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Article: Regulation of primitive hematopoiesis in zebrafish embryos by the death receptor gene

TitleRegulation of primitive hematopoiesis in zebrafish embryos by the death receptor gene
Authors
Issue Date2006
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphem
Citation
Experimental Hematology, 2006, v. 34 n. 1, p. 27-34 How to Cite?
AbstractObjective. We investigated the regulatory mechanism of primitive hematopoiesis in zebrafish (Danio rerio) embryos with particular reference to the role of a death receptor (zDR) gene, based on a morpholino (MO) knockdown approach. Methods. MOs targeting the zDR and chordin (Chd) were injected into naturally spawned embryos at one- to four-cell stage. A random sequence (RS) MO was used as a control. Effects on hemoglobin formation (Hb), apoptosis, and lineage-specific gene expression were examined. Embryos injected with zDR, Chd, and RS-MOs were denoted zDR mo, zChd mo, and zRS mo, respectively. Those coinjected with Chd+zDR-MOs and Chd+RS-MOs were abbreviated zChd+DR mo and zChd+RS mo. Results. zDR mRNA expression was restricted to the intermediate cell mass of wild-type (WT) and zChd mo embryos. At 48 hours postfertilization, zDR mo embryos showed increased Hb compared with WT or zRS mo embryos (2.36 × 10 -2 ± 1.13 × 10 -3 vs 1.85 × 10 -2 ± 5.60 × 10 -4 vs 1.79 × 10 -2 ± 1.31 × 10 -3 U, p < 0.05). zChd+DR mo embryos also showed increased Hb compared with zChd mo or zChd+RS mo embryos (4.60 × 10 -2 ± 2.79 × 10 -3 vs 3.17 × 10 -2 ± 1.07 × 10 -3 vs 3.05 × 10 -2 ± 1.25 × 10 -3 U, p < 0.05). zDR-MO reduced apoptosis, as shown by reduced terminal transferase-mediated dUTP nick end-labeling staining in zChd+DR mo compared with zChd+RS mo embryos and caspase-3 activity in zDR mo vs zRS mo (0.525 ± 0.094 vs 0.953 ± 0.113 U, p < 0.05), and zChd+DR mo vs zChd+RS mo embryos (0.247 ± 0.121 vs 1.180 ± 0.082, p < 0.05). zChd+DR mo embryos showed upregulation of erythroid-specific embryonic hemoglobin gene expression but not that of a myeloid-specific myeloperoxidase gene. Conclusion. Knockdown of zDR in zebrafish embryos decreased apoptosis and increased Hb, suggesting that zDR may regulate primitive hematopoiesis during development. © 2006 International Society for Experimental Hematology. Published by Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/78487
ISSN
2015 Impact Factor: 2.303
2015 SCImago Journal Rankings: 1.341
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKwan, TTen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorVerfaillie, CMen_HK
dc.contributor.authorEkker, SCen_HK
dc.contributor.authorChan, LCen_HK
dc.contributor.authorLin, Sen_HK
dc.contributor.authorLeung, AYHen_HK
dc.date.accessioned2010-09-06T07:43:26Z-
dc.date.available2010-09-06T07:43:26Z-
dc.date.issued2006en_HK
dc.identifier.citationExperimental Hematology, 2006, v. 34 n. 1, p. 27-34en_HK
dc.identifier.issn0301-472Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/78487-
dc.description.abstractObjective. We investigated the regulatory mechanism of primitive hematopoiesis in zebrafish (Danio rerio) embryos with particular reference to the role of a death receptor (zDR) gene, based on a morpholino (MO) knockdown approach. Methods. MOs targeting the zDR and chordin (Chd) were injected into naturally spawned embryos at one- to four-cell stage. A random sequence (RS) MO was used as a control. Effects on hemoglobin formation (Hb), apoptosis, and lineage-specific gene expression were examined. Embryos injected with zDR, Chd, and RS-MOs were denoted zDR