxides - metabolism - pharmacokinetics - pharmacologyen_HK
dc.subject.meshPoint Mutationen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - methodsen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshTretinoin - pharmacologyen_HK
dc.subject.meshUp-Regulation - drug effectsen_HK
dc.titleRelationship of expression of aquaglyceroporin 9 with arsenic uptake and sensitivity in leukemia cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-4971&volume=109&spage=740&epage=746&date=2007&atitle=Relationship+of+Expression+of+Aquaglyceroporin+9+with+Arsenic+Uptake+and+Sensitivity+in+Leukemia+Cells+en_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.emailTse, EWC:ewctse@hku.hken_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.authorityTse, EWC=rp00471en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1182/blood-2006-04-019588en_HK
dc.identifier.pmid16968895-
dc.identifier.scopuseid_2-s2.0-33846233015en_HK
dc.identifier.hkuros131487en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846233015&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume109en_HK
dc.identifier.issue2en_HK
dc.identifier.spage740en_HK
dc.identifier.epage746en_HK
dc.identifier.eissn1528-0020-
dc.identifier.isiWOS:000243416600054-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLeung, J=53869381100en_HK
dc.identifier.scopusauthoridPang, A=7007044165en_HK
dc.identifier.scopusauthoridYuen, WH=7102761282en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridTse, EWC=7005019454en_HK

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