File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Cloning and expression of PDK4, FOXO1A and DYRK1A from the hibernating greater horseshoe bat (Rhinolophus ferrumequinum)

TitleCloning and expression of PDK4, FOXO1A and DYRK1A from the hibernating greater horseshoe bat (Rhinolophus ferrumequinum)
Authors
Issue Date2007
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cbpb
Citation
Comparative Biochemistry And Physiology - B Biochemistry And Molecular Biology, 2007, v. 146 n. 2, p. 166-171 How to Cite?
AbstractPyruvate dehydrogenase kinase isoenzyme 4 (PDK4) cDNA was cloned from the brain of greater horseshoe bat (Rhinolophus ferrumequinum). The deduced amino acid sequence shares strong homology with these PDK4 of other mammals. Moreover, we partially cloned homologues of dual-specificity tyrosine-phosphorylated and regulated protein kinase 1A (DYRK1A), and forkhead box protein O1A (FOXO1A) from greater horseshoe bat. Among five different tissues tested, PDK4 mRNA was highly expressed in the heart, white adipose tissue and muscle, but weakly expressed in the brain and liver, while DYRK1A and FOXO1A were expressed in all five tissues. Moreover, the transcript levels of PDK4, DYRK1A, and FOXO1A were measured in the heart, white adipose tissue and muscle of hibernating and arousal greater horseshoe bats by Northern blot and real time PCR. The results showed that transcript level of PDK4 was significantly higher in white adipose tissue. Expression level of DYRK1A was significantly higher in hibernating state in white adipose tissue, and expression level of FOXO1A was significantly higher in muscle in aroused state. These results suggest that up-regulation of the transcript levels of PDK4 during hibernation were not regulated via DYRK1A and FOXO1A in white adipose tissue and muscle, and the possible presence of another isoenzyme of PDK which is responsible for the tissue-specific regulation of Pyruvate dehydrogenase complex (PDC) activity in the bat heart during hibernation. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78262
ISSN
2015 Impact Factor: 1.651
2015 SCImago Journal Rankings: 0.709
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Jen_HK
dc.contributor.authorSun, Men_HK
dc.contributor.authorLiang, Ben_HK
dc.contributor.authorXu, Aen_HK
dc.contributor.authorZhang, Sen_HK
dc.contributor.authorWu, Den_HK
dc.date.accessioned2010-09-06T07:40:56Z-
dc.date.available2010-09-06T07:40:56Z-
dc.date.issued2007en_HK
dc.identifier.citationComparative Biochemistry And Physiology - B Biochemistry And Molecular Biology, 2007, v. 146 n. 2, p. 166-171en_HK
dc.identifier.issn1096-4959en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78262-
dc.description.abstractPyruvate dehydrogenase kinase isoenzyme 4 (PDK4) cDNA was cloned from the brain of greater horseshoe bat (Rhinolophus ferrumequinum). The deduced amino acid sequence shares strong homology with these PDK4 of other mammals. Moreover, we partially cloned homologues of dual-specificity tyrosine-phosphorylated and regulated protein kinase 1A (DYRK1A), and forkhead box protein O1A (FOXO1A) from greater horseshoe bat. Among five different tissues tested, PDK4 mRNA was highly expressed in the heart, white adipose tissue and muscle, but weakly expressed in the brain and liver, while DYRK1A and FOXO1A were expressed in all five tissues. Moreover, the transcript levels of PDK4, DYRK1A, and FOXO1A were measured in the heart, white adipose tissue and muscle of hibernating and arousal greater horseshoe bats by Northern blot and real time PCR. The results showed that transcript level of PDK4 was significantly higher in white adipose tissue. Expression level of DYRK1A was significantly higher in hibernating state in white adipose tissue, and expression level of FOXO1A was significantly higher in muscle in aroused state. These results suggest that up-regulation of the transcript levels of PDK4 during hibernation were not regulated via DYRK1A and FOXO1A in white adipose tissue and muscle, and the possible presence of another isoenzyme of PDK which is responsible for the tissue-specific regulation of Pyruvate dehydrogenase complex (PDC) activity in the bat heart during hibernation. © 2006 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cbpben_HK
dc.relation.ispartofComparative Biochemistry and Physiology - B Biochemistry and Molecular Biologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBlotting, Northernen_HK
dc.subject.meshChiroptera - genetics - physiologyen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshDNA, Complementary - chemistry - genetics - isolation & purificationen_HK
dc.subject.meshForkhead Transcription Factors - geneticsen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshHibernationen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshProtein Kinases - geneticsen_HK
dc.subject.meshProtein-Serine-Threonine Kinases - geneticsen_HK
dc.subject.meshProtein-Tyrosine Kinases - geneticsen_HK
dc.subject.meshRNA, Messenger - genetics - metabolismen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.titleCloning and expression of PDK4, FOXO1A and DYRK1A from the hibernating greater horseshoe bat (Rhinolophus ferrumequinum)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1096-4959&volume=142&spage=166&epage=71&date=2007&atitle=Cloning+and+expression+of+PDK4,+FOXO1A+and+DYRK1A+from+the+hibernating+greater+horseshoe+bat+(Rhinolophus+ferrumequinum).en_HK
dc.identifier.emailXu, A:amxu@hkucc.hku.hken_HK
dc.identifier.authorityXu, A=rp00485en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.cbpb.2006.10.095en_HK
dc.identifier.pmid17140834en_HK
dc.identifier.scopuseid_2-s2.0-33846837221en_HK
dc.identifier.hkuros129067en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846837221&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume146en_HK
dc.identifier.issue2en_HK
dc.identifier.spage166en_HK
dc.identifier.epage171en_HK
dc.identifier.isiWOS:000244675900003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChen, J=9238628400en_HK
dc.identifier.scopusauthoridSun, M=36139760900en_HK
dc.identifier.scopusauthoridLiang, B=55165675800en_HK
dc.identifier.scopusauthoridXu, A=7202655409en_HK
dc.identifier.scopusauthoridZhang, S=35212214400en_HK
dc.identifier.scopusauthoridWu, D=7404297751en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats