File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Coculture with primary visceral rat adipocytes from control but not streptozotocin-induced diabetic animals increases glucose uptake in rat skeletal muscle cells: Role of adiponectin

TitleCoculture with primary visceral rat adipocytes from control but not streptozotocin-induced diabetic animals increases glucose uptake in rat skeletal muscle cells: Role of adiponectin
Authors
Issue Date2007
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2007, v. 148 n. 9, p. 4411-4419 How to Cite?
AbstractWe developed a coculture system comprising primary rat adipocytes and L6 rat skeletal muscle cells to allow investigation of the effects of physiologically relevant mixtures of adipokines. We observed that coculture, or adipocyte-conditioned media, increased glucose uptake in muscle cells. An adipokine that could potentially mediate this effect is adiponectin, and wedemonstrated that small interfering RNA-mediated knock-down of adiponectin receptor-2 in muscle cells reduced the uptake of glucose upon coculture with primary rat adipocytes. Analysis of coculture media by ELISA indicated total adiponectin concentration of up to 1 μg/ml, and Western blotting and gel filtration analysis demonstrated that the adipokine profile was hexamer greater than high molecular weight much greater than trimer. We used the streptozotocin-induced rat model of diabetes and found that high-molecularweight adiponectin levels decreased in comparison with control animals and this correlated with the fact that diabetic rat-derived primary adipocytes in coculture did not stimulate glucose uptake to the same extent as control adipocytes. Coculture induced phosphorylation of AMP-activated protein kinase (T172) and interestingly also insulin receptor substrate-1 (Y612) and Akt (T308 & S473), which could be attenuated after adiponectin receptor-2-small interfering RNA treatment. In summary, we believe that this coculture system represents an excellent model to study the effects of primary adipocyte-derived adipokine mixtures on skeletal muscle metabolism, and here we have established that in the context of physiologically relevant mixtures of adipokines, adiponectin may be an important determinant of positive cross talk between adipocytes and skeletal muscle. Copyright © 2007 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/77693
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorVu, Ven_HK
dc.contributor.authorKim, Wen_HK
dc.contributor.authorFang, Xen_HK
dc.contributor.authorLiu, YTen_HK
dc.contributor.authorXu, Aen_HK
dc.contributor.authorSweeney, Gen_HK
dc.date.accessioned2010-09-06T07:34:41Z-
dc.date.available2010-09-06T07:34:41Z-
dc.date.issued2007en_HK
dc.identifier.citationEndocrinology, 2007, v. 148 n. 9, p. 4411-4419en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77693-
dc.description.abstractWe developed a coculture system comprising primary rat adipocytes and L6 rat skeletal muscle cells to allow investigation of the effects of physiologically relevant mixtures of adipokines. We observed that coculture, or adipocyte-conditioned media, increased glucose uptake in muscle cells. An adipokine that could potentially mediate this effect is adiponectin, and wedemonstrated that small interfering RNA-mediated knock-down of adiponectin receptor-2 in muscle cells reduced the uptake of glucose upon coculture with primary rat adipocytes. Analysis of coculture media by ELISA indicated total adiponectin concentration of up to 1 μg/ml, and Western blotting and gel filtration analysis demonstrated that the adipokine profile was hexamer greater than high molecular weight much greater than trimer. We used the streptozotocin-induced rat model of diabetes and found that high-molecularweight adiponectin levels decreased in comparison with control animals and this correlated with the fact that diabetic rat-derived primary adipocytes in coculture did not stimulate glucose uptake to the same extent as control adipocytes. Coculture induced phosphorylation of AMP-activated protein kinase (T172) and interestingly also insulin receptor substrate-1 (Y612) and Akt (T308 & S473), which could be attenuated after adiponectin receptor-2-small interfering RNA treatment. In summary, we believe that this coculture system represents an excellent model to study the effects of primary adipocyte-derived adipokine mixtures on skeletal muscle metabolism, and here we have established that in the context of physiologically relevant mixtures of adipokines, adiponectin may be an important determinant of positive cross talk between adipocytes and skeletal muscle. Copyright © 2007 by The Endocrine Society.en_HK
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.rightsEndocrinology. Copyright © The Endocrine Society.en_HK
dc.subject.meshAdipocytes - cytology - physiologyen_HK
dc.subject.meshAdiponectin - physiologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBiological Transporten_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCoculture Techniquesen_HK
dc.subject.meshDiabetes Mellitus, Experimental - pathology - physiopathologyen_HK
dc.subject.meshEpididymisen_HK
dc.subject.meshGlucose - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMuscle, Skeletal - metabolismen_HK
dc.subject.meshRNA, Small Interfering - geneticsen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Wistaren_HK
dc.subject.meshReceptors, Adiponectinen_HK
dc.subject.meshReceptors, Cell Surface - geneticsen_HK
dc.titleCoculture with primary visceral rat adipocytes from control but not streptozotocin-induced diabetic animals increases glucose uptake in rat skeletal muscle cells: Role of adiponectinen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=148&spage=4411&epage=9&date=2007&atitle=Coculture+with+primary+visceral+rat+adipocytes+from+control+but+not+streptozotocin-induced+diabetic+animals+increases+glucose+uptake+in+rat+skeletal+muscle+cells:+role+of+adiponectinen_HK
dc.identifier.emailXu, A:amxu@hkucc.hku.hken_HK
dc.identifier.authorityXu, A=rp00485en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/en.2007-0020en_HK
dc.identifier.pmid17569760-
dc.identifier.scopuseid_2-s2.0-34548087570en_HK
dc.identifier.hkuros140647en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548087570&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume148en_HK
dc.identifier.issue9en_HK
dc.identifier.spage4411en_HK
dc.identifier.epage4419en_HK
dc.identifier.isiWOS:000248789400035-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridVu, V=36448715400en_HK
dc.identifier.scopusauthoridKim, W=19638966100en_HK
dc.identifier.scopusauthoridFang, X=10642149900en_HK
dc.identifier.scopusauthoridLiu, YT=23501743300en_HK
dc.identifier.scopusauthoridXu, A=7202655409en_HK
dc.identifier.scopusauthoridSweeney, G=7102852659en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats