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Article: Characterization of calcium signaling pathways in human preadipocytes
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TitleCharacterization of calcium signaling pathways in human preadipocytes
 
AuthorsHu, R2 3
He, ML2
Hu, H2 3
Yuan, BX3
Zang, WJ3
Lau, CP2
Tse, HF2
Li, GR2 1
 
Issue Date2009
 
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
 
CitationJournal Of Cellular Physiology, 2009, v. 220 n. 3, p. 765-770 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jcp.21823
 
AbstractIntracellular free Ca 2+ (Ca i 2+) is an important regulator of many cellular activities; however, Ca 2+ signaling is not well studied in human preadipocytes. The purpose of the present study was to characterize Ca 2+ signal pathways using a confocal scanning technique and RT-PCR. It was found that spontaneous Ca i 2+ oscillations were observed in 12.1% preadipocytes, and number of cells withCa 2+ oscillations was increased to 47.9% by 1% fetal bovine serum. Ca i 2+ oscillations were dependent on Ca 2+ entry mainly via stored-operated Ca 2+ (SOC) entry. They were suppressed by the SOC entry channel blocker La 3+, the phospholipase C (PLC) inhibitor U73122, the inositol trisphosphate receptor (IP3R) blocker 2-amino-ethoxydiphenyl borate, or the sarcoplasmic/endoplasmic reticulum Ca 2+ pump (SERCA) inhibitors thapsigargin and cyclopiazonic acid, but not by ryanodine. The IP3R activator thimerosal increased Ca i 2+ oscillations. In addition, the plasma membrane Ca 2+ pump (PMCA) inhibitor carboxyeosin and Na +-Ca 2+ exchanger (NCX) inhibitor Ni 2+ both suppressed Ca 2+ oscillations. RT-PCR revealed that the mRNAs for IP3R1-3, SERCA1,2, NCX3 and PMCA1,3,4, CaV1.2, and TRPC1,4,6, STIM1 and Orai1 (for SOC entry channels) were significant in human preadipocytes. The present study demonstrates that multiple Ca 2+ signal pathways are present in human preadipocytes, and provides a basis for investigating how Ca 2+ signals regulate biological and physiological activities of human preadipocytes. © 2009 Wiley-Liss, Inc.
 
ISSN0021-9541
2013 Impact Factor: 3.874
 
DOIhttp://dx.doi.org/10.1002/jcp.21823
 
ISI Accession Number IDWOS:000268651500027
Funding AgencyGrant Number
General Research Fund770108M
Research Grant Council of Hong Kong and a CRCG of the University of Hong Kong200811159173
Funding Information:

The present study was supported in part by a General Research Fund (HKU 770108M) from Research Grant Council of Hong Kong and a CRCG seeding grant (200811159173) of the University of Hong Kong.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHu, R
 
dc.contributor.authorHe, ML
 
dc.contributor.authorHu, H
 
dc.contributor.authorYuan, BX
 
dc.contributor.authorZang, WJ
 
dc.contributor.authorLau, CP
 
dc.contributor.authorTse, HF
 
dc.contributor.authorLi, GR
 
dc.date.accessioned2010-09-06T07:38:53Z
 
dc.date.available2010-09-06T07:38:53Z
 
dc.date.issued2009
 
dc.description.abstractIntracellular free Ca 2+ (Ca i 2+) is an important regulator of many cellular activities; however, Ca 2+ signaling is not well studied in human preadipocytes. The purpose of the present study was to characterize Ca 2+ signal pathways using a confocal scanning technique and RT-PCR. It was found that spontaneous Ca i 2+ oscillations were observed in 12.1% preadipocytes, and number of cells withCa 2+ oscillations was increased to 47.9% by 1% fetal bovine serum. Ca i 2+ oscillations were dependent on Ca 2+ entry mainly via stored-operated Ca 2+ (SOC) entry. They were suppressed by the SOC entry channel blocker La 3+, the phospholipase C (PLC) inhibitor U73122, the inositol trisphosphate receptor (IP3R) blocker 2-amino-ethoxydiphenyl borate, or the sarcoplasmic/endoplasmic reticulum Ca 2+ pump (SERCA) inhibitors thapsigargin and cyclopiazonic acid, but not by ryanodine. The IP3R activator thimerosal increased Ca i 2+ oscillations. In addition, the plasma membrane Ca 2+ pump (PMCA) inhibitor carboxyeosin and Na +-Ca 2+ exchanger (NCX) inhibitor Ni 2+ both suppressed Ca 2+ oscillations. RT-PCR revealed that the mRNAs for IP3R1-3, SERCA1,2, NCX3 and PMCA1,3,4, CaV1.2, and TRPC1,4,6, STIM1 and Orai1 (for SOC entry channels) were significant in human preadipocytes. The present study demonstrates that multiple Ca 2+ signal pathways are present in human preadipocytes, and provides a basis for investigating how Ca 2+ signals regulate biological and physiological activities of human preadipocytes. © 2009 Wiley-Liss, Inc.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Cellular Physiology, 2009, v. 220 n. 3, p. 765-770 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jcp.21823
 
dc.identifier.doihttp://dx.doi.org/10.1002/jcp.21823
 
dc.identifier.epage770
 
dc.identifier.hkuros157601
 
dc.identifier.isiWOS:000268651500027
Funding AgencyGrant Number
General Research Fund770108M
Research Grant Council of Hong Kong and a CRCG of the University of Hong Kong200811159173
Funding Information:

The present study was supported in part by a General Research Fund (HKU 770108M) from Research Grant Council of Hong Kong and a CRCG seeding grant (200811159173) of the University of Hong Kong.

 
dc.identifier.issn0021-9541
2013 Impact Factor: 3.874
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.scopuseid_2-s2.0-67650354016
 
dc.identifier.spage765
 
dc.identifier.urihttp://hdl.handle.net/10722/78075
 
dc.identifier.volume220
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Cellular Physiology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Cellular Physiology. Copyright © John Wiley & Sons, Inc.
 
dc.titleCharacterization of calcium signaling pathways in human preadipocytes
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong
  3. Xi'an Jiaotong University