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Article: Lymphocyte apoptosis and macrophage function: Correlation with disease activity in systemic lupus erythematosus
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TitleLymphocyte apoptosis and macrophage function: Correlation with disease activity in systemic lupus erythematosus
 
AuthorsJin, O3
Sun, LY3
Zhou, KX2
Zhang, XS3
Feng, XB3
Mok, MY1
Lau, CS1
 
Issue Date2005
 
PublisherSpringer-Verlag London Ltd. The Journal's web site is located at http://link.springer.de/link/service/journals/10067/
 
CitationClinical Rheumatology, 2005, v. 24 n. 2, p. 107-110 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10067-004-0972-x
 
AbstractIncreased lymphocyte apoptosis and defects in macrophage removal of apoptotic cells have been suggested to contribute to the development of systemic lupus erythematosus (SLE). The aim of this study was to investigate the relationship between peripheral lymphocyte apoptosis, macrophage function as determined by the serum levels of neopterin and interferon-γ (IFN-γ), and SLE disease activity. Peripheral apoptotic lymphocytes (AL) were detected by annexin V-fluorescein isothiocyanate (FITC) staining and flow cytometry. Serum levels of neopterin and IFN-γ were measured by enzyme-linked immunosorbent assay (ELISA). SLE disease activity was determined using the systemic lupus activity measure (SLAM) and the serum titer of anti-dsDNA antibodies. The percentage of AL in the peripheral blood of active SLE patients was significantly higher (13.07 ± 7.39%, n = 30) than that of the inactive SLE patients (4.08 ± 3.55%, n = 8, p < 0.01) and normal controls (5.13 ± 3.37%, n = 11, p < 0.01). Serum levels of neopterin in active SLE patients were significantly higher (1.39 ± 1.10 μg/dl, n = 22) than in controls (0.26 ± 0.19 μg/dl, n = 20, p < 0.01). Serum levels of IFN-γ in active SLE patients were elevated (58.97 ± 34.52 ng/l, n = 15) when compared with controls (28.06 ± 2.35 ng/l, n = 16, p < 0.05). The percentage of AL correlated significantly with serum levels of neopterin (r = 0.446, p < 0.05, n = 22) and SLAM score (r = 0.533, p < 0.001, n = 38), but not with the serum levels of IFN-γ. The SLAM score also correlated with the serum levels of neopterin (r = 0.485, p < 0.05, n = 22), but not with those of IFN-γ. Our study supported the hypothesis that increased lymphocyte apoptosis has a pathogenic role in SLE. The increased levels of serum neopterin may suggest an attempt of the patients' macrophage system to remove the apoptotic cell excess. Since serum levels of neopterin correlated with the overall lupus disease activity, they may be regarded as an index of SLE disease activity. © Clinical Rheumatology 2004.
 
ISSN0770-3198
2013 Impact Factor: 1.774
2013 SCImago Journal Rankings: 0.862
 
DOIhttp://dx.doi.org/10.1007/s10067-004-0972-x
 
ISI Accession Number IDWOS:000228256200005
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorJin, O
 
dc.contributor.authorSun, LY
 
dc.contributor.authorZhou, KX
 
dc.contributor.authorZhang, XS
 
dc.contributor.authorFeng, XB
 
dc.contributor.authorMok, MY
 
dc.contributor.authorLau, CS
 
dc.date.accessioned2010-09-06T07:39:47Z
 
dc.date.available2010-09-06T07:39:47Z
 
dc.date.issued2005
 
dc.description.abstractIncreased lymphocyte apoptosis and defects in macrophage removal of apoptotic cells have been suggested to contribute to the development of systemic lupus erythematosus (SLE). The aim of this study was to investigate the relationship between peripheral lymphocyte apoptosis, macrophage function as determined by the serum levels of neopterin and interferon-γ (IFN-γ), and SLE disease activity. Peripheral apoptotic lymphocytes (AL) were detected by annexin V-fluorescein isothiocyanate (FITC) staining and flow cytometry. Serum levels of neopterin and IFN-γ were measured by enzyme-linked immunosorbent assay (ELISA). SLE disease activity was determined using the systemic lupus activity measure (SLAM) and the serum titer of anti-dsDNA antibodies. The percentage of AL in the peripheral blood of active SLE patients was significantly higher (13.07 ± 7.39%, n = 30) than that of the inactive SLE patients (4.08 ± 3.55%, n = 8, p < 0.01) and normal controls (5.13 ± 3.37%, n = 11, p < 0.01). Serum levels of neopterin in active SLE patients were significantly higher (1.39 ± 1.10 μg/dl, n = 22) than in controls (0.26 ± 0.19 μg/dl, n = 20, p < 0.01). Serum levels of IFN-γ in active SLE patients were elevated (58.97 ± 34.52 ng/l, n = 15) when compared with controls (28.06 ± 2.35 ng/l, n = 16, p < 0.05). The percentage of AL correlated significantly with serum levels of neopterin (r = 0.446, p < 0.05, n = 22) and SLAM score (r = 0.533, p < 0.001, n = 38), but not with the serum levels of IFN-γ. The SLAM score also correlated with the serum levels of neopterin (r = 0.485, p < 0.05, n = 22), but not with those of IFN-γ. Our study supported the hypothesis that increased lymphocyte apoptosis has a pathogenic role in SLE. The increased levels of serum neopterin may suggest an attempt of the patients' macrophage system to remove the apoptotic cell excess. Since serum levels of neopterin correlated with the overall lupus disease activity, they may be regarded as an index of SLE disease activity. © Clinical Rheumatology 2004.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationClinical Rheumatology, 2005, v. 24 n. 2, p. 107-110 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10067-004-0972-x
 
dc.identifier.doihttp://dx.doi.org/10.1007/s10067-004-0972-x
 
dc.identifier.epage110
 
dc.identifier.hkuros100576
 
dc.identifier.isiWOS:000228256200005
 
dc.identifier.issn0770-3198
2013 Impact Factor: 1.774
2013 SCImago Journal Rankings: 0.862
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmid15818511
 
dc.identifier.scopuseid_2-s2.0-17544364539
 
dc.identifier.spage107
 
dc.identifier.urihttp://hdl.handle.net/10722/78155
 
dc.identifier.volume24
 
dc.languageeng
 
dc.publisherSpringer-Verlag London Ltd. The Journal's web site is located at http://link.springer.de/link/service/journals/10067/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofClinical Rheumatology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdolescent
 
dc.subject.meshAdult
 
dc.subject.meshApoptosis - immunology
 
dc.subject.meshFemale
 
dc.subject.meshHumans
 
dc.subject.meshInterferon-gamma - blood
 
dc.subject.meshLupus Erythematosus, Systemic - blood - immunology
 
dc.subject.meshLymphocytes - cytology - immunology
 
dc.subject.meshMacrophages - immunology
 
dc.subject.meshMale
 
dc.subject.meshMiddle Aged
 
dc.subject.meshNeopterin - blood
 
dc.subject.meshSeverity of Illness Index
 
dc.titleLymphocyte apoptosis and macrophage function: Correlation with disease activity in systemic lupus erythematosus
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Nanjing General Hospital of Nanjing Command
  3. The Affiliated DrumTower Hospital of Nanjing University Medical School