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Article: Association of CTLA-4 gene microsatellite polymorphism with ulcerative colitis in Chinese patients

TitleAssociation of CTLA-4 gene microsatellite polymorphism with ulcerative colitis in Chinese patients
Authors
Issue Date2006
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.ibdjournal.com
Citation
Inflammatory Bowel Diseases, 2006, v. 12 n. 5, p. 369-373 How to Cite?
AbstractUlcerative colitis (UC) is characterized by chronic intestinal inflammation as a result of an exaggerated T cell response. Cytotoxic T lymphocyte associated antigen-4 (CTLA-4), expressed mainly in activated T cells, inhibits T cell activation and proliferation by combining B7 through competing CD28 and maintains immune homeostasis. Polymorphisms of the CTLA-4 gene are known to be associated with several autoimmune diseases. The aim of this study was to investigate the association between the CTLA-4 gene micro satellite polymorphism and UC in Chinese patients. Unrelated 100 Chinese patients with UC and 140 healthy controls were studied. The (AT) repeats in the 3′ untranslated region of exon 4 of the CTLA-4 gene were amplified by allele-specific polymerase chain reaction (PCR). The amplified products were electrophoresed on a 12% polyacrylamide gel, followed by silver staining. Twenty alleles were found in Chinese patients and healthy controls. The 122-bp allele was increased in UC compared with healthy controls (9.5% vs 0.7%, P = 0.0001/Pc = 0.002, OR = 14.591, 95% CI 3.357-63.420). The frequency of the longer alleles (≥118 bp) of UC was higher than that in healthy controls (26% vs 4%, P = 0.0001/Pc = 0.0002, OR = 7.644, 95%CI 3.950-14.792), but was not associated with location and severity of the disease. Furthermore, the longer alleles were not associated with haplotypes of C-318T/A+49G of the CTLA-4 gene in Chinese patients with UC. The longer alleles of the CTLA-4 gene microsatellite polymorphism were strongly associated with UC in Chinese patients. Copyright © 2006 by Lippincott Williams & Wilkins.
Persistent Identifierhttp://hdl.handle.net/10722/78391
ISSN
2015 Impact Factor: 4.358
2015 SCImago Journal Rankings: 1.994
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJiang, Yen_HK
dc.contributor.authorXia, Ben_HK
dc.contributor.authorJiang, Len_HK
dc.contributor.authorLv, Men_HK
dc.contributor.authorGuo, Qen_HK
dc.contributor.authorChen, Men_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorXia, HHXen_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2010-09-06T07:42:22Z-
dc.date.available2010-09-06T07:42:22Z-
dc.date.issued2006en_HK
dc.identifier.citationInflammatory Bowel Diseases, 2006, v. 12 n. 5, p. 369-373en_HK
dc.identifier.issn1078-0998en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78391-
dc.description.abstractUlcerative colitis (UC) is characterized by chronic intestinal inflammation as a result of an exaggerated T cell response. Cytotoxic T lymphocyte associated antigen-4 (CTLA-4), expressed mainly in activated T cells, inhibits T cell activation and proliferation by combining B7 through competing CD28 and maintains immune homeostasis. Polymorphisms of the CTLA-4 gene are known to be associated with several autoimmune diseases. The aim of this study was to investigate the association between the CTLA-4 gene micro satellite polymorphism and UC in Chinese patients. Unrelated 100 Chinese patients with UC and 140 healthy controls were studied. The (AT) repeats in the 3′ untranslated region of exon 4 of the CTLA-4 gene were amplified by allele-specific polymerase chain reaction (PCR). The amplified products were electrophoresed on a 12% polyacrylamide gel, followed by silver staining. Twenty alleles were found in Chinese patients and healthy controls. The 122-bp allele was increased in UC compared with healthy controls (9.5% vs 0.7%, P = 0.0001/Pc = 0.002, OR = 14.591, 95% CI 3.357-63.420). The frequency of the longer alleles (≥118 bp) of UC was higher than that in healthy controls (26% vs 4%, P = 0.0001/Pc = 0.0002, OR = 7.644, 95%CI 3.950-14.792), but was not associated with location and severity of the disease. Furthermore, the longer alleles were not associated with haplotypes of C-318T/A+49G of the CTLA-4 gene in Chinese patients with UC. The longer alleles of the CTLA-4 gene microsatellite polymorphism were strongly associated with UC in Chinese patients. Copyright © 2006 by Lippincott Williams & Wilkins.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.ibdjournal.comen_HK
dc.relation.ispartofInflammatory Bowel Diseasesen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAntigens, CDen_HK
dc.subject.meshAntigens, Differentiation - geneticsen_HK
dc.subject.meshAsian Continental Ancestry Groupen_HK
dc.subject.meshCTLA-4 Antigenen_HK
dc.subject.meshChina - epidemiologyen_HK
dc.subject.meshColitis, Ulcerative - geneticsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGenetic Predisposition to Diseaseen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMicrosatellite Repeats - geneticsen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPolymorphism, Geneticen_HK
dc.titleAssociation of CTLA-4 gene microsatellite polymorphism with ulcerative colitis in Chinese patientsen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, BCY:bcywong@hku.hken_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1097/01.MIB.0000217339.61183.dden_HK
dc.identifier.pmid16670525en_HK
dc.identifier.scopuseid_2-s2.0-33646817094en_HK
dc.identifier.hkuros117718en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646817094&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue5en_HK
dc.identifier.spage369en_HK
dc.identifier.epage373en_HK
dc.identifier.isiWOS:000237370300006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridJiang, Y=13608424700en_HK
dc.identifier.scopusauthoridXia, B=7102762263en_HK
dc.identifier.scopusauthoridJiang, L=36077340600en_HK
dc.identifier.scopusauthoridLv, M=36728902100en_HK
dc.identifier.scopusauthoridGuo, Q=12792639800en_HK
dc.identifier.scopusauthoridChen, M=35080134300en_HK
dc.identifier.scopusauthoridLi, J=36065114100en_HK
dc.identifier.scopusauthoridXia, HHX=8757161400en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK

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