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Article: Adenoviral-mediated suicide gene therapy for hepatic metastases of breast cancer

TitleAdenoviral-mediated suicide gene therapy for hepatic metastases of breast cancer
Authors
Issue Date1996
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/cgt
Citation
Cancer Gene Therapy, 1996, v. 3 n. 5, p. 339-344 How to Cite?
AbstractMetastases of breast cancer are a major cause of treatment failure. To evaluate the therapeutic efficacy of suicide gene therapy in metastatic breast cancer, we used the herpes simplex virus thymidine kinase (HSV-tk) gene followed by ganciclovir (GCV) administration to treat breast cancer, generated by an adenocarcinoma cell line MOD in syngeneic mice. The bystander effect of HSV-tk + GCV on tumor cell killing was illustrated by demonstrating complete regression of subcutaneous tumors consisting of 90% parental tumor cells and 10% HSV-tk transformed tumor cells. To establish a model of breast cancer metastases in the liver, tumors were generated by intra-hepatic implantation of MOD cells in syngeneic animals. Two weeks after tumor cell implantation, replication defective adenoviral vectors expressing HSV-tk (ADV.tk), or beta-galactosidease (ADV. beta-Gal) were injected intratumorally, followed by buffer or GCV administration. Treatment with ADV.tk + GCV resulted in significant regression of tumor (P < .001), as assessed by computerized morphometric analysis of residual tumor. This was reflected as a significant prolongation of survival in treated animals (P < .001). These results demonstrate that ADV-mediated suicide gene therapy in vivo can be incorporated in a comprehensive treatment strategy for liver metastases of breast cancer.
Persistent Identifierhttp://hdl.handle.net/10722/77695
ISSN
2015 Impact Factor: 2.532
2015 SCImago Journal Rankings: 1.020
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorChen, SHen_HK
dc.contributor.authorKosai, Ken_HK
dc.contributor.authorFinegold, MJen_HK
dc.contributor.authorWoo, SLC-
dc.date.accessioned2010-09-06T07:34:42Z-
dc.date.available2010-09-06T07:34:42Z-
dc.date.issued1996en_HK
dc.identifier.citationCancer Gene Therapy, 1996, v. 3 n. 5, p. 339-344en_HK
dc.identifier.issn0929-1903en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77695-
dc.description.abstractMetastases of breast cancer are a major cause of treatment failure. To evaluate the therapeutic efficacy of suicide gene therapy in metastatic breast cancer, we used the herpes simplex virus thymidine kinase (HSV-tk) gene followed by ganciclovir (GCV) administration to treat breast cancer, generated by an adenocarcinoma cell line MOD in syngeneic mice. The bystander effect of HSV-tk + GCV on tumor cell killing was illustrated by demonstrating complete regression of subcutaneous tumors consisting of 90% parental tumor cells and 10% HSV-tk transformed tumor cells. To establish a model of breast cancer metastases in the liver, tumors were generated by intra-hepatic implantation of MOD cells in syngeneic animals. Two weeks after tumor cell implantation, replication defective adenoviral vectors expressing HSV-tk (ADV.tk), or beta-galactosidease (ADV. beta-Gal) were injected intratumorally, followed by buffer or GCV administration. Treatment with ADV.tk + GCV resulted in significant regression of tumor (P < .001), as assessed by computerized morphometric analysis of residual tumor. This was reflected as a significant prolongation of survival in treated animals (P < .001). These results demonstrate that ADV-mediated suicide gene therapy in vivo can be incorporated in a comprehensive treatment strategy for liver metastases of breast cancer.-
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/cgten_HK
dc.relation.ispartofCancer Gene Therapyen_HK
dc.subject.meshAdenoviridae - genetics-
dc.subject.meshBreast Neoplasms - metabolism-
dc.subject.meshGenetic Therapy-
dc.subject.meshLiver Neoplasms, Experimental - secondary - therapy-
dc.subject.meshNeoplasm Metastasis-
dc.titleAdenoviral-mediated suicide gene therapy for hepatic metastases of breast canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0929-1903&volume=3&spage=339&epage=344&date=1996&atitle=Adenoviral+mediated+suicide+gene+therapy+for+hepatic+metastases+of+carcinoma+of+the+breast.en_HK
dc.identifier.emailKwong, YL: ylkwong@hku.hken_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.pmid8894253-
dc.identifier.scopuseid_2-s2.0-0030227835-
dc.identifier.hkuros24178en_HK
dc.identifier.volume3-
dc.identifier.issue5-
dc.identifier.spage339-
dc.identifier.epage344-
dc.identifier.isiWOS:A1996VM73800007-
dc.publisher.placeUnited Kingdom-

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