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Article: Characterization of calcium signaling pathways in human preadipocytes

TitleCharacterization of calcium signaling pathways in human preadipocytes
Authors
Issue Date2009
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
Citation
Journal Of Cellular Physiology, 2009, v. 220 n. 3, p. 765-770 How to Cite?
AbstractIntracellular free Ca 2+ (Ca i 2+) is an important regulator of many cellular activities; however, Ca 2+ signaling is not well studied in human preadipocytes. The purpose of the present study was to characterize Ca 2+ signal pathways using a confocal scanning technique and RT-PCR. It was found that spontaneous Ca i 2+ oscillations were observed in 12.1% preadipocytes, and number of cells withCa 2+ oscillations was increased to 47.9% by 1% fetal bovine serum. Ca i 2+ oscillations were dependent on Ca 2+ entry mainly via stored-operated Ca 2+ (SOC) entry. They were suppressed by the SOC entry channel blocker La 3+, the phospholipase C (PLC) inhibitor U73122, the inositol trisphosphate receptor (IP3R) blocker 2-amino-ethoxydiphenyl borate, or the sarcoplasmic/endoplasmic reticulum Ca 2+ pump (SERCA) inhibitors thapsigargin and cyclopiazonic acid, but not by ryanodine. The IP3R activator thimerosal increased Ca i 2+ oscillations. In addition, the plasma membrane Ca 2+ pump (PMCA) inhibitor carboxyeosin and Na +-Ca 2+ exchanger (NCX) inhibitor Ni 2+ both suppressed Ca 2+ oscillations. RT-PCR revealed that the mRNAs for IP3R1-3, SERCA1,2, NCX3 and PMCA1,3,4, CaV1.2, and TRPC1,4,6, STIM1 and Orai1 (for SOC entry channels) were significant in human preadipocytes. The present study demonstrates that multiple Ca 2+ signal pathways are present in human preadipocytes, and provides a basis for investigating how Ca 2+ signals regulate biological and physiological activities of human preadipocytes. © 2009 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/78075
ISSN
2014 Impact Factor: 3.839
2014 SCImago Journal Rankings: 1.528
ISI Accession Number ID
Funding AgencyGrant Number
General Research Fund770108M
Research Grant Council of Hong Kong and a CRCG of the University of Hong Kong200811159173
Funding Information:

The present study was supported in part by a General Research Fund (HKU 770108M) from Research Grant Council of Hong Kong and a CRCG seeding grant (200811159173) of the University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorHu, Ren_HK
dc.contributor.authorHe, MLen_HK
dc.contributor.authorHu, Hen_HK
dc.contributor.authorYuan, BXen_HK
dc.contributor.authorZang, WJen_HK
dc.contributor.authorLau, CPen_HK
dc.contributor.authorTse, HFen_HK
dc.contributor.authorLi, GRen_HK
dc.date.accessioned2010-09-06T07:38:53Z-
dc.date.available2010-09-06T07:38:53Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Cellular Physiology, 2009, v. 220 n. 3, p. 765-770en_HK
dc.identifier.issn0021-9541en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78075-
dc.description.abstractIntracellular free Ca 2+ (Ca i 2+) is an important regulator of many cellular activities; however, Ca 2+ signaling is not well studied in human preadipocytes. The purpose of the present study was to characterize Ca 2+ signal pathways using a confocal scanning technique and RT-PCR. It was found that spontaneous Ca i 2+ oscillations were observed in 12.1% preadipocytes, and number of cells withCa 2+ oscillations was increased to 47.9% by 1% fetal bovine serum. Ca i 2+ oscillations were dependent on Ca 2+ entry mainly via stored-operated Ca 2+ (SOC) entry. They were suppressed by the SOC entry channel blocker La 3+, the phospholipase C (PLC) inhibitor U73122, the inositol trisphosphate receptor (IP3R) blocker 2-amino-ethoxydiphenyl borate, or the sarcoplasmic/endoplasmic reticulum Ca 2+ pump (SERCA) inhibitors thapsigargin and cyclopiazonic acid, but not by ryanodine. The IP3R activator thimerosal increased Ca i 2+ oscillations. In addition, the plasma membrane Ca 2+ pump (PMCA) inhibitor carboxyeosin and Na +-Ca 2+ exchanger (NCX) inhibitor Ni 2+ both suppressed Ca 2+ oscillations. RT-PCR revealed that the mRNAs for IP3R1-3, SERCA1,2, NCX3 and PMCA1,3,4, CaV1.2, and TRPC1,4,6, STIM1 and Orai1 (for SOC entry channels) were significant in human preadipocytes. The present study demonstrates that multiple Ca 2+ signal pathways are present in human preadipocytes, and provides a basis for investigating how Ca 2+ signals regulate biological and physiological activities of human preadipocytes. © 2009 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010en_HK
dc.relation.ispartofJournal of Cellular Physiologyen_HK
dc.rightsJournal of Cellular Physiology. Copyright © John Wiley & Sons, Inc.en_HK
dc.titleCharacterization of calcium signaling pathways in human preadipocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9541&volume=220&issue=3&spage=765&epage=770&date=2009&atitle=Characterization+of+calcium+signaling+pathways+in+human+preadipocytesen_HK
dc.identifier.emailTse, HF:hftse@hkucc.hku.hken_HK
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jcp.21823en_HK
dc.identifier.scopuseid_2-s2.0-67650354016en_HK
dc.identifier.hkuros157601en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67650354016&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume220en_HK
dc.identifier.issue3en_HK
dc.identifier.spage765en_HK
dc.identifier.epage770en_HK
dc.identifier.isiWOS:000268651500027-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHu, R=55163131900en_HK
dc.identifier.scopusauthoridHe, ML=7402609178en_HK
dc.identifier.scopusauthoridHu, H=36812244900en_HK
dc.identifier.scopusauthoridYuan, BX=7203056413en_HK
dc.identifier.scopusauthoridZang, WJ=7005740494en_HK
dc.identifier.scopusauthoridLau, CP=7401968501en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.scopusauthoridLi, GR=7408462932en_HK

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