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Article: Properties of ion channels in rabbit mesenchymal stem cells from bone marrow

TitleProperties of ion channels in rabbit mesenchymal stem cells from bone marrow
Authors
KeywordsCalcium-activated potassium current
Delayed rectifier potassium current
Inward rectifier potassium current
Ion channels
Mesenchymal stem cells
Rabbit
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2006, v. 348 n. 1, p. 301-309 How to Cite?
AbstractThe present study was designed to investigate properties of ion channels in undifferentiated rabbit mesenchymal stem cells (MSCs) from bone marrow using whole-cell patch-clamp and RT-PCR techniques. It was found that three types of outward currents were present in rabbit MSCs, including an inward rectifier K+ current (IKir), a noise-like Ca2+-activated K+ current (IKCa) co-present with delayed rectifier K+ current (IKDR). IKir was inhibited by Ba2+, while IKCa was inhibited by paxilline (a blocker of big conductance IKCa channels) and clotrimazole (an inhibitor of intermediate conductance IKCa channels). IKDR exhibited a slow inactivation, "U-shaped" voltage-dependent inactivation, and slow recovery from inactivation, and the current was inhibited by tetraethylammonium or 4-aminopyridine. RT-PCR revealed the molecular identities for the functional ionic currents, including Kir1.1 (possibly responsible for IKir), KCa1.1 and KCa3.1 (possibly responsible for IKCa), and Kv1.2, Kv2.1, and Kv2.2 (possibly responsible for IKDR). These results demonstrate for the first time that three types of functional ion channel currents (i.e., IKir, IKCa, and IKDR) are present in rabbit MSCs from bone marrow. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78241
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDeng, XLen_HK
dc.contributor.authorSun, HYen_HK
dc.contributor.authorLau, CPen_HK
dc.contributor.authorLi, GRen_HK
dc.date.accessioned2010-09-06T07:40:42Z-
dc.date.available2010-09-06T07:40:42Z-
dc.date.issued2006en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2006, v. 348 n. 1, p. 301-309en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/78241-
dc.description.abstractThe present study was designed to investigate properties of ion channels in undifferentiated rabbit mesenchymal stem cells (MSCs) from bone marrow using whole-cell patch-clamp and RT-PCR techniques. It was found that three types of outward currents were present in rabbit MSCs, including an inward rectifier K+ current (IKir), a noise-like Ca2+-activated K+ current (IKCa) co-present with delayed rectifier K+ current (IKDR). IKir was inhibited by Ba2+, while IKCa was inhibited by paxilline (a blocker of big conductance IKCa channels) and clotrimazole (an inhibitor of intermediate conductance IKCa channels). IKDR exhibited a slow inactivation, "U-shaped" voltage-dependent inactivation, and slow recovery from inactivation, and the current was inhibited by tetraethylammonium or 4-aminopyridine. RT-PCR revealed the molecular identities for the functional ionic currents, including Kir1.1 (possibly responsible for IKir), KCa1.1 and KCa3.1 (possibly responsible for IKCa), and Kv1.2, Kv2.1, and Kv2.2 (possibly responsible for IKDR). These results demonstrate for the first time that three types of functional ion channel currents (i.e., IKir, IKCa, and IKDR) are present in rabbit MSCs from bone marrow. © 2006 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectCalcium-activated potassium currenten_HK
dc.subjectDelayed rectifier potassium currenten_HK
dc.subjectInward rectifier potassium currenten_HK
dc.subjectIon channelsen_HK
dc.subjectMesenchymal stem cellsen_HK
dc.subjectRabbiten_HK
dc.subject.meshBone Marrow Cells - physiology-
dc.subject.meshMesenchymal Stem Cells - physiology-
dc.subject.meshPatch-Clamp Techniques-
dc.subject.meshPotassium Channels - analysis - physiology-
dc.subject.meshSpecies Specificity-
dc.titleProperties of ion channels in rabbit mesenchymal stem cells from bone marrowen_HK
dc.typeArticleen_HK
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2006.07.054en_HK
dc.identifier.pmid16876113en_HK
dc.identifier.scopuseid_2-s2.0-33746852408en_HK
dc.identifier.hkuros122218en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33746852408&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume348en_HK
dc.identifier.issue1en_HK
dc.identifier.spage301en_HK
dc.identifier.epage309en_HK
dc.identifier.isiWOS:000239796100042-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDeng, XL=14057894600en_HK
dc.identifier.scopusauthoridSun, HY=35723049200en_HK
dc.identifier.scopusauthoridLau, CP=7401968501en_HK
dc.identifier.scopusauthoridLi, GR=7408462932en_HK

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