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Article: Fasting and postprandial determinants for the occurrence of small dense LDL species in non-insulin-dependent diabetic patients with and without hypertriglyceridaemia: The involvement of insulin, insulin precursor species and insulin resistance

TitleFasting and postprandial determinants for the occurrence of small dense LDL species in non-insulin-dependent diabetic patients with and without hypertriglyceridaemia: The involvement of insulin, insulin precursor species and insulin resistance
Authors
Issue Date1995
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/atherosclerosis
Citation
Atherosclerosis, 1995, v. 113 n. 2, p. 273-287 How to Cite?
AbstractWe have studied low density lipoprotein (LDL) subclass distribution in a group of male patients with non-insulin-dependent diabetes mellitus (NIDDM) and investigated its relationships to fasting and postprandial triglyceride (TG)-rich lipoproteins, insulin resistance, lipoprotein lipase (EC 3.1.1.3; LPL), hepatic lipase (EC 3.2.1.34; HL), lecithin:cholesterol acyl transferase (EC 2.3.1.43; LCAT) and cholesteryl ester transfer protein (CETP) activities. LDL was subfractionated by density gradient ultracentrifugation. Postprandial lipoproteins were measured after an oral fat load using retinyl palmitate as a marker for intestinal TG-rich lipoproteins. Hypertriglyceridaemic NIDDMs (HTG) had a preponderance of small dense LDL particles present in the plasma and reduced amounts of large buoyant species when compared to normotriglyceridaemic patients (NTG) and controls. Both groups of diabetics were more insulin resistant than the controls (P < 0.05) and had raised concentrations of proinsulin (P < 0.05), although insulin content did not differ significantly. 32-33 split proinsulin (SPI) was the major insulin-like molecule present in HTG and was present in significantly higher amounts in these patients (P < 0.05) than either NTG or control subjects and correlated significantly with the presence of small dense LDL particles, After a test meal, the postprandial chylomicron response was greater in HTG than either NTG diabetics or controls (P < 0.05). Chylomicron remnants were present to a greater extent in HTG than in NTG and controls (P < 0.05), although in this case NTG also contained more chylomicron remnants than control subjects (P < 0.05). There was no difference in the LPL activity, CETP and LCAT between diabetics and controls, whereas an increase in hepatic lipase activity was seen in the HTG diabetics (P < 0.05). Both CETP and LCAT activities increased postprandially. Multivariate analysis showed that TG, HDL content and HL activity were the most important determinants of small dense LDL concentration in the fasting state (R2 = 67%). Postprandially, chylomicron remnant clearance, HL and insulin resistance were the major determinants (R2 = 61%) of LDL-III.
Persistent Identifierhttp://hdl.handle.net/10722/78027
ISSN
2015 Impact Factor: 3.942
2015 SCImago Journal Rankings: 1.819
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTan, KCBen_HK
dc.contributor.authorCooper, MBen_HK
dc.contributor.authorLing, KLEen_HK
dc.contributor.authorGriffin, BAen_HK
dc.contributor.authorFreeman, DJen_HK
dc.contributor.authorPackard, CJen_HK
dc.contributor.authorShepherd, Jen_HK
dc.contributor.authorHales, CNen_HK
dc.contributor.authorBetteridge, DJen_HK
dc.date.accessioned2010-09-06T07:38:22Z-
dc.date.available2010-09-06T07:38:22Z-
dc.date.issued1995en_HK
dc.identifier.citationAtherosclerosis, 1995, v. 113 n. 2, p. 273-287en_HK
dc.identifier.issn0021-9150en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78027-
