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Article: Prevalence of CagA-bearing Helicobacter pylori strains detected by the anti-CagA assay in patients with peptic ulcer disease and in controls

TitlePrevalence of CagA-bearing Helicobacter pylori strains detected by the anti-CagA assay in patients with peptic ulcer disease and in controls
Authors
Issue Date1996
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ajg/index.html
Citation
American Journal Of Gastroenterology, 1996, v. 91 n. 5, p. 949-953 How to Cite?
AbstractObjective: Cytotoxin-associated gene (CagA)-bearing Helicobacter pylori strains have been associated with significant gastroduodenal pathologies. We have performed a study to evaluate the prevalence of CagA-bearing strains in a group of H. pylori-positive peptic ulcer disease and non-ulcer dyspepsia (NUD) patients, and healthy asymptomatic controls. Method: Two hundred ninety-seven peptic ulcer disease, 45 NUD subjects, and 200 asymptomatic controls were studied. The newly developed anti-CagA antibody assay was used for the purpose of this study. The assay was performed by a conventional three-step enzyme-linked immunosorbent assay (ELISA) to detect the concentration of anti-CagA antibody present in the tested sera against the recombinant CagA 17/12 fusion protein. The final results were expressed with reference to a standard curve constructed from pooled CagA+ sera. Anti-CagA antibody assay reproducibility was assessed by intraplate and interplate variations. Results: The mean intraplate and interplate variations were 8.0% and 11.2%, respectively. Anti-CagA antibody was present in 165/197 (84%) duodenal ulcer disease, 80/100 (80%) gastric ulcer disease, 25/45 (55.6%) NUD subjects, and 29/100 (29%) asymptomatic controls. The ulcer disease subjects were significantly more likely than the NUD subjects and the asymptomatic controls to have a positive anti-CagA antibody assay (p < 0.005 and p < 0.001, respectively). Moreover, the NUD subjects were more likely to be anti- CagA+ than the asymptomatic controls (p < 0.005). Conclusions: This newly developed anti-CagA antibody assay was highly reproducible. Anti-CagA antibody positivity was present in a significantly higher percentage of peptic ulcer disease subjects than in non-ulcer and asymptomatic healthy controls. Thus, anti-CagA antibody can be used as a clinical marker for peptic ulceration.
Persistent Identifierhttp://hdl.handle.net/10722/77724
ISSN
2015 Impact Factor: 10.383
2015 SCImago Journal Rankings: 3.946
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChing, CKen_HK
dc.contributor.authorWong, BCYen_HK
dc.contributor.authorKwok, Een_HK
dc.contributor.authorOng, Len_HK
dc.contributor.authorCovacci, Aen_HK
dc.contributor.authorLam, SKen_HK
dc.date.accessioned2010-09-06T07:35:02Z-
dc.date.available2010-09-06T07:35:02Z-
dc.date.issued1996en_HK
dc.identifier.citationAmerican Journal Of Gastroenterology, 1996, v. 91 n. 5, p. 949-953en_HK
dc.identifier.issn0002-9270en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77724-
dc.description.abstractObjective: Cytotoxin-associated gene (CagA)-bearing Helicobacter pylori strains have been associated with significant gastroduodenal pathologies. We have performed a study to evaluate the prevalence of CagA-bearing strains in a group of H. pylori-positive peptic ulcer disease and non-ulcer dyspepsia (NUD) patients, and healthy asymptomatic controls. Method: Two hundred ninety-seven peptic ulcer disease, 45 NUD subjects, and 200 asymptomatic controls were studied. The newly developed anti-CagA antibody assay was used for the purpose of this study. The assay was performed by a conventional three-step enzyme-linked immunosorbent assay (ELISA) to detect the concentration of anti-CagA antibody present in the tested sera against the recombinant CagA 17/12 fusion protein. The final results were expressed with reference to a standard curve constructed from pooled CagA+ sera. Anti-CagA antibody assay reproducibility was assessed by intraplate and interplate variations. Results: The mean intraplate and interplate variations were 8.0% and 11.2%, respectively. Anti-CagA antibody was present in 165/197 (84%) duodenal ulcer disease, 80/100 (80%) gastric ulcer disease, 25/45 (55.6%) NUD subjects, and 29/100 (29%) asymptomatic controls. The ulcer disease subjects were significantly more likely than the NUD subjects and the asymptomatic controls to have a positive anti-CagA antibody assay (p < 0.005 and p < 0.001, respectively). Moreover, the NUD subjects were more likely to be anti- CagA+ than the asymptomatic controls (p < 0.005). Conclusions: This newly developed anti-CagA antibody assay was highly reproducible. Anti-CagA antibody positivity was present in a significantly higher percentage of peptic ulcer disease subjects than in non-ulcer and asymptomatic healthy controls. Thus, anti-CagA antibody can be used as a clinical marker for peptic ulceration.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ajg/index.htmlen_HK
dc.relation.ispartofAmerican Journal of Gastroenterologyen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAntibodies - analysisen_HK
dc.subject.meshCohort Studiesen_HK
dc.subject.meshCytotoxins - genetics - immunologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHelicobacter pylori - genetics - isolation & purificationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPeptic Ulcer - microbiologyen_HK
dc.subject.meshReference Valuesen_HK
dc.subject.meshReproducibility of Resultsen_HK
dc.titlePrevalence of CagA-bearing Helicobacter pylori strains detected by the anti-CagA assay in patients with peptic ulcer disease and in controlsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0002-9270&volume=91&spage=949&epage=953&date=1996&atitle=Prevalence+of+CagA-bearing+Helicobacter+pylori+strains+detected+by+the+anti-CagA+assay+in+patients+with+peptic+ulcer+disease+and+in+controlsen_HK
dc.identifier.emailWong, BCY:bcywong@hku.hken_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8633586-
dc.identifier.scopuseid_2-s2.0-0029974653en_HK
dc.identifier.hkuros29670en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029974653&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume91en_HK
dc.identifier.issue5en_HK
dc.identifier.spage949en_HK
dc.identifier.epage953en_HK
dc.identifier.isiWOS:A1996UJ97500022-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChing, CK=7102130825en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK
dc.identifier.scopusauthoridKwok, E=7005571440en_HK
dc.identifier.scopusauthoridOng, L=8091873900en_HK
dc.identifier.scopusauthoridCovacci, A=7003304561en_HK
dc.identifier.scopusauthoridLam, SK=7402279473en_HK

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