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Article: Lymphocyte apoptosis and macrophage function: Correlation with disease activity in systemic lupus erythematosus

TitleLymphocyte apoptosis and macrophage function: Correlation with disease activity in systemic lupus erythematosus
Authors
Issue Date2005
PublisherSpringer-Verlag London Ltd. The Journal's web site is located at http://link.springer.de/link/service/journals/10067/
Citation
Clinical Rheumatology, 2005, v. 24 n. 2, p. 107-110 How to Cite?
AbstractIncreased lymphocyte apoptosis and defects in macrophage removal of apoptotic cells have been suggested to contribute to the development of systemic lupus erythematosus (SLE). The aim of this study was to investigate the relationship between peripheral lymphocyte apoptosis, macrophage function as determined by the serum levels of neopterin and interferon-γ (IFN-γ), and SLE disease activity. Peripheral apoptotic lymphocytes (AL) were detected by annexin V-fluorescein isothiocyanate (FITC) staining and flow cytometry. Serum levels of neopterin and IFN-γ were measured by enzyme-linked immunosorbent assay (ELISA). SLE disease activity was determined using the systemic lupus activity measure (SLAM) and the serum titer of anti-dsDNA antibodies. The percentage of AL in the peripheral blood of active SLE patients was significantly higher (13.07 ± 7.39%, n = 30) than that of the inactive SLE patients (4.08 ± 3.55%, n = 8, p < 0.01) and normal controls (5.13 ± 3.37%, n = 11, p < 0.01). Serum levels of neopterin in active SLE patients were significantly higher (1.39 ± 1.10 μg/dl, n = 22) than in controls (0.26 ± 0.19 μg/dl, n = 20, p < 0.01). Serum levels of IFN-γ in active SLE patients were elevated (58.97 ± 34.52 ng/l, n = 15) when compared with controls (28.06 ± 2.35 ng/l, n = 16, p < 0.05). The percentage of AL correlated significantly with serum levels of neopterin (r = 0.446, p < 0.05, n = 22) and SLAM score (r = 0.533, p < 0.001, n = 38), but not with the serum levels of IFN-γ. The SLAM score also correlated with the serum levels of neopterin (r = 0.485, p < 0.05, n = 22), but not with those of IFN-γ. Our study supported the hypothesis that increased lymphocyte apoptosis has a pathogenic role in SLE. The increased levels of serum neopterin may suggest an attempt of the patients' macrophage system to remove the apoptotic cell excess. Since serum levels of neopterin correlated with the overall lupus disease activity, they may be regarded as an index of SLE disease activity. © Clinical Rheumatology 2004.
Persistent Identifierhttp://hdl.handle.net/10722/78155
ISSN
2014 Impact Factor: 1.696
2014 SCImago Journal Rankings: 0.588
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, Oen_HK
dc.contributor.authorSun, LYen_HK
dc.contributor.authorZhou, KXen_HK
dc.contributor.authorZhang, XSen_HK
dc.contributor.authorFeng, XBen_HK
dc.contributor.authorMok, MYen_HK
dc.contributor.authorLau, CSen_HK
dc.date.accessioned2010-09-06T07:39:47Z-
dc.date.available2010-09-06T07:39:47Z-
dc.date.issued2005en_HK
dc.identifier.citationClinical Rheumatology, 2005, v. 24 n. 2, p. 107-110en_HK
dc.identifier.issn0770-3198en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78155-
dc.description.abstractIncreased lymphocyte apoptosis and defects in macrophage removal of apoptotic cells have been suggested to contribute to the development of systemic lupus erythematosus (SLE). The aim of this study was to investigate the relationship between peripheral lymphocyte apoptosis, macrophage function as determined by the serum levels of neopterin and interferon-γ (IFN-γ), and SLE disease activity. Peripheral apoptotic lymphocytes (AL) were detected by annexin V-fluorescein isothiocyanate (FITC) staining and flow cytometry. Serum levels of neopterin and IFN-γ were measured by enzyme-linked immunosorbent assay (ELISA). SLE disease activity was determined using the systemic lupus activity measure (SLAM) and the serum titer of anti-dsDNA antibodies. The percentage of AL in the peripheral blood of active SLE patients was significantly higher (13.07 ± 7.39%, n = 30) than that of the inactive SLE patients (4.08 ± 3.55%, n = 8, p < 0.01) and normal controls (5.13 ± 3.37%, n = 11, p < 0.01). Serum levels of neopterin in active SLE patients were significantly higher (1.39 ± 1.10 μg/dl, n = 22) than in controls (0.26 ± 0.19 μg/dl, n = 20, p < 0.01). Serum levels of IFN-γ in active SLE patients were elevated (58.97 ± 34.52 ng/l, n = 15) when compared with controls (28.06 ± 2.35 ng/l, n = 16, p < 0.05). The percentage of AL correlated significantly with serum levels of neopterin (r = 0.446, p < 0.05, n = 22) and SLAM score (r = 0.533, p < 0.001, n = 38), but not with the serum levels of IFN-γ. The SLAM score also correlated with the serum levels of neopterin (r = 0.485, p < 0.05, n = 22), but not with those of IFN-γ. Our study supported the hypothesis that increased lymphocyte apoptosis has a pathogenic role in SLE. The increased levels of serum neopterin may suggest an attempt of the patients' macrophage system to remove the apoptotic cell excess. Since serum levels of neopterin correlated with the overall lupus disease activity, they may be regarded as an index of SLE disease activity. © Clinical Rheumatology 2004.en_HK
dc.languageengen_HK
dc.publisherSpringer-Verlag London Ltd. The Journal's web site is located at http://link.springer.de/link/service/journals/10067/en_HK
dc.relation.ispartofClinical Rheumatologyen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshApoptosis - immunologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInterferon-gamma - blooden_HK
dc.subject.meshLupus Erythematosus, Systemic - blood - immunologyen_HK
dc.subject.meshLymphocytes - cytology - immunologyen_HK
dc.subject.meshMacrophages - immunologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshNeopterin - blooden_HK
dc.subject.meshSeverity of Illness Indexen_HK
dc.titleLymphocyte apoptosis and macrophage function: Correlation with disease activity in systemic lupus erythematosusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0770-3198&volume=24&spage=107&epage=110&date=2005&atitle=Lymphocyte+apoptosis+and+macrophage+function:+correlation+with+disease+activity+in+systemic+lupus+erythematosusen_HK
dc.identifier.emailMok, MY:temy@hkucc.hku.hken_HK
dc.identifier.emailLau, CS:cslau@hku.hken_HK
dc.identifier.authorityMok, MY=rp00490en_HK
dc.identifier.authorityLau, CS=rp01348en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10067-004-0972-xen_HK
dc.identifier.pmid15818511en_HK
dc.identifier.scopuseid_2-s2.0-17544364539en_HK
dc.identifier.hkuros100576en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-17544364539&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue2en_HK
dc.identifier.spage107en_HK
dc.identifier.epage110en_HK
dc.identifier.isiWOS:000228256200005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridJin, O=7004432895en_HK
dc.identifier.scopusauthoridSun, LY=35265069800en_HK
dc.identifier.scopusauthoridZhou, KX=8218830200en_HK
dc.identifier.scopusauthoridZhang, XS=36065960100en_HK
dc.identifier.scopusauthoridFeng, XB=7403047175en_HK
dc.identifier.scopusauthoridMok, MY=7006024184en_HK
dc.identifier.scopusauthoridLau, CS=14035682100en_HK

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