File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Molecular cytogenetic characterization of the 11q13 amplicon in head and neck squamous cell carcinoma

TitleMolecular cytogenetic characterization of the 11q13 amplicon in head and neck squamous cell carcinoma
Authors
Issue Date2006
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/CGR
Citation
Cytogenetic And Genome Research, 2006, v. 115 n. 2, p. 99-106 How to Cite?
AbstractAmplification of 11q13 DNA sequences and overexpression of CCND1 are common findings in head and neck squamous cell carcinoma (HNSCC), identified in about 30% of the cases. However, little is known about initiation of the amplification and the organization of the amplicon. In order to study the structure of the amplicon in more detail and to learn more about the mechanisms involved in its initiation, prometaphase, metaphase, and anaphase fluorescence in situ hybridization (FISH) with 40 BAC clones spanning a 16-Mb region in chromosome bands 11q12.2 to 11q13.5 was performed in nine HNSCC cell lines with homogeneously staining regions. FISH analysis showed that the size of the amplicon varied among the nine cell lines, the smallest being 2.12 Mb and the largest 8.97 Mb. The smallest overlapping region of amplification was approximately 1.61 Mb, covering the region from BAC 729E14 to BAC 102B19. This region contained several genes previously shown to be amplified and overexpressed in HNSCC, including CCDN1, CTTN, SHANK2, and ORAOV1. The cell lines were also used to study the internal structure of the amplicon. Various patterns of amplified DNA sequences within the amplicon were found among the nine cell lines. Even within the same cell line, different amplicon structures could be found in different cell populations, indicating that the mechanisms involved in the development of the amplicons in HNSCC were more complex than previously assumed. The frequent finding of inverted repeats within the amplicons, however, suggests that breakage-fusion-bridge cycles are important in the initiation, but the fact that such repeats constituted only small parts of the amplicons indicate that they are further rearranged during tumor progression. Copyright © 2006 S. Karger AG.
Persistent Identifierhttp://hdl.handle.net/10722/78476
ISSN
2015 Impact Factor: 1.638
2015 SCImago Journal Rankings: 0.744
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, Cen_HK
dc.contributor.authorJin, Yen_HK
dc.contributor.authorGisselsson, Den_HK
dc.contributor.authorWennerberg, Jen_HK
dc.contributor.authorWah, TSen_HK
dc.contributor.authorStrömbäck, Ben_HK
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorMertens, Fen_HK
dc.date.accessioned2010-09-06T07:43:19Z-
dc.date.available2010-09-06T07:43:19Z-
dc.date.issued2006en_HK
dc.identifier.citationCytogenetic And Genome Research, 2006, v. 115 n. 2, p. 99-106en_HK
dc.identifier.issn1424-8581en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78476-
dc.description.abstractAmplification of 11q13 DNA sequences and overexpression of CCND1 are common findings in head and neck squamous cell carcinoma (HNSCC), identified in about 30% of the cases. However, little is known about initiation of the amplification and the organization of the amplicon. In order to study the structure of the amplicon in more detail and to learn more about the mechanisms involved in its initiation, prometaphase, metaphase, and anaphase fluorescence in situ hybridization (FISH) with 40 BAC clones spanning a 16-Mb region in chromosome bands 11q12.2 to 11q13.5 was performed in nine HNSCC cell lines with homogeneously staining regions. FISH analysis showed that the size of the amplicon varied among the nine cell lines, the smallest being 2.12 Mb and the largest 8.97 Mb. The smallest overlapping region of amplification was approximately 1.61 Mb, covering the region from BAC 729E14 to BAC 102B19. This region contained several genes previously shown to be amplified and overexpressed in HNSCC, including CCDN1, CTTN, SHANK2, and ORAOV1. The cell lines were also used to study the internal structure of the amplicon. Various patterns of amplified DNA sequences within the amplicon were found among the nine cell lines. Even within the same cell line, different amplicon structures could be found in different cell populations, indicating that the mechanisms involved in the development of the amplicons in HNSCC were more complex than previously assumed. The frequent finding of inverted repeats within the amplicons, however, suggests that breakage-fusion-bridge cycles are important in the initiation, but the fact that such repeats constituted only small parts of the amplicons indicate that they are further rearranged during tumor progression. Copyright © 2006 S. Karger AG.en_HK
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/CGRen_HK
dc.relation.ispartofCytogenetic and Genome Researchen_HK
dc.rightsCytogenetic and genome research. Copyright © S Karger AG.en_HK
dc.subject.meshAnaphaseen_HK
dc.subject.meshCarcinoma, Squamous Cell - geneticsen_HK
dc.subject.meshCell Line, Tumor - ultrastructureen_HK
dc.subject.meshChromosome Bandingen_HK
dc.subject.meshChromosome Breakageen_HK
dc.subject.meshChromosomes, Artificial, Bacterialen_HK
dc.subject.meshChromosomes, Human, Pair 11 - genetics - ultrastructureen_HK
dc.subject.meshDNA Repairen_HK
dc.subject.meshDNA, Neoplasm - geneticsen_HK
dc.subject.meshDisease Progressionen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Amplificationen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshHead and Neck Neoplasms - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIn Situ Hybridization, Fluorescenceen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMetaphaseen_HK
dc.subject.meshRepetitive Sequences, Nucleic Aciden_HK
dc.titleMolecular cytogenetic characterization of the 11q13 amplicon in head and neck squamous cell carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1424-8581&volume=115&issue=2&spage=99&epage=106&date=2006&atitle=Molecular+cytogenetic+characterization+of+the+11q13+amplicon+in+head+and+neck+squamous+cell+carcinomaen_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000095228en_HK
dc.identifier.pmid17065789-
dc.identifier.scopuseid_2-s2.0-33750362122en_HK
dc.identifier.hkuros146328en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33750362122&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume115en_HK
dc.identifier.issue2en_HK
dc.identifier.spage99en_HK
dc.identifier.epage106en_HK
dc.identifier.isiWOS:000241664700001-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridJin, C=7401659093en_HK
dc.identifier.scopusauthoridJin, Y=7404457413en_HK
dc.identifier.scopusauthoridGisselsson, D=7003453988en_HK
dc.identifier.scopusauthoridWennerberg, J=7007085924en_HK
dc.identifier.scopusauthoridWah, TS=55241392300en_HK
dc.identifier.scopusauthoridStrömbäck, B=6504383631en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridMertens, F=7101611996en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats