Article: Lymphocyte apoptosis and macrophage function: Correlation with disease activity in systemic lupus erythematosus

File Download Links for fulltext
(May Require Subscription)
Supplementary

  • Basic View
  • Metadata View
  • XML View
TitleLymphocyte apoptosis and macrophage function: Correlation with disease activity in systemic lupus erythematosus
AuthorsJin, O3
Sun, LY3
Zhou, KX2
Zhang, XS3
Feng, XB3
Mok, MY1
Lau, CS1
Issue Date2005
PublisherSpringer-Verlag London Ltd. The Journal's web site is located at http://link.springer.de/link/service/journals/10067/
CitationClinical Rheumatology, 2005, v. 24 n. 2, p. 107-110 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10067-004-0972-x
AbstractIncreased lymphocyte apoptosis and defects in macrophage removal of apoptotic cells have been suggested to contribute to the development of systemic lupus erythematosus (SLE). The aim of this study was to investigate the relationship between peripheral lymphocyte apoptosis, macrophage function as determined by the serum levels of neopterin and interferon-γ (IFN-γ), and SLE disease activity. Peripheral apoptotic lymphocytes (AL) were detected by annexin V-fluorescein isothiocyanate (FITC) staining and flow cytometry. Serum levels of neopterin and IFN-γ were measured by enzyme-linked immunosorbent assay (ELISA). SLE disease activity was determined using the systemic lupus activity measure (SLAM) and the serum titer of anti-dsDNA antibodies. The percentage of AL in the peripheral blood of active SLE patients was significantly higher (13.07 ± 7.39%, n = 30) than that of the inactive SLE patients (4.08 ± 3.55%, n = 8, p < 0.01) and normal controls (5.13 ± 3.37%, n = 11, p < 0.01). Serum levels of neopterin in active SLE patients were significantly higher (1.39 ± 1.10 μg/dl, n = 22) than in controls (0.26 ± 0.19 μg/dl, n = 20, p < 0.01). Serum levels of IFN-γ in active SLE patients were elevated (58.97 ± 34.52 ng/l, n = 15) when compared with controls (28.06 ± 2.35 ng/l, n = 16, p < 0.05). The percentage of AL correlated significantly with serum levels of neopterin (r = 0.446, p < 0.05, n = 22) and SLAM score (r = 0.533, p < 0.001, n = 38), but not with the serum levels of IFN-γ. The SLAM score also correlated with the serum levels of neopterin (r = 0.485, p < 0.05, n = 22), but not with those of IFN-γ. Our study supported the hypothesis that increased lymphocyte apoptosis has a pathogenic role in SLE. The increased levels of serum neopterin may suggest an attempt of the patients' macrophage system to remove the apoptotic cell excess. Since serum levels of neopterin correlated with the overall lupus disease activity, they may be regarded as an index of SLE disease activity. © Clinical Rheumatology 2004.
ISSN0770-3198
2011 Impact Factor: 1.996
2011 SCImago Journal Rankings: 0.143
DOIhttp://dx.doi.org/10.1007/s10067-004-0972-x
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorJin, O
dc.contributor.authorSun, LY
dc.contributor.authorZhou, KX
dc.contributor.authorZhang, XS
dc.contributor.authorFeng, XB
dc.contributor.authorMok, MY
dc.contributor.authorLau, CS
dc.date.accessioned2010-09-06T07:39:47Z
dc.date.available2010-09-06T07:39:47Z
dc.date.issued2005
dc.description.abstractIncreased lymphocyte apoptosis and defects in macrophage removal of apoptotic cells have been suggested to contribute to the development of systemic lupus erythematosus (SLE). The aim of this study was to investigate the relationship between peripheral lymphocyte apoptosis, macrophage function as determined by the serum levels of neopterin and interferon-γ (IFN-γ), and SLE disease activity. Peripheral apoptotic lymphocytes (AL) were detected by annexin V-fluorescein isothiocyanate (FITC) staining and flow cytometry. Serum levels of neopterin and IFN-γ were measured by enzyme-linked immunosorbent assay (ELISA). SLE disease activity was determined using the systemic lupus activity measure (SLAM) and the serum titer of anti-dsDNA antibodies. The percentage of AL in the peripheral blood of active SLE patients was significantly higher (13.07 ± 7.39%, n = 30) than that of the inactive SLE patients (4.08 ± 3.55%, n = 8, p < 0.01) and normal controls (5.13 ± 3.37%, n = 11, p < 0.01). Serum levels of neopterin in active SLE patients were significantly higher (1.39 ± 1.10 μg/dl, n = 22) than in controls (0.26 ± 0.19 μg/dl, n = 20, p < 0.01). Serum levels of IFN-γ in active SLE patients were elevated (58.97 ± 34.52 ng/l, n = 15) when compared with controls (28.06 ± 2.35 ng/l, n = 16, p < 0.05). The percentage of AL correlated significantly with serum levels of neopterin (r = 0.446, p < 0.05, n = 22) and SLAM score (r = 0.533, p < 0.001, n = 38), but not with the serum levels of IFN-γ. The SLAM score also correlated with the serum levels of neopterin (r = 0.485, p < 0.05, n = 22), but not with those of IFN-γ. Our study supported the hypothesis that increased lymphocyte apoptosis has a pathogenic role in SLE. The increased levels of serum neopterin may suggest an attempt of the patients' macrophage system to remove the apoptotic cell excess. Since serum levels of neopterin correlated with the overall lupus disease activity, they may be regarded as an index of SLE disease activity. © Clinical Rheumatology 2004.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationClinical Rheumatology, 2005, v. 24 n. 2, p. 107-110 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10067-004-0972-x
dc.identifier.doihttp://dx.doi.org/10.1007/s10067-004-0972-x
dc.identifier.epage110
dc.identifier.hkuros100576
dc.identifier.isiWOS:000228256200005
dc.identifier.issn0770-3198
2011 Impact Factor: 1.996
2011 SCImago Journal Rankings: 0.143
dc.identifier.issue2
dc.identifier.openurl
dc.identifier.pmid15818511
dc.identifier.scopuseid_2-s2.0-17544364539
dc.identifier.spage107
dc.identifier.urihttp://hdl.handle.net/10722/78155
dc.identifier.volume24
dc.languageeng
dc.publisherSpringer-Verlag London Ltd. The Journal's web site is located at http://link.springer.de/link/service/journals/10067/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofClinical Rheumatology
dc.relation.referencesReferences in Scopus
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshApoptosis - immunology
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshInterferon-gamma - blood
dc.subject.meshLupus Erythematosus, Systemic - blood - immunology
dc.subject.meshLymphocytes - cytology - immunology
dc.subject.meshMacrophages - immunology
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshNeopterin - blood
dc.subject.meshSeverity of Illness Index
dc.titleLymphocyte apoptosis and macrophage function: Correlation with disease activity in systemic lupus erythematosus
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Nanjing General Hospital of Nanjing Command
  3. The Affiliated DrumTower Hospital of Nanjing University Medical School