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Article: Metabolism of chloramphenicol succinate in human bone marrow

TitleMetabolism of chloramphenicol succinate in human bone marrow
Authors
KeywordsChloramphenicol
Chloramphenicol succinate
Human bone marrow
Issue Date2000
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00228/index.htm
Citation
European Journal Of Clinical Pharmacology, 2000, v. 56 n. 5, p. 405-409 How to Cite?
AbstractObjective/methods: The metabolism of chloramphenicol succinate (CAPS) by human bone marrow was studied in vitro using 75 marrow samples. Whole marrow samples were incubated with CAPS with or without reduced nicotinamide adenine dinucleotide phosphate for 1, 2 and 3 h at 37 °C. Ficoll-paque-separated marrow mononuclear cells and erythrocytes were similarly incubated. After precipitation and centrifugation, clear supernatant was analysed for the presence of metabolites using high-performance liquid chromatography. Results: Only one metabolite was detected when CAPS was incubated for 3 h with whole marrow from 72 donors. Its retention time (RT 10.9 min) corresponded to chloramphenicol (CAP). When CAPS was incubated with samples of whole marrow, marrow mononuclear cells, marrow erythrocytes, marrow plasma and peripheral blood from one donor who had taken Traditional Chinese Medicine (TCM), three metabolite peaks were detected within 15 min to 1 h. The RT of two of these peaks corresponded to CAP and nitroso-CAP (RT 14.9 min), but one peak remained unidentified. These peaks were not detected in the control samples incubated without CAPS. Blood samples collected after 3 months and 6 months to reconfirm metabolic activity yielded no such metabolite peaks when incubated with CAPS for 1-3 h. Therefore induction of enzyme activity by TCM was suspected. Three metabolite peaks with the same RTs were also detected when CAPS was incubated for 3 h with whole marrow from two other donors. Conclusion: These studies demonstrated that CAPS may be metabolised to CAP and occasionally other metabolites in human bone marrow. This novel observation is particularly important because the bone marrow is known to be a target organ for chloramphenicol toxicity.
Persistent Identifierhttp://hdl.handle.net/10722/77956
ISSN
2015 Impact Factor: 2.71
2015 SCImago Journal Rankings: 1.096
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAmbekar, CSen_HK
dc.contributor.authorCheung, Ben_HK
dc.contributor.authorLee, Jen_HK
dc.contributor.authorChan, LCen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorKumana, CRen_HK
dc.date.accessioned2010-09-06T07:37:36Z-
dc.date.available2010-09-06T07:37:36Z-
dc.date.issued2000en_HK
dc.identifier.citationEuropean Journal Of Clinical Pharmacology, 2000, v. 56 n. 5, p. 405-409en_HK
dc.identifier.issn0031-6970en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77956-
dc.description.abstractObjective/methods: The metabolism of chloramphenicol succinate (CAPS) by human bone marrow was studied in vitro using 75 marrow samples. Whole marrow samples were incubated with CAPS with or without reduced nicotinamide adenine dinucleotide phosphate for 1, 2 and 3 h at 37 °C. Ficoll-paque-separated marrow mononuclear cells and erythrocytes were similarly incubated. After precipitation and centrifugation, clear supernatant was analysed for the presence of metabolites using high-performance liquid chromatography. Results: Only one metabolite was detected when CAPS was incubated for 3 h with whole marrow from 72 donors. Its retention time (RT 10.9 min) corresponded to chloramphenicol (CAP). When CAPS was incubated with samples of whole marrow, marrow mononuclear cells, marrow erythrocytes, marrow plasma and peripheral blood from one donor who had taken Traditional Chinese Medicine (TCM), three metabolite peaks were detected within 15 min to 1 h. The RT of two of these peaks corresponded to CAP and nitroso-CAP (RT 14.9 min), but one peak remained unidentified. These peaks were not detected in the control samples incubated without CAPS. Blood samples collected after 3 months and 6 months to reconfirm metabolic activity yielded no such metabolite peaks when incubated with CAPS for 1-3 h. Therefore induction of enzyme activity by TCM was suspected. Three metabolite peaks with the same RTs were also detected when CAPS was incubated for 3 h with whole marrow from two other donors. Conclusion: These studies demonstrated that CAPS may be metabolised to CAP and occasionally other metabolites in human bone marrow. This novel observation is particularly important because the bone marrow is known to be a target organ for chloramphenicol toxicity.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00228/index.htmen_HK
dc.relation.ispartofEuropean Journal of Clinical Pharmacologyen_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectChloramphenicolen_HK
dc.subjectChloramphenicol succinateen_HK
dc.subjectHuman bone marrowen_HK
dc.subject.meshBone Marrow - drug effects - metabolism-
dc.subject.meshCells, Cultured-
dc.subject.meshChloramphenicol - analogs and derivatives - metabolism-
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshHumans-
dc.titleMetabolism of chloramphenicol succinate in human bone marrowen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0031-6970&volume=56&issue=5&spage=405&epage=409&date=2000&atitle=Metabolism+of+chloramphenicol+succinate+in+human+bone+marrowen_HK
dc.identifier.emailCheung, B:mycheung@hku.hken_HK
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.authorityCheung, B=rp01321en_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11009050-
dc.identifier.scopuseid_2-s2.0-0033844475en_HK
dc.identifier.hkuros56475en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033844475&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume56en_HK
dc.identifier.issue5en_HK
dc.identifier.spage405en_HK
dc.identifier.epage409en_HK
dc.identifier.isiWOS:000089092800010-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridAmbekar, CS=55278609200en_HK
dc.identifier.scopusauthoridCheung, B=7103294806en_HK
dc.identifier.scopusauthoridLee, J=7601479992en_HK
dc.identifier.scopusauthoridChan, LC=7403540707en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridKumana, CR=7005112381en_HK

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