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Article: Peripheral and central administration of neuropeptide Y in a rat middle cerebral artery occlusion stroke model reduces cerebral blood flow and increases infarct volume

TitlePeripheral and central administration of neuropeptide Y in a rat middle cerebral artery occlusion stroke model reduces cerebral blood flow and increases infarct volume
Authors
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 2002, v. 927 n. 2, p. 138-143 How to Cite?
AbstractRecent studies have shown increased immunoreactivity for neuropeptide Y (NPY) within the perilesional cortex following experimental middle cerebral artery occlusion (MCAO) or focal excitotoxic damage. Downregulation of the NPY Y1 receptor gene using an antisense oligodeoxynucleotide produced a doubling of the infarct volume, implying that NPY may mediate neuroprotection against focal ischemia. The effects of treatment with NPY on infarct volume and hemodynamic parameters were investigated in the present study. Adult male Sprague-Dawley rats were anesthetized with sodium pentobarbital to undergo right-sided endovascular MCAO for 2 h. A single dose of NPY was given via intracarotid injection (10 μg/kg) at the beginning of reperfusion, intracisternal injection (10 or 30 μg/kg) at 30 min of ischemia, or intracerebroventricular (i.c.v.) injection (10 or 70 μg/kg) at 30 min of ischemia. Control groups received the vehicle only via the same route. Body temperature was maintained constant, and hemodynamic parameters were monitored during anesthesia. Laser Doppler flowmetry was used to monitor the regional cerebral blood flow (rCBF) during ischemia and reperfusion in some rats. The rats were decapitated on day 3, and their brains were cut into 2-mm thick coronal slices before reaction with a 2% solution of 2,3,5-triphenyltetrazolium chloride to reveal the infarct. Compared to the respective control groups, NPY treatment via any method of administration increased the relative infarct volume. Suppression of rCBF was observed during reperfusion. These results indicate that peripheral or central administration of NPY impairs reperfusion following experimental MCAO and worsens the outcome of focal cerebral ischemia. © 2002 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78341
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, SHen_HK
dc.contributor.authorCheung, RTFen_HK
dc.date.accessioned2010-09-06T07:41:48Z-
dc.date.available2010-09-06T07:41:48Z-
dc.date.issued2002en_HK
dc.identifier.citationBrain Research, 2002, v. 927 n. 2, p. 138-143en_HK
dc.identifier.issn0006-8993en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78341-
dc.description.abstractRecent studies have shown increased immunoreactivity for neuropeptide Y (NPY) within the perilesional cortex following experimental middle cerebral artery occlusion (MCAO) or focal excitotoxic damage. Downregulation of the NPY Y1 receptor gene using an antisense oligodeoxynucleotide produced a doubling of the infarct volume, implying that NPY may mediate neuroprotection against focal ischemia. The effects of treatment with NPY on infarct volume and hemodynamic parameters were investigated in the present study. Adult male Sprague-Dawley rats were anesthetized with sodium pentobarbital to undergo right-sided endovascular MCAO for 2 h. A single dose of NPY was given via intracarotid injection (10 μg/kg) at the beginning of reperfusion, intracisternal injection (10 or 30 μg/kg) at 30 min of ischemia, or intracerebroventricular (i.c.v.) injection (10 or 70 μg/kg) at 30 min of ischemia. Control groups received the vehicle only via the same route. Body temperature was maintained constant, and hemodynamic parameters were monitored during anesthesia. Laser Doppler flowmetry was used to monitor the regional cerebral blood flow (rCBF) during ischemia and reperfusion in some rats. The rats were decapitated on day 3, and their brains were cut into 2-mm thick coronal slices before reaction with a 2% solution of 2,3,5-triphenyltetrazolium chloride to reveal the infarct. Compared to the respective control groups, NPY treatment via any method of administration increased the relative infarct volume. Suppression of rCBF was observed during reperfusion. These results indicate that peripheral or central administration of NPY impairs reperfusion following experimental MCAO and worsens the outcome of focal cerebral ischemia. © 2002 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_HK
dc.relation.ispartofBrain Researchen_HK
dc.rightsBrain Research. Copyright © Elsevier BV.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCerebral Infarction - etiology - pathologyen_HK
dc.subject.meshCerebrovascular Circulation - drug effects - physiologyen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshHemodynamics - drug effectsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInfarction, Middle Cerebral Artery - physiopathology - prevention & controlen_HK
dc.subject.meshInjections, Intra-Arterialen_HK
dc.subject.meshInjections, Intraventricularen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMicroinjectionsen_HK
dc.subject.meshNeuropeptide Y - administration & dosage - pharmacologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.titlePeripheral and central administration of neuropeptide Y in a rat middle cerebral artery occlusion stroke model reduces cerebral blood flow and increases infarct volumeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-8993&volume=927&spage=138&epage=143&date=2002&atitle=Peripheral+and+central+administration+of+neuropeptide+Y+in+a+rat+middle+cerebral+artery+occlusion+stroke+model+reduces+cerebral+blood+flow+and+increases+infarct+volumeen_HK
dc.identifier.emailCheung, RTF:rtcheung@hku.hken_HK
dc.identifier.authorityCheung, RTF=rp00434en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0006-8993(01)03336-4en_HK
dc.identifier.pmid11821007-
dc.identifier.scopuseid_2-s2.0-0037081504en_HK
dc.identifier.hkuros87782en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037081504&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume927en_HK
dc.identifier.issue2en_HK
dc.identifier.spage138en_HK
dc.identifier.epage143en_HK
dc.identifier.isiWOS:000173923000003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, SH=12806098400en_HK
dc.identifier.scopusauthoridCheung, RTF=7202397498en_HK

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