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Article: Ion channels in mesenchymal stem cells from rat bone marrow

TitleIon channels in mesenchymal stem cells from rat bone marrow
Authors
KeywordsCa 2+-activated K + current voltage-gated K + current
Rat mesenchymal stem cells
Tetrodotoxin-sensitive Na + current
Transient outward K + current
Issue Date2006
PublisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.com
Citation
Stem Cells, 2006, v. 24 n. 6, p. 1519-1528 How to Cite?
AbstractMesenchymal stem cells (MSCs) from bone marrow are believed to be an ideal cell source for cardiomyoplasty; however, cellular electrophysiology is not understood. The present study was designed to investigate ion channels in undifferentiated rat MSCs. It was found that three types of outward currents were present in rat MSCs, including a small portion of Ca 2+-activated K + channel (I KCa) sensitive to inhibition by iberiotoxin and/or clotromazole, a delayed rectifier K + current (IK DR), and a transient outward K + current (I to). In addition, tetrodotoxin (TTX)-sensitive sodium current (I Na.TTX) and nifedipine-sensitive L-type Ca 2+ current (I Ca.L) were found in a small population of rat MSCs. Moreover, reverse transcription- polymerase chain reaction revealed the molecular evidence of mRNA for the functional ionic currents, including Slo and KCNN4 for I KCa; Kv1.4 for I to; Kv1.2 and Kv2.1 for IK DR; SCN2a1 for I Na.TTX; and CCHL2a for I Ca.L. These results demonstrate for the first time that multiple functional ion channel currents (i.e., I KCa, I to, IK DR, I Na.TTX, and I Ca.L) are present in rat MSCs from bone marrow; however, physiological roles of these ion channels remain to be studied. ©AlphaMed Press.
Persistent Identifierhttp://hdl.handle.net/10722/77632
ISSN
2015 Impact Factor: 5.902
2015 SCImago Journal Rankings: 3.438
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, GRen_HK
dc.contributor.authorDeng, XLen_HK
dc.contributor.authorSun, Hen_HK
dc.contributor.authorChung, SSMen_HK
dc.contributor.authorTse, HFen_HK
dc.contributor.authorLau, CPen_HK
dc.date.accessioned2010-09-06T07:34:01Z-
dc.date.available2010-09-06T07:34:01Z-
dc.date.issued2006en_HK
dc.identifier.citationStem Cells, 2006, v. 24 n. 6, p. 1519-1528en_HK
dc.identifier.issn1066-5099en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77632-
dc.description.abstractMesenchymal stem cells (MSCs) from bone marrow are believed to be an ideal cell source for cardiomyoplasty; however, cellular electrophysiology is not understood. The present study was designed to investigate ion channels in undifferentiated rat MSCs. It was found that three types of outward currents were present in rat MSCs, including a small portion of Ca 2+-activated K + channel (I KCa) sensitive to inhibition by iberiotoxin and/or clotromazole, a delayed rectifier K + current (IK DR), and a transient outward K + current (I to). In addition, tetrodotoxin (TTX)-sensitive sodium current (I Na.TTX) and nifedipine-sensitive L-type Ca 2+ current (I Ca.L) were found in a small population of rat MSCs. Moreover, reverse transcription- polymerase chain reaction revealed the molecular evidence of mRNA for the functional ionic currents, including Slo and KCNN4 for I KCa; Kv1.4 for I to; Kv1.2 and Kv2.1 for IK DR; SCN2a1 for I Na.TTX; and CCHL2a for I Ca.L. These results demonstrate for the first time that multiple functional ion channel currents (i.e., I KCa, I to, IK DR, I Na.TTX, and I Ca.L) are present in rat MSCs from bone marrow; however, physiological roles of these ion channels remain to be studied. ©AlphaMed Press.en_HK
dc.languageengen_HK
dc.publisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.comen_HK
dc.relation.ispartofStem Cellsen_HK
dc.subjectCa 2+-activated K + current voltage-gated K + currenten_HK
dc.subjectRat mesenchymal stem cellsen_HK
dc.subjectTetrodotoxin-sensitive Na + currenten_HK
dc.subjectTransient outward K + currenten_HK
dc.subject.meshAdipogenesisen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBone Marrow Cells - cytology - metabolismen_HK
dc.subject.meshCardiomyoplastyen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshIon Channels - classification - genetics - metabolismen_HK
dc.subject.meshMembrane Potentialsen_HK
dc.subject.meshMesenchymal Stem Cells - cytology - metabolismen_HK
dc.subject.meshMyocardium - cytology - metabolismen_HK
dc.subject.meshOsteogenesisen_HK
dc.subject.meshRNA, Messenger - genetics - metabolismen_HK
dc.subject.meshRatsen_HK
dc.titleIon channels in mesenchymal stem cells from rat bone marrowen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1066-5099&volume=24&issue=6&spage=1519&epage=1528&date=2006&atitle=Ion+channels+in+mesenchymal+stem+cells+from+rat+bone+marrow.en_HK
dc.identifier.emailLi, GR: grli@hkucc.hku.hken_HK
dc.identifier.emailChung, SSM: smchung@hkucc.hku.hken_HK
dc.identifier.emailTse, HF: hftse@hkucc.hku.hken_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.identifier.authorityChung, SSM=rp00376en_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1634/stemcells.2005-0307en_HK
dc.identifier.pmid16484345-
dc.identifier.scopuseid_2-s2.0-33746820497en_HK
dc.identifier.hkuros117045en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33746820497&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1519en_HK
dc.identifier.epage1528en_HK
dc.identifier.eissn1549-4918-
dc.identifier.isiWOS:000240639300016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, GR=7408462932en_HK
dc.identifier.scopusauthoridDeng, XL=14057894600en_HK
dc.identifier.scopusauthoridSun, H=7404828399en_HK
dc.identifier.scopusauthoridChung, SSM=14120761600en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.scopusauthoridLau, CP=7401968501en_HK

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