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Article: Platelet transmembrane signalling responses to collagen in familial hypercholesterolaemia

TitlePlatelet transmembrane signalling responses to collagen in familial hypercholesterolaemia
Authors
Issue Date1994
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/ECI
Citation
European Journal Of Clinical Investigation, 1994, v. 24 n. 11, p. 737-743 How to Cite?
AbstractWashed platelets from patients with familial hypercholesterolaemia (FH) were found to be more reactive towards collagen than those from control subjects. The dose required to achieve half maximum aggregation was found to be 0.6 ml-1 for FH patients whilst that for control subjects was 1.25 μg ml-1. In both types of platelet, intracellular Ca2+ levels, as monitored by the Ca2+-dependent photoprotein, aequorin, rose on stimulation with collagen and then fell to basal levels, probably due to resequestration by the reticular system. This effect was not due to exhaustion of the supply of aequorin since sustained Ca2+ influx induced by the ionophore, A23187, gave a stable signal that did not return to baseline. Similarly, inositol 1,4,5, trisphosphate levels increased in the cytosol after stimulation and then fell to unstimulated values. When stimulated with collagen, platelets from FH patients showed a greater extent of cytoplasmic calcium mobilization (P < 0.05) when compared to controls, coupled with a greater extent of inositol phospholipid hydrolysis (P < 0.05). At doses of collagen sufficient to give either 100% or 50% aggregation, platelets from patients or control subjects showed the same amplitude of ATP release at either dose suggesting that the trigger for vesicle release is more sensitive in FH.
Persistent Identifierhttp://hdl.handle.net/10722/77564
ISSN
2015 Impact Factor: 2.687
2015 SCImago Journal Rankings: 1.255
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCooper, MBen_HK
dc.contributor.authorTan, KCBen_HK
dc.contributor.authorBetteridge, DJen_HK
dc.date.accessioned2010-09-06T07:33:16Z-
dc.date.available2010-09-06T07:33:16Z-
dc.date.issued1994en_HK
dc.identifier.citationEuropean Journal Of Clinical Investigation, 1994, v. 24 n. 11, p. 737-743en_HK
dc.identifier.issn0014-2972en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77564-
dc.description.abstractWashed platelets from patients with familial hypercholesterolaemia (FH) were found to be more reactive towards collagen than those from control subjects. The dose required to achieve half maximum aggregation was found to be 0.6 ml-1 for FH patients whilst that for control subjects was 1.25 μg ml-1. In both types of platelet, intracellular Ca2+ levels, as monitored by the Ca2+-dependent photoprotein, aequorin, rose on stimulation with collagen and then fell to basal levels, probably due to resequestration by the reticular system. This effect was not due to exhaustion of the supply of aequorin since sustained Ca2+ influx induced by the ionophore, A23187, gave a stable signal that did not return to baseline. Similarly, inositol 1,4,5, trisphosphate levels increased in the cytosol after stimulation and then fell to unstimulated values. When stimulated with collagen, platelets from FH patients showed a greater extent of cytoplasmic calcium mobilization (P < 0.05) when compared to controls, coupled with a greater extent of inositol phospholipid hydrolysis (P < 0.05). At doses of collagen sufficient to give either 100% or 50% aggregation, platelets from patients or control subjects showed the same amplitude of ATP release at either dose suggesting that the trigger for vesicle release is more sensitive in FH.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/ECIen_HK
dc.relation.ispartofEuropean Journal of Clinical Investigationen_HK
dc.rightsEuropean Journal of Clinical Investigation. Copyright © Blackwell Publishing Ltd.en_HK
dc.subject.meshAdulten_HK
dc.subject.meshAequorin - metabolismen_HK
dc.subject.meshBlood Platelets - drug effects - metabolism - physiologyen_HK
dc.subject.meshCalcium - metabolismen_HK
dc.subject.meshCholesterol - blooden_HK
dc.subject.meshCollagen - pharmacologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHyperlipoproteinemia Type II - blood - physiopathologyen_HK
dc.subject.meshInositol 1,4,5-Trisphosphate - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshPlatelet Aggregation - drug effects - physiologyen_HK
dc.titlePlatelet transmembrane signalling responses to collagen in familial hypercholesterolaemiaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-2972&volume=24&spage=737&epage=743&date=1994&atitle=Platelet+transmembrane+signalling+responses+to+collagen+in+familial+hypercholesterolaemiaen_HK
dc.identifier.emailTan, KCB:kcbtan@hku.hken_HK
dc.identifier.authorityTan, KCB=rp00402en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid7890011-
dc.identifier.scopuseid_2-s2.0-0028143397en_HK
dc.identifier.hkuros4789en_HK
dc.identifier.volume24en_HK
dc.identifier.issue11en_HK
dc.identifier.spage737en_HK
dc.identifier.epage743en_HK
dc.identifier.isiWOS:A1994PU33500004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridCooper, MB=7404410514en_HK
dc.identifier.scopusauthoridTan, KCB=8082703100en_HK
dc.identifier.scopusauthoridBetteridge, DJ=34973752700en_HK

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