Article: Characterization of calcium signaling pathways in human preadipocytes

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TitleCharacterization of calcium signaling pathways in human preadipocytes
AuthorsHu, R2 3
He, ML2
Hu, H2 3
Yuan, BX3
Zang, WJ3
Lau, CP2
Tse, HF2
Li, GR1 2
Issue Date2009
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
CitationJournal Of Cellular Physiology, 2009, v. 220 n. 3, p. 765-770 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jcp.21823
AbstractIntracellular free Ca 2+ (Ca i 2+) is an important regulator of many cellular activities; however, Ca 2+ signaling is not well studied in human preadipocytes. The purpose of the present study was to characterize Ca 2+ signal pathways using a confocal scanning technique and RT-PCR. It was found that spontaneous Ca i 2+ oscillations were observed in 12.1% preadipocytes, and number of cells withCa 2+ oscillations was increased to 47.9% by 1% fetal bovine serum. Ca i 2+ oscillations were dependent on Ca 2+ entry mainly via stored-operated Ca 2+ (SOC) entry. They were suppressed by the SOC entry channel blocker La 3+, the phospholipase C (PLC) inhibitor U73122, the inositol trisphosphate receptor (IP3R) blocker 2-amino-ethoxydiphenyl borate, or the sarcoplasmic/endoplasmic reticulum Ca 2+ pump (SERCA) inhibitors thapsigargin and cyclopiazonic acid, but not by ryanodine. The IP3R activator thimerosal increased Ca i 2+ oscillations. In addition, the plasma membrane Ca 2+ pump (PMCA) inhibitor carboxyeosin and Na +-Ca 2+ exchanger (NCX) inhibitor Ni 2+ both suppressed Ca 2+ oscillations. RT-PCR revealed that the mRNAs for IP3R1-3, SERCA1,2, NCX3 and PMCA1,3,4, CaV1.2, and TRPC1,4,6, STIM1 and Orai1 (for SOC entry channels) were significant in human preadipocytes. The present study demonstrates that multiple Ca 2+ signal pathways are present in human preadipocytes, and provides a basis for investigating how Ca 2+ signals regulate biological and physiological activities of human preadipocytes. © 2009 Wiley-Liss, Inc.
ISSN0021-9541
2011 Impact Factor: 3.874
2011 SCImago Journal Rankings: 0.382
DOIhttp://dx.doi.org/10.1002/jcp.21823
ISI Accession Number IDWOS:000268651500027
Funding AgencyGrant Number
General Research Fund770108M
Research Grant Council of Hong Kong and a CRCG of the University of Hong Kong200811159173
Funding Information:

The present study was supported in part by a General Research Fund (HKU 770108M) from Research Grant Council of Hong Kong and a CRCG seeding grant (200811159173) of the University of Hong Kong.

ReferencesReferences in Scopus
GrantsCalcium Signaling Pathways in Human Preadipocytes
DC Field
Value
dc.contributor.authorHu, R
dc.contributor.authorHe, ML
dc.contributor.authorHu, H
dc.contributor.authorYuan, BX
dc.contributor.authorZang, WJ
dc.contributor.authorLau, CP
dc.contributor.authorTse, HF
dc.contributor.authorLi, GR
dc.date.accessioned2010-09-06T07:38:53Z
dc.date.available2010-09-06T07:38:53Z
dc.date.issued2009
dc.description.abstractIntracellular free Ca 2+ (Ca i 2+) is an important regulator of many cellular activities; however, Ca 2+ signaling is not well studied in human preadipocytes. The purpose of the present study was to characterize Ca 2+ signal pathways using a confocal scanning technique and RT-PCR. It was found that spontaneous Ca i 2+ oscillations were observed in 12.1% preadipocytes, and number of cells withCa 2+ oscillations was increased to 47.9% by 1% fetal bovine serum. Ca i 2+ oscillations were dependent on Ca 2+ entry mainly via stored-operated Ca 2+ (SOC) entry. They were suppressed by the SOC entry channel blocker La 3+, the phospholipase C (PLC) inhibitor U73122, the inositol trisphosphate receptor (IP3R) blocker 2-amino-ethoxydiphenyl borate, or the sarcoplasmic/endoplasmic reticulum Ca 2+ pump (SERCA) inhibitors thapsigargin and cyclopiazonic acid, but not by ryanodine. The IP3R activator thimerosal increased Ca i 2+ oscillations. In addition, the plasma membrane Ca 2+ pump (PMCA) inhibitor carboxyeosin and Na +-Ca 2+ exchanger (NCX) inhibitor Ni 2+ both suppressed Ca 2+ oscillations. RT-PCR revealed that the mRNAs for IP3R1-3, SERCA1,2, NCX3 and PMCA1,3,4, CaV1.2, and TRPC1,4,6, STIM1 and Orai1 (for SOC entry channels) were significant in human preadipocytes. The present study demonstrates that multiple Ca 2+ signal pathways are present in human preadipocytes, and provides a basis for investigating how Ca 2+ signals regulate biological and physiological activities of human preadipocytes. © 2009 Wiley-Liss, Inc.
dc.description.grantCalcium Signaling Pathways in Human Preadipocytes
dc.description.grantcode99743
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Cellular Physiology, 2009, v. 220 n. 3, p. 765-770 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jcp.21823
dc.identifier.doihttp://dx.doi.org/10.1002/jcp.21823
dc.identifier.epage770
dc.identifier.hkuros157601
dc.identifier.isiWOS:000268651500027
Funding AgencyGrant Number
General Research Fund770108M
Research Grant Council of Hong Kong and a CRCG of the University of Hong Kong200811159173
Funding Information:

The present study was supported in part by a General Research Fund (HKU 770108M) from Research Grant Council of Hong Kong and a CRCG seeding grant (200811159173) of the University of Hong Kong.

dc.identifier.issn0021-9541
2011 Impact Factor: 3.874
2011 SCImago Journal Rankings: 0.382
dc.identifier.issue3
dc.identifier.openurl
dc.identifier.scopuseid_2-s2.0-67650354016
dc.identifier.spage765
dc.identifier.urihttp://hdl.handle.net/10722/78075
dc.identifier.volume220
dc.languageeng
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Cellular Physiology
dc.relation.referencesReferences in Scopus
dc.rightsJournal of Cellular Physiology. Copyright © John Wiley & Sons, Inc.
dc.titleCharacterization of calcium signaling pathways in human preadipocytes
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong
  3. Xi'an Jiaotong University