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Article: Activation of the caspase-8/Bid and Bax pathways in aspirin-induced apoptosis in gastric cancer
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TitleActivation of the caspase-8/Bid and Bax pathways in aspirin-induced apoptosis in gastric cancer
 
AuthorsGu, Q2 1
De Wang, J1
Xia, HHX1
Lin, MCM1
He, H1
Zou, B1
Tu, SP1
Yang, Y1
Liu, XG2
Lam, SK1
Wong, WM1
Chan, AOO1
Yuen, MF1
Kung, HF1
Wong, BCY1
 
Issue Date2005
 
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
 
CitationCarcinogenesis, 2005, v. 26 n. 3, p. 541-546 [How to Cite?]
DOI: http://dx.doi.org/10.1093/carcin/bgh345
 
AbstractAspirin-induced apoptosis is one of the important mechanisms for its antitumour effect against gastric cancer. We aimed at investigating the involvement of bcl-2 family members in the apoptotic pathway in gastric cancer. Gastric cancer cell line AGS and MKN-45 were observed as to cell growth inhibition and induction of apoptosis in response to treatment with aspirin. Cell proliferation was measured by MTT assay. Apoptosis was determined by 4′-6-diamidino-2-phenylindole staining. Protein expression was determined by western blotting. We showed that aspirin activated caspase-8, caspase-9 and capase-3, cleaved and translocated Bid, induced a conformational change in and translocation of Bax and cytochrome c release. In addition, suppression of caspase-8 with the specific inhibitor z-IETD-fmk, as well as the pan-caspase inhibitor z-VAD-fmk, prevented Bid cleavage and subsequent apoptosis. The caspase inhibitors failed to abolish the effects on Bax activation. In conclusion, our results identify a role of caspase-8/Bid and activation of Bax as a novel mechanism for aspirin-induced apoptosis in gastric cancer. © Oxford University Press 2004; all rights reserved.
 
ISSN0143-3334
2012 Impact Factor: 5.635
2012 SCImago Journal Rankings: 2.349
 
DOIhttp://dx.doi.org/10.1093/carcin/bgh345
 
ISI Accession Number IDWOS:000227242400004
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorGu, Q
 
dc.contributor.authorDe Wang, J
 
dc.contributor.authorXia, HHX
 
dc.contributor.authorLin, MCM
 
dc.contributor.authorHe, H
 
dc.contributor.authorZou, B
 
dc.contributor.authorTu, SP
 
dc.contributor.authorYang, Y
 
dc.contributor.authorLiu, XG
 
dc.contributor.authorLam, SK
 
dc.contributor.authorWong, WM
 
dc.contributor.authorChan, AOO
 
dc.contributor.authorYuen, MF
 
dc.contributor.authorKung, HF
 
dc.contributor.authorWong, BCY
 
dc.date.accessioned2010-09-06T07:38:07Z
 
dc.date.available2010-09-06T07:38:07Z
 
dc.date.issued2005
 
dc.description.abstractAspirin-induced apoptosis is one of the important mechanisms for its antitumour effect against gastric cancer. We aimed at investigating the involvement of bcl-2 family members in the apoptotic pathway in gastric cancer. Gastric cancer cell line AGS and MKN-45 were observed as to cell growth inhibition and induction of apoptosis in response to treatment with aspirin. Cell proliferation was measured by MTT assay. Apoptosis was determined by 4′-6-diamidino-2-phenylindole staining. Protein expression was determined by western blotting. We showed that aspirin activated caspase-8, caspase-9 and capase-3, cleaved and translocated Bid, induced a conformational change in and translocation of Bax and cytochrome c release. In addition, suppression of caspase-8 with the specific inhibitor z-IETD-fmk, as well as the pan-caspase inhibitor z-VAD-fmk, prevented Bid cleavage and subsequent apoptosis. The caspase inhibitors failed to abolish the effects on Bax activation. In conclusion, our results identify a role of caspase-8/Bid and activation of Bax as a novel mechanism for aspirin-induced apoptosis in gastric cancer. © Oxford University Press 2004; all rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationCarcinogenesis, 2005, v. 26 n. 3, p. 541-546 [How to Cite?]
DOI: http://dx.doi.org/10.1093/carcin/bgh345
 
dc.identifier.citeulike104748
 
dc.identifier.doihttp://dx.doi.org/10.1093/carcin/bgh345
 
dc.identifier.epage546
 
dc.identifier.hkuros97452
 
dc.identifier.isiWOS:000227242400004
 
dc.identifier.issn0143-3334
2012 Impact Factor: 5.635
2012 SCImago Journal Rankings: 2.349
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.scopuseid_2-s2.0-16244417810
 
dc.identifier.spage541
 
dc.identifier.urihttp://hdl.handle.net/10722/78004
 
dc.identifier.volume26
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofCarcinogenesis
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCarcinogenesis. Copyright © Oxford University Press.
 
dc.titleActivation of the caspase-8/Bid and Bax pathways in aspirin-induced apoptosis in gastric cancer
 
dc.typeArticle
 
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<contributor.author>Chan, AOO</contributor.author>
<contributor.author>Yuen, MF</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Peking University