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Article: Preferential type1-1 cytokine gene expressions in peripheral T-cell lymphomas

TitlePreferential type1-1 cytokine gene expressions in peripheral T-cell lymphomas
Authors
KeywordsCytokine gene expression
Tumour environment
Type 1
Type 2
Issue Date1999
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/3182
Citation
Hematological Oncology, 1999, v. 17 n. 3, p. 117-129 How to Cite?
AbstractIn this study, we have investigated whether a pattern of cytokine gene expression can be found in non-Hodgkin's peripheral T-cell lymphoma (PTCL). By using RNase protection assays and RT-PCR, we have systematically studied IL1α, IL1β, IL1-Ra, IL2, IL4, IL5, IL6, IL9, IL10, IL12p35, IL12p40, IL13, IL14, IL15, IFNγ, IFNβ, TNFα, TNFβ, LTβ, and TGFβ1, TGFβ2 and TGFβ3. Twenty-two cases of PTCL inclusive of three nasal NK-cell lymphomas were selected for the study; three cases of reactive lymphoproliferation were included for comparison. Results show that IFNγ gene expression (key Type 1 cytokine) was frequently detected [18/22 (82 per cent)]. In contrast, IL4 (key Type 2 cytokine) was only detected in 4/22 (18 per cent) of cases (weaker than IFNγ in three cases). This distinction was also found at the protein level by immunohistochemistry. In addition, TNFβ and TNFα (strongly expressed by Type 1 cells) were almost complimentarily detected [4/19 (21 per cent)] and 12/19 (63 per cent), respectively). In contrast, neither IL5 nor IL13 (strongly expressed by Type 2 cells) were detected at all. However, 14/22 cases expressed IL10, another Type 2 cytokine, which suggests that the autoregulatory feedback loop is stimulated. Compared to the tumour types, the cytokine profiles in the reactive lymphoproliferative types also resembled a Type 1-like pattern but was less striking. The overall result suggested a preferential expression of certain cytokines, and these cytokines may play an important role in pathophysiologic progression in these T-cell disorders. Copyright (C) 1999 John Wiley and Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/78016
ISSN
2015 Impact Factor: 3.494
2015 SCImago Journal Rankings: 0.767
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, JWYen_HK
dc.contributor.authorLiang, RHSen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.date.accessioned2010-09-06T07:38:14Z-
dc.date.available2010-09-06T07:38:14Z-
dc.date.issued1999en_HK
dc.identifier.citationHematological Oncology, 1999, v. 17 n. 3, p. 117-129en_HK
dc.identifier.issn0278-0232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78016-
dc.description.abstractIn this study, we have investigated whether a pattern of cytokine gene expression can be found in non-Hodgkin's peripheral T-cell lymphoma (PTCL). By using RNase protection assays and RT-PCR, we have systematically studied IL1α, IL1β, IL1-Ra, IL2, IL4, IL5, IL6, IL9, IL10, IL12p35, IL12p40, IL13, IL14, IL15, IFNγ, IFNβ, TNFα, TNFβ, LTβ, and TGFβ1, TGFβ2 and TGFβ3. Twenty-two cases of PTCL inclusive of three nasal NK-cell lymphomas were selected for the study; three cases of reactive lymphoproliferation were included for comparison. Results show that IFNγ gene expression (key Type 1 cytokine) was frequently detected [18/22 (82 per cent)]. In contrast, IL4 (key Type 2 cytokine) was only detected in 4/22 (18 per cent) of cases (weaker than IFNγ in three cases). This distinction was also found at the protein level by immunohistochemistry. In addition, TNFβ and TNFα (strongly expressed by Type 1 cells) were almost complimentarily detected [4/19 (21 per cent)] and 12/19 (63 per cent), respectively). In contrast, neither IL5 nor IL13 (strongly expressed by Type 2 cells) were detected at all. However, 14/22 cases expressed IL10, another Type 2 cytokine, which suggests that the autoregulatory feedback loop is stimulated. Compared to the tumour types, the cytokine profiles in the reactive lymphoproliferative types also resembled a Type 1-like pattern but was less striking. The overall result suggested a preferential expression of certain cytokines, and these cytokines may play an important role in pathophysiologic progression in these T-cell disorders. Copyright (C) 1999 John Wiley and Sons, Ltd.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/3182en_HK
dc.relation.ispartofHematological Oncologyen_HK
dc.rightsHematological Oncology. Copyright © John Wiley & Sons Ltd.en_HK
dc.subjectCytokine gene expressionen_HK
dc.subjectTumour environmenten_HK
dc.subjectType 1en_HK
dc.subjectType 2en_HK
dc.subject.meshCytokines - genetics - metabolismen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInterferons - genetics - metabolismen_HK
dc.subject.meshInterleukins - genetics - metabolismen_HK
dc.subject.meshKiller Cells, Natural - metabolismen_HK
dc.subject.meshLymphocyte Subsets - metabolismen_HK
dc.subject.meshLymphoma, T-Cell, Peripheral - genetics - metabolism - pathologyen_HK
dc.subject.meshPhenotypeen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshT-Lymphocytes - metabolismen_HK
dc.subject.meshTumor Necrosis Factor-alpha - genetics - metabolismen_HK
dc.titlePreferential type1-1 cytokine gene expressions in peripheral T-cell lymphomasen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0278-0232&volume=17&spage=117&epage=129&date=1999&atitle=Preferential+type1-1+cytokine+gene+expressions+in+peripheral+T-cell+lymphomasen_HK
dc.identifier.emailLiang, RHS:rliang@hku.hken_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authorityLiang, RHS=rp00345en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1099-1069(199909)17:3<117::AID-HON640>3.0.CO;2-1en_HK
dc.identifier.pmid10641032-
dc.identifier.scopuseid_2-s2.0-0033233239en_HK
dc.identifier.hkuros52626en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033233239&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue3en_HK
dc.identifier.spage117en_HK
dc.identifier.epage129en_HK
dc.identifier.isiWOS:000085252900003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHo, JWY=7402649982en_HK
dc.identifier.scopusauthoridLiang, RHS=26643224900en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK

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