mo, zChd mo, and zRS mo, respectively. Those coinjected with Chd+zDR-MOs and Chd+RS-MOs were abbreviated zChd+DR mo and zChd+RS mo. Results. zDR mRNA expression was restricted to the intermediate cell mass of wild-type (WT) and zChd mo embryos. At 48 hours postfertilization, zDR mo embryos showed increased Hb compared with WT or zRS mo embryos (2.36 × 10 -2 ± 1.13 × 10 -3 vs 1.85 × 10 -2 ± 5.60 × 10 -4 vs 1.79 × 10 -2 ± 1.31 × 10 -3 U, p < 0.05). zChd+DR mo embryos also showed increased Hb compared with zChd mo or zChd+RS mo embryos (4.60 × 10 -2 ± 2.79 × 10 -3 vs 3.17 × 10 -2 ± 1.07 × 10 -3 vs 3.05 × 10 -2 ± 1.25 × 10 -3 U, p < 0.05). zDR-MO reduced apoptosis, as shown by reduced terminal transferase-mediated dUTP nick end-labeling staining in zChd+DR mo compared with zChd+RS mo embryos and caspase-3 activity in zDR mo vs zRS mo (0.525 ± 0.094 vs 0.953 ± 0.113 U, p < 0.05), and zChd+DR mo vs zChd+RS mo embryos (0.247 ± 0.121 vs 1.180 ± 0.082, p < 0.05). zChd+DR mo embryos showed upregulation of erythroid-specific embryonic hemoglobin gene expression but not that of a myeloid-specific myeloperoxidase gene. Conclusion. Knockdown of zDR in zebrafish embryos decreased apoptosis and increased Hb, suggesting that zDR may regulate primitive hematopoiesis during development. © 2006 International Society for Experimental Hematology. Published by Elsevier Inc.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphemen_HK
dc.relation.ispartofExperimental Hematologyen_HK
dc.rightsExperimental Hematology . Copyright © Elsevier Inc.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshApoptosis - drug effectsen_HK
dc.subject.meshCaspase 3en_HK
dc.subject.meshCaspases - drug effects - metabolismen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshEmbryo, Nonmammalianen_HK
dc.subject.meshGene Expression Regulation, Developmental - drug effectsen_HK
dc.subject.meshGlycoproteins - administration & dosageen_HK
dc.subject.meshHematopoiesis - drug effects - genetics - physiologyen_HK
dc.subject.meshHemoglobins - biosynthesis - drug effectsen_HK
dc.subject.meshIntercellular Signaling Peptides and Proteins - administration & dosageen_HK
dc.subject.meshMorpholines - administration & dosageen_HK
dc.subject.meshReceptors, Tumor Necrosis Factor - administration & dosage - genetics - metabolismen_HK
dc.subject.meshZebrafish - embryology - genetics - metabolismen_HK
dc.titleRegulation of primitive hematopoiesis in zebrafish embryos by the death receptor geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-472X&volume=34&issue=1&spage=27&epage=34&date=2006&atitle=Regulation+of+primitive+hematopoiesis+in+zebrafish+embryos+by+the+death+receptor+geneen_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_HK
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.exphem.2005.09.017en_HK
dc.identifier.pmid16413388-
dc.identifier.scopuseid_2-s2.0-30344457882en_HK
dc.identifier.hkuros122751en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-30344457882&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume34en_HK
dc.identifier.issue1en_HK
dc.identifier.spage27en_HK
dc.identifier.epage34en_HK
dc.identifier.isiWOS:000235133900005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKwan, TT=8776810800en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridVerfaillie, CM=7004524257en_HK
dc.identifier.scopusauthoridEkker, SC=7003541150en_HK
dc.identifier.scopusauthoridChan, LC=7403540707en_HK
dc.identifier.scopusauthoridLin, S=7407161307en_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK

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