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Article: Neuropeptide Y-Y1 receptor agonist worsens while antagonist improves survival of cultured Y1-expressing neuronal cells following oxygen and glucose deprivation

TitleNeuropeptide Y-Y1 receptor agonist worsens while antagonist improves survival of cultured Y1-expressing neuronal cells following oxygen and glucose deprivation
Authors
Issue Date2004
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1021-7770
Citation
Journal Of Biomedical Science, 2004, v. 11 n. 6, p. 781-788 How to Cite?
AbstractIn this in vitro study, we investigated the influence of neuropeptide Y (NPY) Y1 receptor activation or inhibition on the viability of cultured neuronal or glial cells following oxygen glucose deprivation (OGD). Viability of cultured cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide. When compared to the vehicle-treated control group, treatment with NPY or [Leu31,Pro34]-NPY (Y1 agonist) reduced viability of cultured SK-N-MC (Y1-expressing) human neuronal cells at 24 h after 1 h of OGD, while BIBP3226 (Y1 antagonist) improved viability. Except at the highest concentration of NPY used in the study, treatment with NPY or NPY3-36 (Y2 agonist) did not influence viability of cultured SH-SY5Y (Y2-expressing) human neuronal cells at 24 h after 1 h of OGD. In addition, treatment with NPY, [Leu31,Pro34]-NPY, NPY3-36, or BIBP3226 did not affect viability of cultured primary astrocytes at 24 h after 4 h of OGD. The present results agree with those of a recent in vivo study. Activation of NPY-Y1 receptors may mediate ischemic pathophysiological processes, and inhibiting the Y1 receptors may be protective. The combination of OGD and cultured neuronal cells may be useful in future studies on the neuroprotective and harmful mechanisms of NPY-Y1 receptor inhibition and activation during ischemia, respectively. Copyright © 2004 National Science Council, ROC and S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/78483
ISSN
2015 Impact Factor: 2.935
2015 SCImago Journal Rankings: 1.280
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, SHen_HK
dc.contributor.authorCheung, RTFen_HK
dc.date.accessioned2010-09-06T07:43:23Z-
dc.date.available2010-09-06T07:43:23Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Biomedical Science, 2004, v. 11 n. 6, p. 781-788en_HK
dc.identifier.issn1021-7770en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78483-
dc.description.abstractIn this in vitro study, we investigated the influence of neuropeptide Y (NPY) Y1 receptor activation or inhibition on the viability of cultured neuronal or glial cells following oxygen glucose deprivation (OGD). Viability of cultured cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide. When compared to the vehicle-treated control group, treatment with NPY or [Leu31,Pro34]-NPY (Y1 agonist) reduced viability of cultured SK-N-MC (Y1-expressing) human neuronal cells at 24 h after 1 h of OGD, while BIBP3226 (Y1 antagonist) improved viability. Except at the highest concentration of NPY used in the study, treatment with NPY or NPY3-36 (Y2 agonist) did not influence viability of cultured SH-SY5Y (Y2-expressing) human neuronal cells at 24 h after 1 h of OGD. In addition, treatment with NPY, [Leu31,Pro34]-NPY, NPY3-36, or BIBP3226 did not affect viability of cultured primary astrocytes at 24 h after 4 h of OGD. The present results agree with those of a recent in vivo study. Activation of NPY-Y1 receptors may mediate ischemic pathophysiological processes, and inhibiting the Y1 receptors may be protective. The combination of OGD and cultured neuronal cells may be useful in future studies on the neuroprotective and harmful mechanisms of NPY-Y1 receptor inhibition and activation during ischemia, respectively. Copyright © 2004 National Science Council, ROC and S. Karger AG, Basel.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1021-7770en_HK
dc.relation.ispartofJournal of Biomedical Scienceen_HK
dc.subject.meshAnti-Anxiety Agents - pharmacologyen_HK
dc.subject.meshArginine - analogs & derivatives - pharmacologyen_HK
dc.subject.meshAstrocytes - metabolismen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCell Survivalen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshColoring Agents - pharmacologyen_HK
dc.subject.meshGlucose - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIschemiaen_HK
dc.subject.meshNeuroglia - metabolismen_HK
dc.subject.meshNeurons - metabolismen_HK
dc.subject.meshNeuropeptide Y - biosynthesisen_HK
dc.subject.meshNeuroprotective Agents - pharmacologyen_HK
dc.subject.meshOxygen - metabolismen_HK
dc.subject.meshReceptors, Neuropeptide Y - agonists - antagonists & inhibitors - metabolismen_HK
dc.subject.meshReperfusion Injuryen_HK
dc.subject.meshTetrazolium Salts - pharmacologyen_HK
dc.subject.meshThiazoles - pharmacologyen_HK
dc.subject.meshTime Factorsen_HK
dc.titleNeuropeptide Y-Y1 receptor agonist worsens while antagonist improves survival of cultured Y1-expressing neuronal cells following oxygen and glucose deprivationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1021-7770&volume=11&spage=781&epage=788&date=2004&atitle=Neuropeptide+Y-Y1+receptor+agonist+worsens+while+antagonist+improves+survival+of+cultured+Y1-expressing+neuronal+cells+following+oxygen+and+glucose+deprivationen_HK
dc.identifier.emailCheung, RTF:rtcheung@hku.hken_HK
dc.identifier.authorityCheung, RTF=rp00434en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000081825en_HK
dc.identifier.pmid15591775-
dc.identifier.scopuseid_2-s2.0-10844274568en_HK
dc.identifier.hkuros99209en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-10844274568&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue6en_HK
dc.identifier.spage781en_HK
dc.identifier.epage788en_HK
dc.identifier.isiWOS:000225547000010-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, SH=12806098400en_HK
dc.identifier.scopusauthoridCheung, RTF=7202397498en_HK

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