Article: Melatonin reduces infarction volume in a photothrombotic stroke model in the wild-type but not cyclooxygenase-1-gene knockout mice

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TitleMelatonin reduces infarction volume in a photothrombotic stroke model in the wild-type but not cyclooxygenase-1-gene knockout mice
AuthorsLiang, YZ1
Cheung, RTF1
Liu, S2
Li, G1
Huang, L2
Issue Date2006
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPI
CitationJournal Of Pineal Research, 2006, v. 41 n. 2, p. 150-156 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-079X.2006.00349.x
AbstractCyclooxygenase (COX)-2 plays a harmful role in cerebral ischemic/reperfusion injury, but the role of COX-1 is uncertain. In the present study, cerebral infarct was induced by photothrombosis. Intraperitoneal injections of melatonin at 15 g/kg or its vehicle were made at 0.5 hr before stroke and 24 and 48 hr after stroke. Cerebral blood flow (CBF) in the penumbra was monitored during stroke using a laser Doppler flowmeter. Sensorimotor behavior was evaluated using the turning in an alley and falling from a pole tests at 1 hr before stroke and 24 and 48 hr after stroke. Infarct volume was determined from the T2-weighted magnetic resonance images at 72 hr after stroke. During the first 15 min of stroke, CBF decreased in the penumbra in both homozygous COX-1-gene knockout and wild-type mice. Melatonin treatment improved the penumbral CBF in the wild-type mice. Mild poststroke impairment in sensorimotor behavior was detected by the turning in an alley test in which the COX-1-gene knockout mice performed better. Melatonin treatment did not affect the poststroke sensorimotor behavior. The relative infarct volume at 72 hr after stroke was 8.1% and 8.4% in the COX-1-gene knockout and wild-type mice, respectively. Melatonin treatment reduced the relative infarct volume to 6.3% in the latter but not in the former (8.2%). Thus, COX-1-gene knockout does not affect the brain's susceptibility to photothrombotic stroke. Melatonin treatment reduces infarct size in the wild-type mice following photothrombotic stroke partly via maintenance of penumbral CBF in which the COX-1-gene may play a role. © 2006 The Authors.
ISSN0742-3098
2011 Impact Factor: 5.794
2011 SCImago Journal Rankings: 0.244
DOIhttp://dx.doi.org/10.1111/j.1600-079X.2006.00349.x
ISI Accession Number IDWOS:000239371000009
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLiang, YZ
dc.contributor.authorCheung, RTF
dc.contributor.authorLiu, S
dc.contributor.authorLi, G
dc.contributor.authorHuang, L
dc.date.accessioned2010-09-06T07:41:53Z
dc.date.available2010-09-06T07:41:53Z
dc.date.issued2006
dc.description.abstractCyclooxygenase (COX)-2 plays a harmful role in cerebral ischemic/reperfusion injury, but the role of COX-1 is uncertain. In the present study, cerebral infarct was induced by photothrombosis. Intraperitoneal injections of melatonin at 15 g/kg or its vehicle were made at 0.5 hr before stroke and 24 and 48 hr after stroke. Cerebral blood flow (CBF) in the penumbra was monitored during stroke using a laser Doppler flowmeter. Sensorimotor behavior was evaluated using the turning in an alley and falling from a pole tests at 1 hr before stroke and 24 and 48 hr after stroke. Infarct volume was determined from the T2-weighted magnetic resonance images at 72 hr after stroke. During the first 15 min of stroke, CBF decreased in the penumbra in both homozygous COX-1-gene knockout and wild-type mice. Melatonin treatment improved the penumbral CBF in the wild-type mice. Mild poststroke impairment in sensorimotor behavior was detected by the turning in an alley test in which the COX-1-gene knockout mice performed better. Melatonin treatment did not affect the poststroke sensorimotor behavior. The relative infarct volume at 72 hr after stroke was 8.1% and 8.4% in the COX-1-gene knockout and wild-type mice, respectively. Melatonin treatment reduced the relative infarct volume to 6.3% in the latter but not in the former (8.2%). Thus, COX-1-gene knockout does not affect the brain's susceptibility to photothrombotic stroke. Melatonin treatment reduces infarct size in the wild-type mice following photothrombotic stroke partly via maintenance of penumbral CBF in which the COX-1-gene may play a role. © 2006 The Authors.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Pineal Research, 2006, v. 41 n. 2, p. 150-156 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-079X.2006.00349.x
dc.identifier.citeulike781038
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-079X.2006.00349.x
dc.identifier.epage156
dc.identifier.hkuros130496
dc.identifier.isiWOS:000239371000009
dc.identifier.issn0742-3098
2011 Impact Factor: 5.794
2011 SCImago Journal Rankings: 0.244
dc.identifier.issue2
dc.identifier.openurl
dc.identifier.pmid16879321
dc.identifier.scopuseid_2-s2.0-33746508995
dc.identifier.spage150
dc.identifier.urihttp://hdl.handle.net/10722/78349
dc.identifier.volume41
dc.languageeng
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPI
dc.publisher.placeDenmark
dc.relation.ispartofJournal of Pineal Research
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshBrain - blood supply - drug effects - pathology
dc.subject.meshBrain Edema - pathology
dc.subject.meshCerebral Infarction - drug therapy - pathology - physiopathology
dc.subject.meshCerebrovascular Circulation - drug effects
dc.subject.meshCyclooxygenase 1 - genetics
dc.subject.meshDisease Models, Animal
dc.subject.meshLaser-Doppler Flowmetry
dc.subject.meshMelatonin - administration & dosage - therapeutic use
dc.subject.meshMice
dc.subject.meshMice, Knockout
dc.subject.meshMotor Skills
dc.subject.meshNeuroprotective Agents - therapeutic use
dc.subject.meshReperfusion Injury - physiopathology
dc.subject.meshRose Bengal
dc.subject.meshThrombosis - metabolism
dc.titleMelatonin reduces infarction volume in a photothrombotic stroke model in the wild-type but not cyclooxygenase-1-gene knockout mice
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Jinan University