dc.description.abstractWe have studied low density lipoprotein (LDL) subclass distribution in a group of male patients with non-insulin-dependent diabetes mellitus (NIDDM) and investigated its relationships to fasting and postprandial triglyceride (TG)-rich lipoproteins, insulin resistance, lipoprotein lipase (EC 3.1.1.3; LPL), hepatic lipase (EC 3.2.1.34; HL), lecithin:cholesterol acyl transferase (EC 2.3.1.43; LCAT) and cholesteryl ester transfer protein (CETP) activities. LDL was subfractionated by density gradient ultracentrifugation. Postprandial lipoproteins were measured after an oral fat load using retinyl palmitate as a marker for intestinal TG-rich lipoproteins. Hypertriglyceridaemic NIDDMs (HTG) had a preponderance of small dense LDL particles present in the plasma and reduced amounts of large buoyant species when compared to normotriglyceridaemic patients (NTG) and controls. Both groups of diabetics were more insulin resistant than the controls (P < 0.05) and had raised concentrations of proinsulin (P < 0.05), although insulin content did not differ significantly. 32-33 split proinsulin (SPI) was the major insulin-like molecule present in HTG and was present in significantly higher amounts in these patients (P < 0.05) than either NTG or control subjects and correlated significantly with the presence of small dense LDL particles, After a test meal, the postprandial chylomicron response was greater in HTG than either NTG diabetics or controls (P < 0.05). Chylomicron remnants were present to a greater extent in HTG than in NTG and controls (P < 0.05), although in this case NTG also contained more chylomicron remnants than control subjects (P < 0.05). There was no difference in the LPL activity, CETP and LCAT between diabetics and controls, whereas an increase in hepatic lipase activity was seen in the HTG diabetics (P < 0.05). Both CETP and LCAT activities increased postprandially. Multivariate analysis showed that TG, HDL content and HL activity were the most important determinants of small dense LDL concentration in the fasting state (R2 = 67%). Postprandially, chylomicron remnant clearance, HL and insulin resistance were the major determinants (R2 = 61%) of LDL-III.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/atherosclerosisen_HK
dc.relation.ispartofAtherosclerosisen_HK
dc.rightsAtherosclerosis. Copyright © Elsevier Ireland Ltd.en_HK
dc.subject.meshCase-Control Studiesen_HK
dc.subject.meshCentrifugation, Density Gradienten_HK
dc.subject.meshDiabetes Mellitus, Type 2 - blood - complicationsen_HK
dc.subject.meshEating - physiologyen_HK
dc.subject.meshFasting - blooden_HK
dc.subject.meshHumansen_HK
dc.subject.meshHypertriglyceridemia - blood - complicationsen_HK
dc.subject.meshInsulin - blooden_HK
dc.subject.meshInsulin Resistance - physiologyen_HK
dc.subject.meshLipase - blooden_HK
dc.subject.meshLipoproteins, LDL - blooden_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshMultivariate Analysisen_HK
dc.subject.meshPhosphatidylcholines - blooden_HK
dc.subject.meshProinsulin - blooden_HK
dc.subject.meshRadioimmunoassayen_HK
dc.titleFasting and postprandial determinants for the occurrence of small dense LDL species in non-insulin-dependent diabetic patients with and without hypertriglyceridaemia: The involvement of insulin, insulin precursor species and insulin resistanceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9150&volume=133&spage=273&epage=287&date=1995&atitle=Fasting+and+postprandial+determinants+for+the+occurrence+of+small+dense+LDL+species+in+non-insulin-dependent+diabetic+patients+with+and+without+hypertriglyceridaemia:+the+involvement+of+insulin,+insulin+precursor+species+and+insulin+resistanceen_HK
dc.identifier.emailTan, KCB:kcbtan@hku.hken_HK
dc.identifier.authorityTan, KCB=rp00402en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/0021-9150(94)05454-Qen_HK
dc.identifier.pmid7605366-
dc.identifier.scopuseid_2-s2.0-0028959903en_HK
dc.identifier.hkuros4773en_HK
dc.identifier.volume113en_HK
dc.identifier.issue2en_HK
dc.identifier.spage273en_HK
dc.identifier.epage287en_HK
dc.identifier.isiWOS:A1995QQ13600015-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridTan, KCB=8082703100en_HK
dc.identifier.scopusauthoridCooper, MB=7404410514en_HK
dc.identifier.scopusauthoridLing, KLE=35911069500en_HK
dc.identifier.scopusauthoridGriffin, BA=7201402675en_HK
dc.identifier.scopusauthoridFreeman, DJ=7402382610en_HK
dc.identifier.scopusauthoridPackard, CJ=7103089573en_HK
dc.identifier.scopusauthoridShepherd, J=7401741461en_HK
dc.identifier.scopusauthoridHales, CN=7103213698en_HK
dc.identifier.scopusauthoridBetteridge, DJ=34973752700en_HK

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