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Article: Detection of T-cell receptor delta gene rearrangement in T-cell malignancies by clonal specific polymerase chain reaction and its application to detect minimal residual disease

TitleDetection of T-cell receptor delta gene rearrangement in T-cell malignancies by clonal specific polymerase chain reaction and its application to detect minimal residual disease
Authors
KeywordsAcute lymphoblastic leukemia
Non-Hodgkin's lymphoma
Polymerase chain reaction
T-cell receptor delta
Issue Date1996
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35105
Citation
American Journal Of Hematology, 1996, v. 52 n. 3, p. 171-177 How to Cite?
AbstractA clonal-specific polymerase chain reaction technique to detect T-cell receptor delta gene rearrangement in acute lymphoblastic leukaemia (ALL) and non-Hodgkin's lymphoma (NHL) was evaluated. It was applied to detect minimal residual disease. A sensitive and specific technique to detect minimal residual disease for T-cell malignancies was explored. Southern analysis and polymerase chain reaction (PCR) were used to detect the rearranged V-D-J segment of T-cell receptor delta (TCRδ) gene from malignant cell specimens of patients with leukemia and lymphoma of T-cell lineage. The PCR product was sequenced and from the DNA sequences of the V-D-J region, a 3' anti-sense primer was designed and synthesized for clonal specific PCR (CS-PCR). T-cell receptor delta (TCRδ) gene rearrangement was studied in 40 cases of acute leukaemia and lymphoma of T-cell lineage at diagnosis. Using Southern analysis, the positive rates were 28 and 32% for the 18 T-lymphoma and 22 T-ALL, respectively. A one stage Polymerase Chain Reaction (PCR) technique was used to detect the rearrangement in Southern positive cases and the PCR positive rates were 80 and 86%, respectively. The PCR technique had a sensitivity of 0.1%. Serial follow-up marrow specimens were available from 4 T-ALL patients following chemotherapy for monitoring of minimal residual disease. Their PCR products were DNA sequenced. A 3' primer was designed for each case for a clonal specific (CS) PCR. The technique had a sensitivity of 0.003%, it was applied to detect minimal residual disease in serial follow-up marrow samples. The first patient had persistent negative CS-PCR results and enjoyed continuous remission for more than 3 years. The second patient with negative one stage PCR but positive CS-PCR results had eventual relapse of leukaemia. The other two patients never achieved a morphological remission. These preliminary results appeared to support the usefulness of these PCR techniques in detecting minimal residual disease and predicting relapses for ALL. However, further clinical correlation in larger populations of patients is necessary.
Persistent Identifierhttp://hdl.handle.net/10722/77422
ISSN
2015 Impact Factor: 5.0
2015 SCImago Journal Rankings: 1.761
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, DWen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorChan, Ven_HK
dc.date.accessioned2010-09-06T07:31:43Z-
dc.date.available2010-09-06T07:31:43Z-
dc.date.issued1996en_HK
dc.identifier.citationAmerican Journal Of Hematology, 1996, v. 52 n. 3, p. 171-177en_HK
dc.identifier.issn0361-8609en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77422-
dc.description.abstractA clonal-specific polymerase chain reaction technique to detect T-cell receptor delta gene rearrangement in acute lymphoblastic leukaemia (ALL) and non-Hodgkin's lymphoma (NHL) was evaluated. It was applied to detect minimal residual disease. A sensitive and specific technique to detect minimal residual disease for T-cell malignancies was explored. Southern analysis and polymerase chain reaction (PCR) were used to detect the rearranged V-D-J segment of T-cell receptor delta (TCRδ) gene from malignant cell specimens of patients with leukemia and lymphoma of T-cell lineage. The PCR product was sequenced and from the DNA sequences of the V-D-J region, a 3' anti-sense primer was designed and synthesized for clonal specific PCR (CS-PCR). T-cell receptor delta (TCRδ) gene rearrangement was studied in 40 cases of acute leukaemia and lymphoma of T-cell lineage at diagnosis. Using Southern analysis, the positive rates were 28 and 32% for the 18 T-lymphoma and 22 T-ALL, respectively. A one stage Polymerase Chain Reaction (PCR) technique was used to detect the rearrangement in Southern positive cases and the PCR positive rates were 80 and 86%, respectively. The PCR technique had a sensitivity of 0.1%. Serial follow-up marrow specimens were available from 4 T-ALL patients following chemotherapy for monitoring of minimal residual disease. Their PCR products were DNA sequenced. A 3' primer was designed for each case for a clonal specific (CS) PCR. The technique had a sensitivity of 0.003%, it was applied to detect minimal residual disease in serial follow-up marrow samples. The first patient had persistent negative CS-PCR results and enjoyed continuous remission for more than 3 years. The second patient with negative one stage PCR but positive CS-PCR results had eventual relapse of leukaemia. The other two patients never achieved a morphological remission. These preliminary results appeared to support the usefulness of these PCR techniques in detecting minimal residual disease and predicting relapses for ALL. However, further clinical correlation in larger populations of patients is necessary.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35105en_HK
dc.relation.ispartofAmerican Journal of Hematologyen_HK
dc.rightsAmerican Journal of Hematology. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectAcute lymphoblastic leukemiaen_HK
dc.subjectNon-Hodgkin's lymphomaen_HK
dc.subjectPolymerase chain reactionen_HK
dc.subjectT-cell receptor deltaen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBlotting, Southernen_HK
dc.subject.meshBone Marrow - pathology - physiopathologyen_HK
dc.subject.meshClone Cellsen_HK
dc.subject.meshFollow-Up Studiesen_HK
dc.subject.meshGene Rearrangementen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLymphoma, Non-Hodgkin - geneticsen_HK
dc.subject.meshMolecular Probes - geneticsen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNeoplasm, Residual - diagnosisen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshPrecursor Cell Lymphoblastic Leukemia-Lymphoma - geneticsen_HK
dc.subject.meshReceptors, Antigen, T-Cell, gamma-delta - geneticsen_HK
dc.subject.meshRemission Inductionen_HK
dc.titleDetection of T-cell receptor delta gene rearrangement in T-cell malignancies by clonal specific polymerase chain reaction and its application to detect minimal residual diseaseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0361-8609&volume=52&spage=171&epage=177&date=1996&atitle=Detection+of+T-cell+receptor+delta+gene+rearrangement+in+T-cell+malignancies+by+clonal+specific+polymerase+chain+reaction+and+its+application+to+detect+minimal+residual+disease.en_HK
dc.identifier.emailChan, DW:dwchan@hkucc.hku.hken_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.emailChan, V:vnychana@hkucc.hku.hken_HK
dc.identifier.authorityChan, DW=rp00543en_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.authorityChan, V=rp00320en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1096-8652(199607)52:3<171::AID-AJH6>3.0.CO;2-Qen_HK
dc.identifier.pmid8756082-
dc.identifier.scopuseid_2-s2.0-0029680225en_HK
dc.identifier.hkuros22797en_HK
dc.identifier.hkuros12278-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029680225&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue3en_HK
dc.identifier.spage171en_HK
dc.identifier.epage177en_HK
dc.identifier.isiWOS:A1996UY81400008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, DW=26533900600en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridChan, V=7202654865en_HK

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